Phenotypes associated with this allele

• Allele Symbol: Col5a3^tm1Dgr
• Allele Name: targeted mutation 1, Daniel S Greenspan
• Allele ID: MGI:5000519
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Col5a3^tm1Dgr/Col5a3^tm1Dgr	involves: 129S1/Sv * 129X1/SvJ * ICR	MGI:5000520

Genotype
MGI:5000520

hm1

• Allelic Composition: Col5a3^tm1Dgr/Col5a3^tm1Dgr
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * ICR

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Reduced thickness of the adipocyte-rich hypodermal layer of Col5a3^tm1Dgr/Col5a3^tm1Dgr females

endocrine/exocrine glands

decreased pancreatic beta cell mass ( J:171817 )

• relative area of beta cells and islet density are significantly reduced in newborns

• relative area of beta cells is decreased at 3 months and 1 year of age

decreased pancreatic beta cell number ( J:171817 )

• at 8 to 12 weeks of age the beta cell area is reduced due to reduced islet numbers

decreased pancreas weight ( J:171817 )

abnormal pancreas physiology ( J:171817 )

• at 1 year of age islets are friable unlike islets from wild-type mice

increased pancreatic islet cell apoptosis ( J:171817 )

• at 1 year of age about 3 fold more apoptotic cells are seen in pancreatic islets

increased pancreatic beta cell apoptosis ( J:171817 )

• marked increase in the numbers of streptozotocin induced apoptotic beta cells

decreased insulin secretion ( J:171817 )

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:171817 )

• total body weights are lower in females on a high fat diet, but not on a normal chow diet, compared to diet matched wild-type females

abnormal glucose homeostasis ( J:171817 )

decreased insulin secretion ( J:171817 )

hyperglycemia ( J:171817 )

• in males and females at 3 months of age and in newborns

• at 1 year of age fasting glucose levels increase to near diabetic levels

decreased circulating insulin level ( J:171817 )

• in males and females at 3 months and 1 year of age

impaired glucose tolerance ( J:171817 )

• in males and females at 3 months of age

• glucose intolerance appears worse at 1 year of age compared to 3 months

insulin resistance ( J:171817 )

• in males and females at 3 months of age

• insulin resistance appears worse at 1 year of age compared to 3 months

increased physiological sensitivity to xenobiotic ( J:171817 )

• marked increase in the numbers of streptozotocin induced apoptotic beta cells

• marked loss of body weight in streptozotocin treated mutants compared to similarly treated controls

adipose tissue

decreased subcutaneous adipose tissue amount ( J:171817 )

• in females at 10 days of age

decreased adipocyte glucose uptake ( J:171817 )

• decrease in glucose uptake in adipocytes even in the presence of relatively low insulin levels

• defect in glucose uptake increases with age

muscle

decreased skeletal muscle cell glucose uptake ( J:171817 )

• decrease in glucose uptake in response to 100 nM insulin in soleus muscle cells from 10 to 12 week old mice

• defect in glucose uptake increases with age

growth/size/body

decreased body weight ( J:171817 )

• total body weights are lower in females on a high fat diet, but not on a normal chow diet, compared to diet matched wild-type females

weight loss ( J:171817 )

• marked loss of body weight in streptozotocin treated mutants compared to similarly treated controls

decreased susceptibility to diet-induced obesity ( J:171817 )

• total body weights are lower in females on a high fat diet, but not on a normal chow diet, compared to diet matched wild-type females

behavior/neurological

behavior/neurological phenotype ( J:171817 )

N • no differences in food intake are detected

integument

decreased subcutaneous adipose tissue amount ( J:171817 )

• in females at 10 days of age

cellular

decreased adipocyte glucose uptake ( J:171817 )

• decrease in glucose uptake in adipocytes even in the presence of relatively low insulin levels

• defect in glucose uptake increases with age

increased pancreatic islet cell apoptosis ( J:171817 )

• at 1 year of age about 3 fold more apoptotic cells are seen in pancreatic islets

increased pancreatic beta cell apoptosis ( J:171817 )

• marked increase in the numbers of streptozotocin induced apoptotic beta cells

decreased skeletal muscle cell glucose uptake ( J:171817 )

• decrease in glucose uptake in response to 100 nM insulin in soleus muscle cells from 10 to 12 week old mice

• defect in glucose uptake increases with age