All Phenotypes Pax7<tm1(cre/ERT2)Gaka> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Pax7^{tm1(cre/ERT2)Gaka}
targeted mutation 1, Gabrielle Kardon
MGI:5141477

Summary

9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺ Gt(ROSA)26Sor^tm1(DTA)Mrc/Gt(ROSA)26Sor⁺	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6	MGI:5141594
cn2	2310065F04Rik^tm1.1Boet/2310065F04Rik^tm1.1Boet Ino80^tm1.1Jland/Ino80^tm1.1Jland Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6NCrl	MGI:7645256
cn3	Prmt7^tm1.1Rchd/Prmt7^tm1.1Rchd Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6NCrl	MGI:6314346
cn4	Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor^tm1(EYFP)Cos Pax7^{tm1(cre/ERT2)Gaka}/Pax7^{tm1(cre/ERT2)Gaka} Paxbp1^tm1.1Nju/Paxbp1^tm1.1Nju	involves: 129S6/SvEvTac * 129X1/SvJ * C57BL/6NCr	MGI:6827448
cn5	Gt(ROSA)26Sor^{tm4(EWSR1/ATF1)Mrc}/Gt(ROSA)26Sor⁺ Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺	involves: 129S6/SvEvTac * C57BL/6	MGI:5495319
cn6	Six1^tm2.1Mair/Six1^tm2.1Mair Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6Cr * C57BL/6NTac	MGI:5449865
cn7	Zfp422^em1Mode/Zfp422^em1Mode Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺	involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NCrl	MGI:6477293
cn8	Pax7^{tm1(cre/ERT2)Gaka}/0 Snhg15^tm1.1Nju/Snhg15^tm1.1Nju	involves: C57BL/6J * SJL/J	MGI:6478905
cx9	Gt(ROSA)26Sor^{tm1.1(DUX4*)Plj}/Gt(ROSA)26Sor⁺ Pax7^{tm1(cre/ERT2)Gaka}/?	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6NCrl	MGI:6120566

Allelic Composition	Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺ Gt(ROSA)26Sor^tm1(DTA)Mrc/Gt(ROSA)26Sor⁺
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^tm1(DTA)Mrc mutation (1 available); any Gt(ROSA)26Sor mutation (993 available)
Pax7^{tm1(cre/ERT2)Gaka} mutation (1 available); any Pax7 mutation (38 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

muscle

decreased satellite cell number ( J:174914 )

• 83-84% of satellite cells are ablated in the right tibialis anterior muscle after 5 doses of tamoxifen and 5days after muscle damage induced by BaCl2 injection

skeletal muscle fibrosis ( J:174914 )

• reduced fibroblast expansion by 52% 5 days after muscle injury in tamoxifen treated mice

impaired skeletal muscle regeneration ( J:174914 )

• 89% reduction in regenerating myofibers 5 days after muscle injury in tamoxifen treated mice

• muscle regeneration dramatically impaired 28 days after injury

• right tibialis anterior muscle entirely fibrotic at 28 days and uninjured left tibialis anterior is reduced by 38%

• similar result when damage induced with cardiotoxin, no recovery after 56 days

Allelic Composition	2310065F04Rik^tm1.1Boet/2310065F04Rik^tm1.1Boet Ino80^tm1.1Jland/Ino80^tm1.1Jland Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺
Genetic Background	involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * C57BL/6NCrl

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
2310065F04Rik^tm1.1Boet mutation (0 available); any 2310065F04Rik mutation (0 available)
Ino80^tm1.1Jland mutation (1 available); any Ino80 mutation (440 available)
Pax7^{tm1(cre/ERT2)Gaka} mutation (1 available); any Pax7 mutation (38 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

muscle

muscle phenotype ( J:306156 )

N	• adult mice treated with tamoxifen show complete abrogation of the increased number of Pax7+ muscle stem cells (satellite cells) seen in homozygous 2310065F04Rik^tm1.1Boet mice

