Phenotypes associated with this allele

• Allele Symbol: Drd2^tm1.1Mrub
• Allele Name: targeted mutation 1.1, Marcelo Rubinstein
• Allele ID: MGI:5292350
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Drd2^tm1.1Mrub/Drd2^tm1.1Mrub Slc6a3^tm1.1(cre)Bkmn/Slc6a3^+	B6.Cg-Drd2^tm1.1Mrub Slc6a3^tm1.1(cre)Bkmn	MGI:5292386
cn2	Drd2^tm1.1Mrub/Drd2^tm1.1Mrub Tg(Prl-cre)1Mrub/?	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:5471747
cn3	Drd2^tm1.1Mrub/Drd2^tm1.1Mrub Tg(Nes-cre)1Kln/?	involves: 129S4/SvJae * C57BL/6 * SJL	MGI:5471750

Genotype
MGI:5292386

cn1

• Allelic Composition: Drd2^tm1.1Mrub/Drd2^tm1.1Mrub; Slc6a3^{tm1.1(cre)Bkmn}/Slc6a3⁺
• Genetic Background: B6.Cg-Drd2^tm1.1Mrub Slc6a3^{tm1.1(cre)Bkmn}

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

enhanced behavioral response to cocaine ( J:176167 )

• locomotor activity is increased 1.4-fold at low doses in cocaine-treated mice unlike in similarly treated control mice

• at higher doses, cocaine-induced hyperactivity is 3.8-fold compared to 2.8-fold in similarly treated control mice

abnormal operant conditioning behavior ( J:176167 )

• mice exhibit increased motivation to work for food compared with control mice

• however, mice exhibit normal profile of loss of responding following cessation of reward delivery

hyperactivity ( J:176167 )

• in an open field that is insensitive to quinpirole treatment

• however, habituation is normal

induced hyperactivity ( J:176167 )

• locomotor activity is increased 1.4-fold at low doses in cocaine-treated mice unlike in similarly treated control mice

• at higher doses, cocaine-induced hyperactivity is 3.8-fold compared to 2.8-fold in similarly treated control mice

nervous system

abnormal single cell response ( J:176167 )

• quinpirole fails to induce a slow hyperpolarizing current in dopaminergic neurons unlike in similarly treated control cells

• however, response to baclofen is normal

abnormal inhibitory postsynaptic currents ( J:176167 )

• decreased and insensitive to sulpiride

abnormal synaptic dopamine release ( J:176167 )

• during train stimulation, mice exhibit supramaximal dopamine release that increases during stimulation that is insensitive to sulpiride unlike in control mice

homeostasis/metabolism

decreased physiological sensitivity to xenobiotic ( J:176167 )

• quinpirole fails to induce a slow hyperpolarizing current in dopaminergic neurons or reduce locomotor activity unlike in similarly treated control mice

• sulpiride fails to decreased inhibitory postsynaptic currents unlike in control cells

• tyrosine hydroxylase activity is insensitive to quinpirole unlike in control cells

• however, response to baclofen is normal

increased physiological sensitivity to xenobiotic ( J:176167 )

• mice treated with haloperidol exhibit greater decrease in locomotion compared with similarly treated control mice

Genotype
MGI:5471747

cn2

• Allelic Composition: Drd2^tm1.1Mrub/Drd2^tm1.1Mrub; Tg(Prl-cre)1Mrub/?
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CBA

growth/size/body

growth/size/body region phenotype ( J:194227 )

♂N • body weight and body size are normal in males

homeostasis/metabolism

increased circulating prolactin level ( J:194227 )

• hyperprolactinemia in both sexes but particularly in females

decreased physiological sensitivity to xenobiotic ( J:194227 )

• haloperidol fails to increase serum prolactin in either sex as it does in controls

• haloperidol induces catatonia as in controls

Genotype
MGI:5471750

cn3

• Allelic Composition: Drd2^tm1.1Mrub/Drd2^tm1.1Mrub; Tg(Nes-cre)1Kln/?
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * SJL

growth/size/body

postnatal growth retardation ( J:194227 )

♂ • reduced body weight and body length in males

♂ • body length at weaning is reduced 10.2% in males

slow postnatal weight gain ( J:194227 )

♂ • body weight of males is 14.2% lower than controls at 2 months and 21.7% lower at 6 months

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:194227 )

N • prolactin levels are normal

abnormal hormone level ( J:194227 )

• growth hormone releasing hormone (GHRH) levels are reduced 30%

decreased circulating insulin-like growth factor I level ( J:194227 )

• serum levels are reduced 18.7%

decreased growth hormone level ( J:194227 )

• 63.2% reduction in growth hormone in the pituitary

decreased urine major urinary protein level ( J:194227 )

♂ • 45.6% reduction in MUP levels

♂ • male MUP levels are similar to female control levels

decreased physiological sensitivity to xenobiotic ( J:194227 )

• haloperidol fails to induce catatonia

behavior/neurological

behavior/neurological phenotype ( J:194227 )

N • food intake is normal

abnormal social/conspecific interaction behavior ( J:194227 )

♂ • in social dominance tests control C57BL/6 males respond to mutant male mice as if they were females - no social dominance

♂ • resident male C57BL/6 confronted with castrated males swabbed with urine from mutant mice showed reduced aggression

♂ • reduced scent marking of C57NL/6 male mice when placed in cages previously occupied by mutant mice

endocrine/exocrine glands

decreased somatotroph cell number ( J:194227 )

• 28.1% reduction in the number of growth hormone containing cells in the pituitary

renal/urinary system

decreased urine major urinary protein level ( J:194227 )

♂ • 45.6% reduction in MUP levels

♂ • male MUP levels are similar to female control levels

nervous system

decreased somatotroph cell number ( J:194227 )

• 28.1% reduction in the number of growth hormone containing cells in the pituitary