Phenotypes associated with this allele

• Allele Symbol: Col1a1^{tm2(tetO-LIN28B)Gqda}
• Allele Name: targeted mutation 2, George Q Daley
• Allele ID: MGI:5294612
• Summary: 7 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Col1a1^tm2(tetO-LIN28B)Gqda/Col1a1^+ Foxd1^tm1(GFP/cre)Amc/Foxd1^+ Gt(ROSA)26Sor^tm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor^+	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6	MGI:5638869
cn2	Col1a1^tm2(tetO-LIN28B)Gqda/Col1a1^tm3(tetO-Mirlet7g/Mir21)Gqda Gt(ROSA)26Sor^tm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor^+ Wt1^tm2(cre/ERT2)Wtp/Wt1^+	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6	MGI:5638880
cn3	Col1a1^tm2(tetO-LIN28B)Gqda/Col1a1^+ Gt(ROSA)26Sor^tm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor^+ Wt1^tm2(cre/ERT2)Wtp/Wt1^+	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6	MGI:5638793
cn4	Col1a1^tm2(tetO-LIN28B)Gqda/Col1a1^+ Gt(ROSA)26Sor^tm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor^+ Tg(Six2-EGFP/cre)1Amc/0	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6 * CD-1	MGI:5638867
cn5	Col1a1^tm2(tetO-LIN28B)Gqda/Col1a1^+ Gt(ROSA)26Sor^tm1(rtTA,EGFP)Nagy/Gt(ROSA)26Sor^+ Tg(Cdh16-cre)91Igr/0	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6 * ICR	MGI:5638874
cx6	Col1a1^tm2(tetO-LIN28B)Gqda/Col1a1^+ Gt(ROSA)26Sor^tm1(rtTA*M2)Jae/Gt(ROSA)26Sor^+	involves: 129S4/SvJae * C57BL/6 * CD-1	MGI:5294614
cx7	Col1a1^tm2(tetO-LIN28B)Gqda/Col1a1^tm3(tetO-Mirlet7g/Mir21)Gqda Gt(ROSA)26Sor^tm1(rtTA*M2)Jae/Gt(ROSA)26Sor^+	involves: 129S4/SvJae * C57BL/6 * CD-1	MGI:5294616

Genotype
MGI:5638869

cn1

• Allelic Composition: Col1a1^{tm2(tetO-LIN28B)Gqda}/Col1a1⁺; Foxd1^{tm1(GFP/cre)Amc}/Foxd1⁺; Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy}/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

renal/urinary system

hydronephrosis ( J:211179 )

• mice develop hydronephrosis following doxycycline induction

neoplasm

neoplasm ( J:211179 )

N • mice do not develop renal tumors following doxycycline induction

Genotype
MGI:5638880

cn2

• Allelic Composition: Col1a1^{tm2(tetO-LIN28B)Gqda}/Col1a1^{tm3(tetO-Mirlet7g/Mir21)Gqda}; Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy}/Gt(ROSA)26Sor⁺; Wt1^{tm2(cre/ERT2)Wtp}/Wt1⁺
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6

renal/urinary system

small kidney ( J:211179 )

• small kidney in mice induced with doxycycline from E14.5 until the end of the experiment

• however, cap mesenchyme differentiates normally in doxycycline induced mutants

Genotype
MGI:5638793

cn3

• Allelic Composition: Col1a1^{tm2(tetO-LIN28B)Gqda}/Col1a1⁺; Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy}/Gt(ROSA)26Sor⁺; Wt1^{tm2(cre/ERT2)Wtp}/Wt1⁺
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6

neoplasm

increased kidney tumor incidence ( J:211179 )

• all mice develop kidney tumors within the first 2 weeks of life following doxycycline (Dox) induction during embryonic development at E0, E14.5 or E18.5

• tumors resemble Wilms tumor

• mice treated with doxycycline at P10 do not develop tumors

renal/urinary system

kidney cyst ( J:211179 )

• mice treated with doxycycline at P10 develop cystic kidneys

increased kidney tumor incidence ( J:211179 )

• all mice develop kidney tumors within the first 2 weeks of life following doxycycline (Dox) induction during embryonic development at E0, E14.5 or E18.5

• tumors resemble Wilms tumor

• mice treated with doxycycline at P10 do not develop tumors

abnormal kidney mesenchyme morphology ( J:211179 )

• doxycycline induced mice exhibit persistent proliferation of cap mesenchyme cells in adults

• however, cap mesenchyme cells within tumors retain a differentiation capacity that recapitulates normal kidney development

delayed kidney development ( J:211179 )

• timing of kidney development is prolonged in doxycycline induced mice, with sustaining proliferation of the cap mesenchyme cells into adulthood

growth/size/body

kidney cyst ( J:211179 )

• mice treated with doxycycline at P10 develop cystic kidneys

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
nephroblastoma		DOID:2154	OMIM:194070	J:211179

Genotype
MGI:5638867

cn4

• Allelic Composition: Col1a1^{tm2(tetO-LIN28B)Gqda}/Col1a1⁺; Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy}/Gt(ROSA)26Sor⁺; Tg(Six2-EGFP/cre)1Amc/0
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6 * CD-1

renal/urinary system

kidney cyst ( J:211179 )

• mice develop cystic kidneys when transgene expression is induced with doxycycline early in embryonic development or in adult mice

neoplasm

neoplasm ( J:211179 )

N • mice do not develop renal tumors following doxycycline induction

growth/size/body

kidney cyst ( J:211179 )

• mice develop cystic kidneys when transgene expression is induced with doxycycline early in embryonic development or in adult mice

Genotype
MGI:5638874

cn5

• Allelic Composition: Col1a1^{tm2(tetO-LIN28B)Gqda}/Col1a1⁺; Gt(ROSA)26Sor^{tm1(rtTA,EGFP)Nagy}/Gt(ROSA)26Sor⁺; Tg(Cdh16-cre)91Igr/0
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6 * ICR

renal/urinary system

renal/urinary system phenotype ( J:211179 )

N • no kidney pathology is seen in mice induced with doxycycline

Genotype
MGI:5294614

cx6

• Allelic Composition: Col1a1^{tm2(tetO-LIN28B)Gqda}/Col1a1⁺; Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CD-1

growth/size/body

decreased susceptibility to diet-induced obesity ( J:177113 )

• following doxycycline treatment mice gain less weight when fed a high fat diet compared to wild-type mice

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:177113 )

• following doxycycline treatment mice gain less weight when fed a high fat diet compared to wild-type mice

hypoglycemia ( J:177113 )

• following doxycycline treatment average fasting glucose is less than 50 mg/dL

improved glucose tolerance ( J:177113 )

• following doxycycline treatment on a normal or high fat diet

increased insulin sensitivity ( J:177113 )

• following doxycycline treatment

Genotype
MGI:5294616

cx7

• Allelic Composition: Col1a1^{tm2(tetO-LIN28B)Gqda}/Col1a1^{tm3(tetO-Mirlet7g/Mir21)Gqda}; Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * CD-1

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:177113 )

N • following doxycycline treatment responses in a glucose tolerance test are similar to controls