All Phenotypes Slc32a1<tm2(cre)Lowl> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Slc32a1^tm2(cre)Lowl/Slc32a1^tm2(cre)Lowl	either: B6J.129S6(FVB)-Slc32a1^tm2(cre)Lowl or (involves: 129S6/SvEvTac * C57BL/6J * FVB/N)	MGI:6730293
ht2	Slc32a1^tm2(cre)Lowl/Slc32a1⁺	involves: 129S6/SvEvTac	MGI:5141285
cn3	Mc4r^tm1Lowl/Mc4r^tm1Lowl Slc32a1^tm2(cre)Lowl/Slc32a1⁺	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * FVB/N	MGI:5609682
cn4	Lepr^tm1.1Chua/Lepr^tm1.1Chua Slc32a1^tm2(cre)Lowl/Slc32a1⁺	involves: 129S6/SvEvTac	MGI:5142146
cn5	Prkar2b^tm3Gsm/Prkar2b^tm2Gsm Slc32a1^tm2(cre)Lowl/Slc32a1⁺	involves: 129S6/SvEvTac * C57BL/6	MGI:5515339
cn6	Abtb3^{tm1c(EUCOMM)Wtsi}/Abtb3^{tm1c(EUCOMM)Wtsi} Slc32a1^tm2(cre)Lowl/Slc32a1⁺	involves: 129S6/SvEvTac * C57BL/6 * FVB/N	MGI:7524105
cn7	Lepr^tm1.1Chua/Lepr^tm1.1Chua Slc32a1^tm2(cre)Lowl/Slc32a1⁺ Tg(Pomc1-hrGFP)1Lowl/0	involves: 129S6/SvEvTac * FVB/N	MGI:5142225

Allelic Composition	Slc32a1^tm2(cre)Lowl/Slc32a1^tm2(cre)Lowl
Genetic Background	either: B6J.129S6(FVB)-Slc32a1^tm2(cre)Lowl or (involves: 129S6/SvEvTac * C57BL/6J * FVB/N)

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc32a1^tm2(cre)Lowl mutation (2 available); any Slc32a1 mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

increased kindling response ( J:308516 )

• mice develop spontaneous recurring limbic seizures about 1 week after electrical kindling, although in some cases seizures began before mice were fully kindled

tonic-clonic seizures ( J:308516 )

• electrical kindling induces spontaneous recurring seizures after a short latency in about 80% of mice; seizures occur 1-2 times per day and most are bilateral, tonic-clonic seizures

• however, no to little neuronal death is seen

abnormal inhibitory postsynaptic currents ( J:308516 )

• spontaneous IPSCs show a decrease in amplitude in CA1 neurons and dentate granule cells, with an average decrease of 23% in CA1 neurons

• however, no differences in spontaneous IPSC rise time, decay rate, or frequency are seen

behavior/neurological

increased kindling response ( J:308516 )

• mice develop spontaneous recurring limbic seizures about 1 week after electrical kindling, although in some cases seizures began before mice were fully kindled

tonic-clonic seizures ( J:308516 )

• electrical kindling induces spontaneous recurring seizures after a short latency in about 80% of mice; seizures occur 1-2 times per day and most are bilateral, tonic-clonic seizures

• however, no to little neuronal death is seen

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
temporal lobe epilepsy		DOID:3328	OMIM:PS600512	J:308516

Allelic Composition	Slc32a1^tm2(cre)Lowl/Slc32a1⁺
Genetic Background	involves: 129S6/SvEvTac

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc32a1^tm2(cre)Lowl mutation (2 available); any Slc32a1 mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

normal phenotype

no abnormal phenotype detected ( J:174691 )

• heterozygotes do not display a detectable abnormal phenotype

Allelic Composition	Mc4r^tm1Lowl/Mc4r^tm1Lowl Slc32a1^tm2(cre)Lowl/Slc32a1⁺
Genetic Background	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6 * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Mc4r^tm1Lowl mutation (2 available); any Mc4r mutation (48 available)
Slc32a1^tm2(cre)Lowl mutation (2 available); any Slc32a1 mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

obese ( J:216397 )

Allelic Composition	Lepr^tm1.1Chua/Lepr^tm1.1Chua Slc32a1^tm2(cre)Lowl/Slc32a1⁺
Genetic Background	involves: 129S6/SvEvTac

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lepr^tm1.1Chua mutation (1 available); any Lepr mutation (122 available)
Slc32a1^tm2(cre)Lowl mutation (2 available); any Slc32a1 mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

growth/size/body

increased body weight ( J:174691 )

• males and females exhibit a massive increase in body weight from 4 weeks of age through 7 weeks compared to controls

behavior/neurological

polyphagia ( J:174691 )

• food intake is greatly increased compared to controls

nervous system

abnormal nervous system electrophysiology ( J:174691 )

• Pomc1 neurons tend to be hyperpolarized relative to control neurons

abnormal inhibitory postsynaptic currents ( J:174691 )

