Phenotypes associated with this allele

• Allele Symbol: Usp2^tm1.1Ssw
• Allele Name: targeted mutation 1.1, Simon S Wing
• Allele ID: MGI:5295262
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Usp2^tm1.1Ssw/Usp2^tm1.1Ssw	involves: C57BL/6	MGI:5295264

Genotype
MGI:5295264

hm1

• Allelic Composition: Usp2^tm1.1Ssw/Usp2^tm1.1Ssw
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal male germ cell morphology ( J:176705 )

♂ • spermatozoa in the lumen of the seminiferous tubules show an increase in the presence of multinucleated bodies

abnormal sperm motility ( J:176705 )

♂ • when transferred to media (PBS) deficient in nutrients and ions mutant sperm rapidly lose motility, with over 95% of sperm being immotile within 30 minutes, unlike wild-type spermatozoa

reproductive system

reproductive system phenotype ( J:176705 )

N • no defect is detected in female fertility

N • no decrease in sperm number and no defect in the acrosome reaction are detected

abnormal male germ cell morphology ( J:176705 )

♂ • spermatozoa in the lumen of the seminiferous tubules show an increase in the presence of multinucleated bodies

abnormal sperm motility ( J:176705 )

♂ • when transferred to media (PBS) deficient in nutrients and ions mutant sperm rapidly lose motility, with over 95% of sperm being immotile within 30 minutes, unlike wild-type spermatozoa

abnormal seminiferous tubule morphology ( J:176705 )

♂ • evidence for abnormal aggregation of elongating spermatids and formation of multinucleated cells is seen in some seminiferous tubules

decreased litter size ( J:176705 )

• average litter size for litters sired by mutant males is less than 50% that of litters sired by wild-type males

reduced male fertility ( J:176705 )

♂ • males rarely sire litters and litters that are produced are smaller in size

♂ • overall progeny generated by males is reduce 88-98% compared to wild-type males

impaired fertilization ( J:176705 )

• less than 10% of the eggs from a super ovulated wild-type female are fertilized following mating with mutant males

• about 10% of eggs are fertilized in vitro using sperm from mutant males compared to about 60% fertilization using sperm from wild-type males

• using ICSI or zona pellucida-free eggs, mutant sperm are able to activate eggs to begin embryonic development similar to wild -type sperm

behavior/neurological

behavior/neurological phenotype ( J:176705 )

N • no defect is detected in male copulatory behavior

endocrine/exocrine glands

abnormal seminiferous tubule morphology ( J:176705 )

♂ • evidence for abnormal aggregation of elongating spermatids and formation of multinucleated cells is seen in some seminiferous tubules