Phenotypes associated with this allele

• Allele Symbol: Gak^tm2Legr
• Allele Name: targeted mutation 2, Lois E Greene
• Allele ID: MGI:5305733
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gak^tm2Legr/Gak^tm2Legr	involves: 129S6/SvEvTac * C57BL/6	MGI:5305776
cn2	Gak^tm2Legr/Gak^tm2Legr	involves: 129S6/SvEvTac * C57BL/6	MGI:5305781
cn3	Gak^tm2Legr/Gak^tm2Legr Tg(KRT14-cre)1Amc/0	involves: 129S6/SvEvTac * C57BL/6 * CBA	MGI:5305779
cn4	Gak^tm2Legr/Gak^tm2Legr Tg(CAG-cre/Esr1*)5Amc/0	involves: 129S6/SvEvTac * C57BL/6 * CBA	MGI:5305780
cn5	Gak^tm2Legr/Gak^tm2Legr Speer6-ps1^Tg(Alb-cre)21Mgn/Speer6-ps1^+	involves: 129S6/SvEvTac * C57BL/6 * DBA	MGI:5305778
cn6	Gak^tm2Legr/Gak^tm2Legr Tg(Nes-cre)1Kln/0	involves: 129S6/SvEvTac * C57BL/6 * SJL	MGI:5305777

Genotype
MGI:5305776

hm1

• Allelic Composition: Gak^tm2Legr/Gak^tm2Legr
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:180119 )

• mice exhibit no phenotypic differences compared with wild-type mice

Genotype
MGI:5305781

cn2

• Allelic Composition: Gak^tm2Legr/Gak^tm2Legr
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

cellular

abnormal plasma membrane morphology ( J:180119 )

• mouse embryonic fibroblasts treated with a cre-expressing adenovirus exhibit altered clatherin-coated pits compared with control cells

Genotype
MGI:5305779

cn3

• Allelic Composition: Gak^tm2Legr/Gak^tm2Legr; Tg(KRT14-cre)1Amc/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CBA

mortality/aging

postnatal lethality, complete penetrance ( J:180119 )

• mice die shortly after birth

integument

impaired skin barrier function ( J:180119 )

• at E17.5

abnormal epidermal layer morphology ( J:180119 )

• the epidermis lacks extensive differentiation and multiple cell layers compared with control mice

• the epidermis exhibits reduced neutral lipids compared with control mice

abnormal epidermis stratum corneum morphology ( J:180119 )

• underdeveloped

wrinkled skin ( J:180119 )

• shortly after birth

homeostasis/metabolism

dehydration ( J:180119 )

• shortly after birth

impaired skin barrier function ( J:180119 )

• at E17.5

Genotype
MGI:5305780

cn4

• Allelic Composition: Gak^tm2Legr/Gak^tm2Legr; Tg(CAG-cre/Esr1*)5Amc/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * CBA

mortality/aging

premature death ( J:180119 )

• mice die within six days of tamoxifen treatment

Genotype
MGI:5305778

cn5

• Allelic Composition: Gak^tm2Legr/Gak^tm2Legr; Speer6-ps1^{Tg(Alb-cre)21Mgn}/Speer6-ps1⁺
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA

mortality/aging

postnatal lethality, complete penetrance ( J:180119 )

• by P5

liver/biliary system

abnormal bile duct morphology ( J:180119 )

• not well organized

abnormal hepatoblast differentiation ( J:180119 )

• hepatoblasts fail to differentiate

abnormal liver morphology ( J:180119 )

• yellowish liver

decreased liver glycogen level ( J:180119 )

• mice fail to accumulate glycogen unlike control mice

jaundice ( J:180119 )

• at P1 or P2

growth/size/body

increased body weight ( J:180119 )

• small during the first 4 days after birth

decreased body size ( J:180119 )

• at P1 or P2

homeostasis/metabolism

decreased liver glycogen level ( J:180119 )

• mice fail to accumulate glycogen unlike control mice

endocrine/exocrine glands

abnormal bile duct morphology ( J:180119 )

• not well organized

Genotype
MGI:5305777

cn6

• Allelic Composition: Gak^tm2Legr/Gak^tm2Legr; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * SJL

mortality/aging

postnatal lethality, complete penetrance ( J:180119 )

• mice die between P3 and P21

nervous system

abnormal neuron differentiation ( J:180119 )

• mice exhibit decreased numbers of neuronal precursors due to reduced proliferation and migration compared with control mice

abnormal neuronal precursor proliferation ( J:180119 )

• reduced

abnormal cortical ventricular zone morphology ( J:180119 )

• at E15.5, cellular density in the forebrain and hindbrain cortex is reduced compared to in control mice

• at P1, mice exhibit reduced cellularity and thickness of the cerebral cortex compared with control mice

abnormal Ammon gyrus morphology ( J:180119 )

• decreased cell density

abnormal dentate gyrus morphology ( J:180119 )

• decreased cell density

decreased neuronal precursor cell number ( J:180119 )

• due to reduced proliferation and migration

behavior/neurological

lethargy ( J:180119 )

• some mice are sluggish at P3

cellular

abnormal neuron differentiation ( J:180119 )

• mice exhibit decreased numbers of neuronal precursors due to reduced proliferation and migration compared with control mice

abnormal neuronal precursor proliferation ( J:180119 )

• reduced