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Allele Symbol Allele Name Allele ID |
Gt(ROSA)26Sortm3(tetO-EGFP,-RNAi:T)Bgh targeted mutation 3, Bernhard G Herrmann MGI:5297100 |
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| Summary |
2 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• formation arrested at the level of the forelimbs when foster mothers treated with doxycycline
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• tail outgrowth fails or is abnormal when foster mothers treated with doxycycline
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice from pregnant females treated with doxycycline that survive to birth die within a few hours after birth due to respiratory distress
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• embryos from pregnant females treated with doxycycline exhibit increased mortality after E12.5, with only 35% surviving past E13.5
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• embryos from pregnant females treated with doxycycline develop severe caudal defects posterior to the hindlimb by E12.5 and exhibit caudal regression
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• caudal somites are absent at E12.5 in embryos from pregnant females treated with doxycycline but they are present at E10.5, indicating loss of mesodermal tissue between E10.5 and E12.5
• embryos from pregnant females treated with doxycycline exhibit unstructured mesodermal tissue posterior to the hindlimb bud by E12.5
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• severe reduction in paraxial mesenchyme in the area surrounding the notochord, located between the cloaca and neural tube, in E10.5 embryos from pregnant females treated with doxycycline
• E12.5 embryos from pregnant females treated with doxycycline exhibit increased cell death in caudal mesenchyme
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• embryos from pregnant females treated with doxycycline exhibit a wide but covered opening it the caudal neural tube by E12.5-E13.5
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• mice from pregnant females treated with doxycycline that survive to birth exhibit spina bifida occulta
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• embryos from pregnant females treated with doxycycline exhibit a kinked neural tube located at the level between the fore and hindlimb buds at E10.5
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• notochord structure is undifferentiated in E10.5 embryos from pregnant females treated with doxycycline, appearing larger and more tubular in structure rather than the compact morphology seen in controls and marker analysis indicates it adopts a neural character
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• no detectable notochord is seen at the level of the hindlimb in E12.5 embryos from pregnant females treated with doxycycline, indicating that the undifferentiated notochord seen at E12.5 degenerates by E12.5
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• embryos from pregnant females treated with doxycycline exhibit notochord arrest near the forelimb level at E9.5-E10.5, however they have a normal number of somites at this stage
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• disrupted somites are seen in E12.5 embryos from pregnant females treated with doxycycline
• caudal somites are absent at E12.5 in embryos from pregnant females treated with doxycycline but they are present at E10.5, indicating loss of mesodermal tissue/degeneration of somites between E10.5 and E12.5
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• embryos from pregnant females treated with doxycycline exhibit a wide but covered opening it the caudal neural tube by E12.5-E13.5
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• mice from pregnant females treated with doxycycline that survive to birth exhibit spina bifida occulta
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• embryos from pregnant females treated with doxycycline exhibit a kinked neural tube located at the level between the fore and hindlimb buds at E10.5
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• mutants from pregnant females treated with doxycycline exhibit various anorectal defects
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• seen in E13.5-P0 mutants from pregnant females treated with doxycycline
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• seen in E13.5-P0 mutants from pregnant females treated with doxycycline
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• seen in E13.5-P0 mutants from pregnant females treated with doxycycline
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• seen in E13.5-P0 mutants from pregnant females treated with doxycycline
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• embryos from pregnant females treated with doxycycline exhibit a rudimentary tail between E10.5 and E11.5
• mice from pregnant females treated with doxycycline that survive to birth exhibit a tail filament
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• majority of E18.5 mutants from pregnant females treated with doxycycline have no caudal vertebrae and the remaining have only a few, malformed caudal vertebrae with an absence of sacral vertebrae
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• 30% incidence of C7 to T1 homeotic transformation in E18.5 mutants from pregnant females treated with doxycycline
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• mutants from pregnant females treated with doxycycline exhibit various anorectal defects
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• seen in E13.5-P0 mutants from pregnant females treated with doxycycline
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• seen in one mutant from a pregnant female treated with doxycycline
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• surviving mutants from pregnant females treated with doxycycline exhibit respiratory distress at birth
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• in 6 of 9 mutants from pregnant females treated with doxycycline, the vertebral column is generally arrested at the lumbar/sacral level with several poorly developed lumbar vertebrae and a sacral rudiment at E18.5
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• mice from pregnant females treated with doxycycline that survive to birth exhibit spina bifida occulta
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• majority of E18.5 mutants from pregnant females treated with doxycycline have no caudal vertebrae and the remaining have only a few, malformed caudal vertebrae with an absence of sacral vertebrae
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• 30% incidence of C7 to T1 homeotic transformation in E18.5 mutants from pregnant females treated with doxycycline
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• mutants from pregnant females treated with doxycycline exhibit poorly developed lumbar vertebrae at E18.5
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• mutants from pregnant females treated with doxycycline exhibit only a sacral rudiment or absence of sacral vertebrae at E18.5
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 08/02/2024 MGI 6.24 |
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