Phenotypes associated with this allele

• Allele Symbol: Fam76b^{Gt(IST14004H1)Tigm}
• Allele Name: gene trap IST14004H1, Texas A&M Institute for Genomic Medicine
• Allele ID: MGI:5203950
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fam76b^Gt(IST14004H1)Tigm/Fam76b^Gt(IST14004H1)Tigm	C57BL/6-Fam76b^Gt(IST14004H1)Tigm	MGI:7779984

Genotype
MGI:7779984

hm1

• Allelic Composition: Fam76b^{Gt(IST14004H1)Tigm}/Fam76b^{Gt(IST14004H1)Tigm}
• Genetic Background: C57BL/6-Fam76b^{Gt(IST14004H1)Tigm}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

enlarged spleen ( J:358021 )

• spleens are visibly enlarged at 5 months of age

increased spleen weight ( J:358021 )

• at 5 months of age, the spleen-to-body weight ratio is significantly higher than in wild-type controls

decreased B cell number ( J:358021 )

• at 5 months of age, spleens show a slight reduction in CD19+ B cells relative to wild-type spleens

microgliosis ( J:358021 )

• at 3 days after traumatic brain injury (TBI) by controlled cortical impact (CCI), the densities of IBA-1+ microglia are significantly higher in the hippocampus adjacent to the cortical contusion than in CCI-injured wild-type or sham controls

abnormal spleen white pulp morphology ( J:358021 )

• at 5 months of age, spleens show hypertrophy of the white pulp

• however, red pulp morphology is normal

abnormal spleen germinal center morphology ( J:358021 )

• following intraperitoneal (i.p.) injection of lipopolysaccharide (LPS), spleens show abundant tingible body macrophages in the germinal center, whereas no tingible body macrophages are detected in LPS-treated wild-type spleens

• however, PBS-treated mice show no significant morphological changes relative to PBS-treated wild-type controls

abnormal splenic cell ratio ( J:358021 )

• at 5 months of age, spleens show a significantly higher CD11b+ myeloid population and a slight reduction in CD19+ B cells relative to wild-type spleens

• however, no changes are detected in CD3+ T cells

abnormal macrophage physiology ( J:358021 )

• after i.p. LPS treatment, accumulation of tingible body macrophages in the spleen white pulp suggests that white pulp macrophages are impaired in their ability to clear out apoptotic cells produced during the germinal center reaction

increased microglial cell activation ( J:358021 )

• at 12 months, but not at 4 months, of age, mice show a significantly higher number of IBA-1+ microglial cells with highly reactive morphology and abundant cytoplasm in the hippocampus and thalamus than age-matched wild-type controls

• at 12 months, the densities (number/mm2) of IBA-1+ microglia in the CA1 region and thalamus are significantly higher than in wild-type controls, indicating enhanced microglial cell activation and neuroinflammation

increased interleukin-6 secretion ( J:358021 )

• after LPS stimulation, isolated bone marrow-derived macrophages (BMDMs) show significantly higher Il6 mRNA expression levels than LPS-treated wild-type BMDMs

• BMDMs from mice infected with a lentiviral vector expressing FAM76B show marked downregulation of Il6 mRNA expression in the presence of LPS

• at 3 days after traumatic brain injury (TBI) by controlled cortical impact (CCI), Il6 mRNA levels in the ipsilateral hippocampus are significantly higher than in CCI-injured wild-type controls

• 24 hr after LPS injection into the prefrontal cortex, both Il6 mRNA and protein IL-6 levels at the injection site are significantly higher than in LPS-injected wild-type controls

increased tumor necrosis factor secretion ( J:358021 )

• 24 hr after LPS injection into the prefrontal cortex, both Tnf mRNA and protein TNF levels at the injection site are significantly higher than in LPS-injected wild-type controls

increased inflammatory response ( J:358021 )

• at 7 days after LPS injection into the prefrontal cortex, brains show abundant macrophages at the injection site, whereas only a mild inflammatory response in seen in LPS-injected wild-type controls

hematopoietic system

enlarged spleen ( J:358021 )

• spleens are visibly enlarged at 5 months of age

increased spleen weight ( J:358021 )

• at 5 months of age, the spleen-to-body weight ratio is significantly higher than in wild-type controls

decreased B cell number ( J:358021 )

• at 5 months of age, spleens show a slight reduction in CD19+ B cells relative to wild-type spleens

increased myeloid cell number ( J:358021 )

• at 5 months of age, spleens show a significantly higher CD11b+ myeloid population than wild-type spleens

microgliosis ( J:358021 )

• at 3 days after traumatic brain injury (TBI) by controlled cortical impact (CCI), the densities of IBA-1+ microglia are significantly higher in the hippocampus adjacent to the cortical contusion than in CCI-injured wild-type or sham controls

abnormal spleen white pulp morphology ( J:358021 )

• at 5 months of age, spleens show hypertrophy of the white pulp

• however, red pulp morphology is normal

abnormal spleen germinal center morphology ( J:358021 )

• following intraperitoneal (i.p.) injection of lipopolysaccharide (LPS), spleens show abundant tingible body macrophages in the germinal center, whereas no tingible body macrophages are detected in LPS-treated wild-type spleens

• however, PBS-treated mice show no significant morphological changes relative to PBS-treated wild-type controls

abnormal splenic cell ratio ( J:358021 )

• at 5 months of age, spleens show a significantly higher CD11b+ myeloid population and a slight reduction in CD19+ B cells relative to wild-type spleens

• however, no changes are detected in CD3+ T cells

abnormal macrophage physiology ( J:358021 )

• after i.p. LPS treatment, accumulation of tingible body macrophages in the spleen white pulp suggests that white pulp macrophages are impaired in their ability to clear out apoptotic cells produced during the germinal center reaction

increased microglial cell activation ( J:358021 )

• at 12 months, but not at 4 months, of age, mice show a significantly higher number of IBA-1+ microglial cells with highly reactive morphology and abundant cytoplasm in the hippocampus and thalamus than age-matched wild-type controls

• at 12 months, the densities (number/mm2) of IBA-1+ microglia in the CA1 region and thalamus are significantly higher than in wild-type controls, indicating enhanced microglial cell activation and neuroinflammation

growth/size/body

decreased body weight ( J:358021 )

• average male body weight is significantly lower than that in wild-type controls from 30 to about 270 days of age

enlarged spleen ( J:358021 )

• spleens are visibly enlarged at 5 months of age

increased spleen weight ( J:358021 )

• at 5 months of age, the spleen-to-body weight ratio is significantly higher than in wild-type controls

nervous system

microgliosis ( J:358021 )

• at 3 days after traumatic brain injury (TBI) by controlled cortical impact (CCI), the densities of IBA-1+ microglia are significantly higher in the hippocampus adjacent to the cortical contusion than in CCI-injured wild-type or sham controls

increased microglial cell activation ( J:358021 )

• at 12 months, but not at 4 months, of age, mice show a significantly higher number of IBA-1+ microglial cells with highly reactive morphology and abundant cytoplasm in the hippocampus and thalamus than age-matched wild-type controls

• at 12 months, the densities (number/mm2) of IBA-1+ microglia in the CA1 region and thalamus are significantly higher than in wild-type controls, indicating enhanced microglial cell activation and neuroinflammation

homeostasis/metabolism

increased response to brain injury ( J:358021 )

• at 3 days after traumatic brain injury (TBI) by controlled cortical impact (CCI), mice show enhanced microglia activation and neuroinflammation, with more prominent increases in IBA-1+ microglia densities adjacent to the contusion site and higher Il6 mRNA levels in the ipsilateral hippocampus than CCI-injured wild-type controls