• a large increase in amplitude and frequency of spontaneous IPSCs is observed in Pomc1 neurons in slice preparations from mice with conditional deletion of Lepr, indicating that deletion from presynaptic GABAergic neurons increases the inhibitory tone in Pomc1 neurons

homeostasis/metabolism

abnormal circulating glucose level ( J:174691 )

• animals have significantly elevated fed and fasting blood glucose levels

increased circulating insulin level ( J:174691 )

• animals have significantly elevated fed and fasted serum insulin levels

adipose tissue

increased total body fat amount ( J:174691 )

• animals display a massive increase in fat mass relative to controls at 10 weeks of age

Allelic Composition	Prkar2b^tm3Gsm/Prkar2b^tm2Gsm Slc32a1^tm2(cre)Lowl/Slc32a1⁺
Genetic Background	involves: 129S6/SvEvTac * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prkar2b^tm2Gsm mutation (1 available); any Prkar2b mutation (27 available)
Prkar2b^tm3Gsm mutation (0 available); any Prkar2b mutation (27 available)
Slc32a1^tm2(cre)Lowl mutation (2 available); any Slc32a1 mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:196157 )

N	• hyperactivity phenotype is reversed

growth/size/body

growth/size/body region phenotype ( J:196157 )

N	• lean body mass is similar to controls

adipose tissue

adipose tissue phenotype ( J:196157 )

N	• fat pad weights are similar to controls

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:196157 )

N	• serum leptin levels similar to controls

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

nervous system phenotype ( J:337062 )

N	• mice show no significant differences in the paired pulse ratio (PPR) recorded from parvalbumin-positive inhibitory interneurons (PV-INs), suggesting normal presynaptic release probabilities; moreover, the AMPA/NMDA ratio is normal in PV-INs

abnormal postsynaptic density morphology ( J:337062 )

• mice exhibit a smaller median Psd-95-GFP puncta area per dendritic region in PV-INs than control mice

• however, the density of Psd-95-GFP puncta (a proxy of glutamatergic synapse density) is normal in PV-INs

abnormal nervous system electrophysiology ( J:337062 )

• analysis of visually evoked gamma oscillations in the primary visual cortex (V1) showed that, when mice are presented with a gray screen, the gray/black screen ratio of both mean and peak 55-65 Hz activity is significantly higher than that in control mice, indicating increased power in the narrowband gamma frequency

decreased miniature excitatory postsynaptic current frequency ( J:337062 )

• mEPSC frequency is severely reduced in parvalbumin-positive inhibitory interneurons (PV-INs) in the primary visual cortex (V1), as recorded at -70 mV in the presence of TTX and gabazine

• however, mEPSC amplitude, half-maximal rise time and half-maximal decay time in PV-INs are equivalent to those in control mice

impaired synaptic physiology ( J:337062 )

• mice exhibit decreased glutamatergic synaptic activity in PV-INs

behavior/neurological

behavior/neurological phenotype ( J:337062 )

N	• both sexes show no significant changes in short-term spatial memory and contextual fear conditioning tests relative to control mice

enhanced behavioral response to xenobiotic ( J:337062 )

• following injection of glutamate N-methyl-D-aspartate (NMDA) receptor antagonist MK-801, mice show a significantly greater increase in locomotor activity than similarly treated controls, as determined by the total number of infrared beam breaks in an open field arena during the 30-60 min period post-injection

increased exploration in new environment ( J:337062 )

• both sexes exhibit significantly more rearing events during a 30-min period of open field exploration, indicating an increased exploratory drive

decreased anxiety-related response ( J:337062 , J:337062 )

• both sexes make significantly more beam breaks in the center region of an open field arena during the first 5 min of exploration, suggesting reduced anxiety (J:337062)

• in the elevated plus maze (EPM) test, mice exhibit a significantly higher ratio of entries to the extreme end of the open arm vs. entries into the closed arm and spend significantly less time freezing during the 5-min test (J:337062)

• in the hole-board test, male -- but not female -- mice make significantly more nose pokes than controls during a 3-min session (J:337062)

• in the marble burying task, male -- but not female -- mice bury significantly fewer marbles than controls (J:337062)

decreased freezing behavior ( J:337062 )

• both sexes spend significantly less time freezing on the elevated plus maze apparatus during the 5 min test

increased vertical activity ( J:337062 )

• both sexes exhibit significantly more rearing events during a 30-min period of open field exploration, indicating an increased exploratory drive

• however, locomotor activity is normal, as determined by the total number of infrared beam breaks in an open field arena during a 90-min period in the dark or a 30-min period in the light

Allelic Composition	Lepr^tm1.1Chua/Lepr^tm1.1Chua Slc32a1^tm2(cre)Lowl/Slc32a1⁺ Tg(Pomc1-hrGFP)1Lowl/0
Genetic Background	involves: 129S6/SvEvTac * FVB/N

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal inhibitory postsynaptic currents ( J:174691 )

• addition of leptin fails to reduce the frequency of sIPSCs (spontaneous inhibitory postsynaptic currents) in Pomc1 neurons, while in control slices, the frequency is reduced by about 40%

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