Phenotypes associated with this allele

• Allele Symbol: Pclaf^tm1.1Lkd
• Allele Name: targeted mutation 1.1, Lisa K Denzin
• Allele ID: MGI:5302414
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pclaf^tm1.1Lkd/Pclaf^tm1.1Lkd	involves: C57BL/6	MGI:5302416
cx2	Pclaf^tm1.1Lkd/Pclaf^tm1.1Lkd Trp53^tm1Tyj/Trp53^+	involves: 129S2/SvPas * C57BL/6	MGI:5302543

Genotype
MGI:5302416

hm1

• Allelic Composition: Pclaf^tm1.1Lkd/Pclaf^tm1.1Lkd
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

thymus hypoplasia ( J:177782 )

decreased common myeloid progenitor cell number ( J:177782 )

• CMPs reduced 2.2-fold

• GMPs reduced 1.6-fold

decreased bone marrow cell number ( J:177782 )

• more severe in older mice

abnormal common lymphocyte progenitor cell morphology ( J:177782 )

• reduced CLP numbers

decreased leukocyte cell number ( J:177782 )

decreased hematopoietic stem cell number ( J:177782 )

• 2- to 8-fold decrease in LSK hematopoietic stem cells

• reduction in the absolute numbers of multipotent progenitors (4.1-fold) and lymphoid-primed multipotent progenitors (10.2-fold)

spleen hypoplasia ( J:177782 )

abnormal hematopoietic system physiology ( J:177782 )

• apoptosis of lymphoid-primed multipotent progenitors (1.7-fold) and common lymphoid progenitors (2.5-fold) is increased compared to in control mice

• in competitive repopulation assays, mice exhibit reduced long term hematopoietic stem cells and impaired hematopoietic stem cell progenitor development compared with control mice

• after second transplant, bone marrow fails to rescue lethally irradiated recipients compared with wild-type bone marrow

abnormal hematopoietic stem cell physiology ( J:177782 )

• proliferation of long term hematopoietic stem cells is increased while quiescence is decreased compared to in control mice

cellular

oxidative stress ( J:177782 )

• long term hematopoietic stem cells exhibit increased reactive oxygen species compared with control cells

immune system

thymus hypoplasia ( J:177782 )

decreased leukocyte cell number ( J:177782 )

spleen hypoplasia ( J:177782 )

endocrine/exocrine glands

thymus hypoplasia ( J:177782 )

Genotype
MGI:5302543

cx2

• Allelic Composition: Pclaf^tm1.1Lkd/Pclaf^tm1.1Lkd; Trp53^tm1Tyj/Trp53⁺
• Genetic Background: involves: 129S2/SvPas * C57BL/6

hematopoietic system

increased hematopoietic stem cell number ( J:177782 )

• lymphoid-primed multipotent progenitors are increased compared to in 2810417H13Rik^tm1.1Lkd homozygotes