Phenotypes associated with this allele

• Allele Symbol: Cdk6^tm2.1Phin
• Allele Name: targeted mutation 2.1, Philip Hinds
• Allele ID: MGI:5141514
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cdk6^tm2.1Phin/Cdk6^tm2.1Phin	involves: 129 * C57BL/6 * FVB/N	MGI:5141601
cx2	Cdk6^tm2.1Phin/Cdk6^tm2.1Phin Il2ra^tm1Dw/Il2ra^tm1Dw	involves: 129S4/SvJae * C57BL/6 * FVB/N	MGI:5141604

Genotype
MGI:5141601

hm1

• Allelic Composition: Cdk6^tm2.1Phin/Cdk6^tm2.1Phin
• Genetic Background: involves: 129 * C57BL/6 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

small thymus ( J:174697 )

• compared with Cdk6^tm1.1Phin homozygotes

decreased thymocyte number ( J:174697 )

• 3-fold compared with Cdk6^tm1.1Phin homozygotes

decreased DN1 thymic pro-T cell number ( J:174697 )

• 2 times compared with Cdk6^tm1.1Phin homozygotes

decreased DN2 thymocyte number ( J:174697 )

• 2 times compared with Cdk6^tm1.1Phin homozygotes

decreased DN4 thymocyte number ( J:174697 )

• marginal compared with Cdk6^tm1.1Phin homozygotes

abnormal T cell differentiation ( J:174697 )

• fewer DN3 cells are in the S-G2-M phases of the cell cycle compared to in Cdk6^tm1.1Phin homozygotes

increased double-negative T cell number ( J:174697 )

• compared with Cdk6^tm1.1Phin homozygotes

increased DN3 thymocyte number ( J:174697 )

• 1.9-fold compared with Cdk6^tm1.1Phin homozygotes

decreased double-positive T cell number ( J:174697 )

• compared with Cdk6^tm1.1Phin homozygotes

increased CD4-positive, alpha-beta T cell number ( J:174697 )

• compared with Cdk6^tm1.1Phin homozygotes

increased CD8-positive, alpha-beta T cell number ( J:174697 )

• compared with Cdk6^tm1.1Phin homozygotes

decreased hematopoietic stem cell number ( J:174697 )

• 50% reduction in LSK cells compared with Cdk6^tm1.1Phin homozygotes

• one-third reduction in absolute number of LSK cells compared with Cdk6^tm1.1Phin homozygotes

abnormal hematopoietic stem cell physiology ( J:174697 )

• mice exhibit a 45% decrease in the fraction of cycling LSK cells compared with Cdk6^tm1.1Phin homozygotes

immune system

small thymus ( J:174697 )

• compared with Cdk6^tm1.1Phin homozygotes

decreased thymocyte number ( J:174697 )

• 3-fold compared with Cdk6^tm1.1Phin homozygotes

decreased DN1 thymic pro-T cell number ( J:174697 )

• 2 times compared with Cdk6^tm1.1Phin homozygotes

decreased DN2 thymocyte number ( J:174697 )

• 2 times compared with Cdk6^tm1.1Phin homozygotes

decreased DN4 thymocyte number ( J:174697 )

• marginal compared with Cdk6^tm1.1Phin homozygotes

abnormal T cell differentiation ( J:174697 )

• fewer DN3 cells are in the S-G2-M phases of the cell cycle compared to in Cdk6^tm1.1Phin homozygotes

increased double-negative T cell number ( J:174697 )

• compared with Cdk6^tm1.1Phin homozygotes

increased DN3 thymocyte number ( J:174697 )

• 1.9-fold compared with Cdk6^tm1.1Phin homozygotes

decreased double-positive T cell number ( J:174697 )

• compared with Cdk6^tm1.1Phin homozygotes

increased CD4-positive, alpha-beta T cell number ( J:174697 )

• compared with Cdk6^tm1.1Phin homozygotes

increased CD8-positive, alpha-beta T cell number ( J:174697 )

• compared with Cdk6^tm1.1Phin homozygotes

endocrine/exocrine glands

small thymus ( J:174697 )

• compared with Cdk6^tm1.1Phin homozygotes

decreased thymocyte number ( J:174697 )

• 3-fold compared with Cdk6^tm1.1Phin homozygotes

decreased DN1 thymic pro-T cell number ( J:174697 )

• 2 times compared with Cdk6^tm1.1Phin homozygotes

decreased DN2 thymocyte number ( J:174697 )

• 2 times compared with Cdk6^tm1.1Phin homozygotes

decreased DN4 thymocyte number ( J:174697 )

• marginal compared with Cdk6^tm1.1Phin homozygotes

increased DN3 thymocyte number ( J:174697 )

• 1.9-fold compared with Cdk6^tm1.1Phin homozygotes

Genotype
MGI:5141604

cx2

• Allelic Composition: Cdk6^tm2.1Phin/Cdk6^tm2.1Phin; Il2ra^tm1Dw/Il2ra^tm1Dw
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * FVB/N

hematopoietic system

hematopoietic system phenotype ( J:174697 )

N • mice exhibit normal thymocyte cellularity and accumulation in DN3 stage compared to in Cdk6^tm1.1Phin homozygotes

increased CD4-positive, alpha-beta T cell number ( J:174697 )

• compared to in Cdk6^tm1.1Phin homozygotes

increased CD8-positive, alpha-beta T cell number ( J:174697 )

• compared to in Cdk6^tm1.1Phin homozygotes

increased hematopoietic stem cell number ( J:174697 )

• compared with control and Cdk6^tm2.1Phin homozygotes

immune system

increased CD4-positive, alpha-beta T cell number ( J:174697 )

• compared to in Cdk6^tm1.1Phin homozygotes

increased CD8-positive, alpha-beta T cell number ( J:174697 )

• compared to in Cdk6^tm1.1Phin homozygotes