Phenotypes associated with this allele

• Allele Symbol: Gdpd5^tm1.1Itl
• Allele Name: targeted mutation 1.1, inGenious Targeting Laboratory
• Allele ID: MGI:5302017
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gdpd5^tm1.1Itl/Gdpd5^tm1.1Itl	involves: 129 * C57BL/6J	MGI:5302060
cn2	Gdpd5^tm1Itl/Gdpd5^tm1.1Itl Gt(ROSA)26Sor^tm1(cre/ERT)Nat/Gt(ROSA)26Sor^+	involves: 129S1/Sv * 129/Sv * 129X1/SvJ * C57BL/6J	MGI:5302059
cx3	Gdpd5^tm1.1Itl/Gdpd5^tm1.1Itl Tg(Hlxb9-GFP)1Tmj/0	involves: 129/Sv * C57BL/6 * C57BL/6J * CBA	MGI:5302061

Genotype
MGI:5302060

hm1

• Allelic Composition: Gdpd5^tm1.1Itl/Gdpd5^tm1.1Itl
• Genetic Background: involves: 129 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal neuron differentiation ( J:178550 )

• mice exhibit reduced formation of motor neurons compared with wild-type mice

• at P5 and P28, alpha motor neuron differentiation is impaired in the lateral motor neurons

• however, no increase in apoptosis is observed and lateral motor column gamma motor neurons and medial motor column alpha and gamma motor neurons are intact

abnormal neuronal precursor proliferation ( J:178550 )

• fewer motor neuron progenitors exit the cell cycle and exhibit extended cell cycle compared to in wild-type mice

decreased motor neuron number ( J:178550 )

• at E11.5 and E13.5 in lateral motor neurons

• at E12.5, but not E13.5, in the lateral motor neurons of the adductor longus and magnus and posterior gracilis muscles

• at E13.5 in hypaxial motor neurons

• at E13.5 in the rectofemoratibialis muscles

• at E13.5 and E14.5 in the medial anterior gracilis, medial adductor brevis, and vasti muscles

• however, the number of medial motor neurons or preganglionic columns are normal

cellular

abnormal neuron differentiation ( J:178550 )

• mice exhibit reduced formation of motor neurons compared with wild-type mice

• at P5 and P28, alpha motor neuron differentiation is impaired in the lateral motor neurons

• however, no increase in apoptosis is observed and lateral motor column gamma motor neurons and medial motor column alpha and gamma motor neurons are intact

abnormal neuronal precursor proliferation ( J:178550 )

• fewer motor neuron progenitors exit the cell cycle and exhibit extended cell cycle compared to in wild-type mice

Genotype
MGI:5302059

cn2

• Allelic Composition: Gdpd5^tm1Itl/Gdpd5^tm1.1Itl; Gt(ROSA)26Sor^{tm1(cre/ERT)Nat}/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129S1/Sv * 129/Sv * 129X1/SvJ * C57BL/6J

nervous system

nervous system phenotype ( J:178550 )

N • mice treated with tamoxifen after neurogenesis (at E10.5) exhibit normal motor pool formation

abnormal neuron differentiation ( J:178550 )

• mice treated with tamoxifen at E8.5 exhibit delayed motor neuron formation compared with control mice

decreased motor neuron number ( J:178550 )

• at E12.5, mice treated with tamoxifen at E8.5 exhibit fewer Isl1/2+ and vasti, adductor longus and magnus, and posterior gracilis muscle motor neurons compared with control mice

• at E14.5, mice treated with tamoxifen at E8.5 exhibit fewer Isl1/2+ and vasti muscle motor neurons compared with control mice

• however, motor neurons in adductor longus and magnus and posterior gracilis muscles recover by E14.5

cellular

abnormal neuron differentiation ( J:178550 )

• mice treated with tamoxifen at E8.5 exhibit delayed motor neuron formation compared with control mice

Genotype
MGI:5302061

cx3

• Allelic Composition: Gdpd5^tm1.1Itl/Gdpd5^tm1.1Itl; Tg(Hlxb9-GFP)1Tmj/0
• Genetic Background: involves: 129/Sv * C57BL/6 * C57BL/6J * CBA

nervous system

abnormal motor neuron morphology ( J:178550 )

• mice exhibit thinner major axonal tracts emerging from lumbosacral segment 2 compared with wild-type mice

• however, existing lateral motor neurons at E12.5 and E14.5 are normal

decreased motor neuron number ( J:178550 )

• mice exhibit a delay and loss of lumbosacral segment motor neuron pools compared with wild-type mice