Allelic Composition	Prmt7^tm1.1Rchd/Prmt7^tm1.1Rchd Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺
Genetic Background	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6NCrl

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax7^{tm1(cre/ERT2)Gaka} mutation (1 available); any Pax7 mutation (38 available)
Prmt7^tm1.1Rchd mutation (0 available); any Prmt7 mutation (41 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

muscle

abnormal muscle physiology ( J:271361 )

• satellite cells isolated from 8 month tamoxifen-treated mice exhibit premature senescence unlike control cells

impaired skeletal muscle regeneration ( J:271361 )

• tamoxifen-treated mice subjected to cardiotoxin-induced muscle injury exhibit smaller and poorly organized regenerating myofibers compared with control mice

cellular

early cellular replicative senescence ( J:271361 )

• satellite cells or cardiotoxin-injured tibialis anterior muscles isolated from 8 month tamoxifen-treated mice exhibit premature senescence unlike control cells

homeostasis/metabolism

abnormal response to injury ( J:271361 )

• cardiotoxin-injured tibialis anterior muscles isolated from 8 month tamoxifen-treated mice exhibit premature senescence unlike control cells

Allelic Composition	Gt(ROSA)26Sor^tm1(EYFP)Cos/Gt(ROSA)26Sor^tm1(EYFP)Cos Pax7^{tm1(cre/ERT2)Gaka}/Pax7^{tm1(cre/ERT2)Gaka} Paxbp1^tm1.1Nju/Paxbp1^tm1.1Nju
Genetic Background	involves: 129S6/SvEvTac * 129X1/SvJ * C57BL/6NCr

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^tm1(EYFP)Cos mutation (11 available); any Gt(ROSA)26Sor mutation (993 available)
Pax7^{tm1(cre/ERT2)Gaka} mutation (1 available); any Pax7 mutation (38 available)
Paxbp1^tm1.1Nju mutation (0 available); any Paxbp1 mutation (46 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

cellular

abnormal mitochondrial morphology ( J:304562 )

• fragmented mitochondria, largely restricted to perinuclear region, lacking interconnecting tubular network in activated muscle stem cells

abnormal respiratory electron transport chain ( J:304562 )

• defective cellular respiration and glycolysis

abnormal mitochondrial ATP synthesis coupled electron transport ( J:304562 )

• defective cellular respiration and glycolysis

decreased mitochondrial fission ( J:304562 )

• defective mitochondrial biogenesis in activated muscle stem cells

abnormal oxidative phosphorylation ( J:304562 )

• defective cellular respiration and glycolysis

muscle

impaired skeletal muscle regeneration ( J:304562 )

• virtual absence of regenerating myofibers 5 days after injection of cardiotoxin (CTX) into the tibialis anterior (TA) muscles and no muscle regeneration after 14 nor 30 days

• adipocyte infiltration 30 days after injection of cardiotoxin (CTX) into the tibialis anterior (TA) muscles

Allelic Composition	Gt(ROSA)26Sor^{tm4(EWSR1/ATF1)Mrc}/Gt(ROSA)26Sor⁺ Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺
Genetic Background	involves: 129S6/SvEvTac * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm4(EWSR1/ATF1)Mrc} mutation (0 available); any Gt(ROSA)26Sor mutation (993 available)
Pax7^{tm1(cre/ERT2)Gaka} mutation (1 available); any Pax7 mutation (38 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

muscle

myopathy ( J:194308 )

• when tamoxifen is administered after 3 weeks of age myopathy develops by 12 months

neoplasm

neoplasm ( J:194308 )

N	• no abnormal tumor development when treated with tamoxifen after 3 weeks of age

Allelic Composition	Six1^tm2.1Mair/Six1^tm2.1Mair Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺
Genetic Background	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6Cr * C57BL/6NTac

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax7^{tm1(cre/ERT2)Gaka} mutation (1 available); any Pax7 mutation (38 available)
Six1^tm2.1Mair mutation (0 available); any Six1 mutation (19 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

