Analysis Tools
mortality/aging
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• highly sensitive to radiation treatment
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• about 75% of mice die by 4 weeks of age
• only 1 of 56 mice survived past 12 weeks of age
• bone marrow transplant from wild-type littermates at 2 weeks of age rescues much of the lethality
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• about 75% of mice die by 4 weeks of age
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hematopoietic system
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• increase in nucleotide loss during coding joint formation similar to mice homozygous for Prkdcscid
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• apparent arrest at the pro-B cell stage
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• generalized decrease in the number of leukocytes
• average number of nucleated cells is reduced at 2 weeks of age
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• in the peripheral blood
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• in the peripheral blood
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• in the peripheral blood
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• in the bone marrow
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• drastically reduced lymphocyte numbers at 2 weeks of age
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• few mature B cells are found in the spleen
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• unlike in null mice, double positive and single positive T cells are present although in reduced numbers
• decrease in the number of cells expressing TCRB
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• severe reduction of double-negative thymocytes at different developmental stages
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• in the bone marrow
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• decrease in the number of (Lin-Sca-1+c-Kit+ (LSK) cells in the liver at E14.5
• severe reduction (100 fold) in the number of bone marrow LSK cells at P1
• near ablation of bone marrow LSK cells at 2 weeks of age
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pancytopenia
(
J:171351
)
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• in the peripheral blood beginning at 2 weeks of age
• bone marrow transplant from wild-type littermates at 2 weeks rescues pancytopenia
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• cells isolated at E12.5 proliferate normally for 24h then slow down and collapse due to massive cell death by 72h in culture
• fetal liver LSK cells are unable to repopulate in vivo
• fetal liver LSK cells show an increase in spontaneous DNA lesions and apoptosis
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cellular
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• MEFs display increased sensitivity to replication stress inducers, including UV irradiation, camptothecin, and mitomycin C
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• most striking response is to mitomycin C
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• highly sensitive to radiation treatment
• MEFs are hypersensitive to ionizing radiation induced cell death
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• expression analysis in MEFs following DNA damage indicates abnormalities in the homologous recombination/Fanconi anemia pathway
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growth/size/body
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• become apparently smaller within 2?3 weeks of birth
• bone marrow transplant from wild-type littermates at 2 weeks of age improves growth
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immune system
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• increase in nucleotide loss during coding joint formation similar to mice homozygous for Prkdcscid
|
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• apparent arrest at the pro-B cell stage
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• in the peripheral blood
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• in the bone marrow
|
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• drastically reduced lymphocyte numbers at 2 weeks of age
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• few mature B cells are found in the spleen
|
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• unlike in null mice, double positive and single positive T cells are present although in reduced numbers
• decrease in the number of cells expressing TCRB
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• severe reduction of double-negative thymocytes at different developmental stages
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• in the bone marrow
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• drastically attenuated at 2 weeks of age
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integument
digestive/alimentary system
homeostasis/metabolism
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• expression analysis in MEFs following DNA damage indicates abnormalities in the homologous recombination/Fanconi anemia pathway
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• highly sensitive to radiation treatment
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pigmentation
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• hyperpigmentation of the footpad and tail apparent by 1 week of age
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