mortality/aging
• some die between E13.5 and E18.5
|
• some die between E11.5 and E13.5
|
• at E18.5 only 7% of mice are homozygous rather than the expected 25%
• death occurs between E11.5 and E18.5
|
reproductive system
N |
• males are fertile
|
|
• at P5 ovaries contained less than 20% as many oocytes as wild-type ovaries
|
|
• at E13.5 and P5
• however, gonocyte proliferation is seen at P5 and P10 in males
|
• at E11.5, most males and females have less than 5% as many germ cells as found in wild-type littermate controls in the genital ridge
• at E13.5 the number of MVH+ germ cells is reduced in males and females
|
• many mislocalized PGCs (located near the hindlimb buds and the base of the tail) are seen at E9.5
• ectopic PGCs are detected as early as E8.5 and as late as E11.5
|
|
• at 5-8 weeks of age a small number of follicles are occasionally seen
|
|
• ovaries display very few primordial follicles at P5 and P10
|
|
• ovaries of females older than 10 weeks of age lack follicular structures
|
small ovary
(
J:175228
)
|
• ovary volume is less than 20% that of wild-type controls in adult females
|
|
• empty tubules are seen alongside tubules filled with germ cells at various stages of spermatogenesis
|
embryo
• before E10.5 about 16% of embryos are located outside the yolk sac
|
• in embryos with developmental delay
|
• about 40% of embryos display general developmental delay by E8.5
|
• in embryos with developmental delay
|
• embryos with developmental delay have a reduced number of somites
|
• in embryos with developmental delay
|
nervous system
• in embryos with developmental delay
|
growth/size/body
• about 40% of embryos display general developmental delay by E8.5
|
endocrine/exocrine glands
|
• at 5-8 weeks of age a small number of follicles are occasionally seen
|
|
• ovaries display very few primordial follicles at P5 and P10
|
|
• ovaries of females older than 10 weeks of age lack follicular structures
|
small ovary
(
J:175228
)
|
• ovary volume is less than 20% that of wild-type controls in adult females
|
|
• empty tubules are seen alongside tubules filled with germ cells at various stages of spermatogenesis
|
cellular
|
• at P5 ovaries contained less than 20% as many oocytes as wild-type ovaries
|
|
• at E13.5 and P5
• however, gonocyte proliferation is seen at P5 and P10 in males
|
• at E11.5, most males and females have less than 5% as many germ cells as found in wild-type littermate controls in the genital ridge
• at E13.5 the number of MVH+ germ cells is reduced in males and females
|
• many mislocalized PGCs (located near the hindlimb buds and the base of the tail) are seen at E9.5
• ectopic PGCs are detected as early as E8.5 and as late as E11.5
|