Phenotypes associated with this allele

• Allele Symbol: Rasip1^tm1.1Oncl
• Allele Name: targeted mutation 1.1, Ondine Cleaver
• Allele ID: MGI:5056095
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rasip1^tm1.1Oncl/Rasip1^tm1.1Oncl	involves: C57BL/6 * CBA	MGI:5056104
cx2	Kdr^tm1Jrt/Kdr^+ Rasip1^tm1.1Oncl/Rasip1^tm1.1Oncl	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA	MGI:5056105

Genotype
MGI:5056104

hm1

• Allelic Composition: Rasip1^tm1.1Oncl/Rasip1^tm1.1Oncl
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:174152 )

• mice die by E10.5

cardiovascular system

abnormal cardiovascular development ( J:174152 )

• the inflow tract lacks continuous lumen unlike in wild-type mice

abnormal vascular development ( J:174152 )

• at E8.0 to E8.5, preaortal angioblast align in narrow rows with visible occlusions compared to in wild-type mice

• arteriovenous differentiation fails unlike in wild-type mice

• blood vessels such as the dorsal aortae, endocardium, yolk sac, vitelline artery, inflow tract, and allantois lack continuous lumens unlike in wild-type mice

• mice exhibit failure of tubulogenesis at the onset of vessel formation unlike wild-type mice

• however, mice exhibit normal angioblast specification, proliferation, and patterning

abnormal dorsal aorta morphology ( J:174152 )

• lacks continuous lumen

abnormal vascular endothelial cell development ( J:174152 )

• tight junction are clustered at the center of endothelial cords and adherens junctions are ectopically distributed unlike in wild-type mice

• endothelial cells fail to exhibit adhesion to extracellular matrix and fail to establish apicobasal polarity unlike in wild-type mice

abnormal vascular endothelial cell differentiation ( J:174152 )

• at E8.5, aortic endothelial cells remain cuboidal unlike in wild-type mice

abnormal vitelline vasculature morphology ( J:174152 )

• yolk sacs lack the hierarchical vascular network observed in wild-type mice

• yolk sacs and the vitelline artery lack continuous lumen unlike in wild-type mice

abnormal endocardium morphology ( J:174152 )

• lacks continuous lumen

embryo

abnormal vitelline vasculature morphology ( J:174152 )

• yolk sacs lack the hierarchical vascular network observed in wild-type mice

• yolk sacs and the vitelline artery lack continuous lumen unlike in wild-type mice

embryonic growth retardation ( J:174152 )

• at E9.5

abnormal allantois morphology ( J:174152 )

• lacks continuous lumen

homeostasis/metabolism

edema ( J:174152 )

• at E9.5

growth/size/body

embryonic growth retardation ( J:174152 )

• at E9.5

cellular

abnormal vascular endothelial cell differentiation ( J:174152 )

• at E8.5, aortic endothelial cells remain cuboidal unlike in wild-type mice

Genotype
MGI:5056105

cx2

• Allelic Composition: Kdr^tm1Jrt/Kdr⁺; Rasip1^tm1.1Oncl/Rasip1^tm1.1Oncl
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA

cardiovascular system

abnormal vasculogenesis ( J:174152 )

• at E9.5, mice fail to exhibit remodeling of the initial plexus in embryonic tissue unlike wild-type mice

embryo

abnormal vitelline vascular remodeling ( J:174152 )

• at E9.5, mice fail to exhibit remodeling of the initial plexus in the yolk sac unlike wild-type mice