Phenotypes associated with this allele

• Allele Symbol: Tg(CAG-mRFP1,-SOX9,-EGFP)1Haak
• Allele Name: transgene insertion 1, Haruhiko Akiyama
• Allele ID: MGI:5293615
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Col10a1^tm1(cre)Haak/Col10a1^+ Tg(CAG-mRFP1,-SOX9,-EGFP)1Haak/0	involves: 129S6/SvEvTac * C57BL/6	MGI:5297173
cn2	Tg(CAG-mRFP1,-SOX9,-EGFP)1Haak/0 Tg(Col2a1-cre)1Bhr/0	involves: C57BL/6 * SJL	MGI:5297172

Genotype
MGI:5297173

cn1

• Allelic Composition: Col10a1^tm1(cre)Haak/Col10a1⁺; Tg(CAG-mRFP1,-SOX9,-EGFP)1Haak/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

abnormal bone mineralization ( J:176952 )

• mineralization eventually occurs, but mineralized domain is markedly reduced compared to controls

abnormal endochondral bone ossification ( J:176952 )

• endochondral bone formation is impaired

abnormal chondrocyte physiology ( J:176952 )

• terminal differentiation of hypertrophic chondrocytes is delayed as is subsequent cartilage replacement by bone

Genotype
MGI:5297172

cn2

• Allelic Composition: Tg(CAG-mRFP1,-SOX9,-EGFP)1Haak/0; Tg(Col2a1-cre)1Bhr/0
• Genetic Background: involves: C57BL/6 * SJL

skeleton

abnormal endochondral bone ossification ( J:176952 )

• delay in formation of primary ossification centers in forelimb skeletal elements is observed in E17.5 embryos

abnormal chondrocyte physiology ( J:176952 )

• delayed hypertrophic conversion of proliferating chondrocytes is seen in E15.5 embryos

limbs/digits/tail

abnormal forelimb morphology ( J:176952 )

• an expansion of the hypertrophic zone of in forelimb skeletal elements in observed in E17.5 embryos