Perturbed myogenic differentiation of Six1^tm2.1Mair/Six1^tm2.1Mair Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺ satellite cell descendants ex vivo

muscle

muscle phenotype ( J:190460 )

N	• normal muscle tissue weights or histology up to a year after tamoxifen treatment

skeletal muscle fibrosis ( J:190460 )

• in tamoxifen-treated mice subjected to a single cardiotoxin injury

abnormal muscle physiology ( J:190460 )

• satellite cells from tamoxifen-treated mice exhibit reduced ability to differentiate of satellite cell descendants ex vivo and increased self-renewal capacity in vivo compared with control cells

• ex vivo satellite cells from tamoxifen-treated mice exhibit increased self-renewal compared with control cells

• however, satellite cells exhibit normal quiescence, activation and proliferation and polarity of division

abnormal muscle regeneration ( J:190460 )

• satellite cells from tamoxifen-treated mice form smaller myotubes containing fewer nuclei ex vivo compared with control cells

• regenerating muscle from tamoxifen-treated mice subjected to a single cardiotoxin injury exhibit smaller newly formed myofibers with fewer nuclei, numerous lagged fibers of small caliber, reduced cell size and necrotic myofibers compared with controls

Allelic Composition	Zfp422^em1Mode/Zfp422^em1Mode Pax7^{tm1(cre/ERT2)Gaka}/Pax7⁺
Genetic Background	involves: 129S6/SvEvTac * C57BL/6J * C57BL/6NCrl

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax7^{tm1(cre/ERT2)Gaka} mutation (1 available); any Pax7 mutation (38 available)
Zfp422^em1Mode mutation (0 available); any Zfp422 mutation (16 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

muscle

abnormal myoblast differentiation ( J:297035 )

• decrease in the number of myogenin positive cells in cultured satellite cells from tamoxifen treated mice indicating a defect in myoblast differentiation

• when induced to differentiate for 3 days satellite cells for fewer myotubes and have reduced fusion and differentiation indices

impaired skeletal muscle regeneration ( J:297035 )

• following CTX-induced muscle injury muscles in tamoxifen treated mice show smaller regenerated myofiber cross-section area

cellular

abnormal myoblast differentiation ( J:297035 )

• decrease in the number of myogenin positive cells in cultured satellite cells from tamoxifen treated mice indicating a defect in myoblast differentiation

• when induced to differentiate for 3 days satellite cells for fewer myotubes and have reduced fusion and differentiation indices

Allelic Composition	Pax7^{tm1(cre/ERT2)Gaka}/0 Snhg15^tm1.1Nju/Snhg15^tm1.1Nju
Genetic Background	involves: C57BL/6J * SJL/J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax7^{tm1(cre/ERT2)Gaka} mutation (1 available); any Pax7 mutation (38 available)
Snhg15^tm1.1Nju mutation (0 available); any Snhg15 mutation (9 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

muscle

delayed skeletal muscle regeneration ( J:291983 )

• delayed muscle regeneration after knockout induction by tamoxifen injection and BaCl₂ injection into tibialis anterior (TA) muscles at age 2 months: reduced number of embryonic myosin heavy chain expressing myofibers 4 days after BaCl₂ injection

• reduced myoblast proliferation after BaCl₂ injection

• reduced in vitro proliferation of stem cells after BaCl₂ injection

Allelic Composition	Gt(ROSA)26Sor^{tm1.1(DUX4*)Plj}/Gt(ROSA)26Sor⁺ Pax7^{tm1(cre/ERT2)Gaka}/?
Genetic Background	involves: 129S6/SvEvTac * C57BL/6 * C57BL/6NCrl

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sor^{tm1.1(DUX4*)Plj} mutation (1 available); any Gt(ROSA)26Sor mutation (993 available)
Pax7^{tm1(cre/ERT2)Gaka} mutation (1 available); any Pax7 mutation (38 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

normal phenotype

no abnormal phenotype detected ( J:256652 )

• mice are viable and healthy in the absence of tamoxifen

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