Phenotypes associated with this allele

• Allele Symbol: Tg(Ins2-crmA)27Ngkh
• Allele Name: transgene insertion 27, Nicolas Glaichenhaus
• Allele ID: MGI:5308875
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(Ins2-crmA)27Ngkh/Tg(Ins2-crmA)27Ngkh	C.NOD-Tg(Ins2-CrmA)27Ngkh	MGI:5309113
tg2	Tg(Ins2-crmA)27Ngkh/0	C.NOD-Tg(Ins2-CrmA)27Ngkh	MGI:5309115
tg3	Tg(Ins2-crmA)27Ngkh/?	NOD-Tg(Ins2-CrmA)27Ngkh	MGI:5309128

Genotype
MGI:5309113

tg1

• Allelic Composition: Tg(Ins2-crmA)27Ngkh/Tg(Ins2-crmA)27Ngkh
• Genetic Background: C.NOD-Tg(Ins2-CrmA)27Ngkh

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality, complete penetrance ( J:82809 )

• this strain is "maintained in an heterozygous state since homozygous mice are not viable"

Genotype
MGI:5309115

tg2

• Allelic Composition: Tg(Ins2-crmA)27Ngkh/0
• Genetic Background: C.NOD-Tg(Ins2-CrmA)27Ngkh

normal phenotype

no abnormal phenotype detected ( J:82809 )

• no defects noted

Genotype
MGI:5309128

tg3

• Allelic Composition: Tg(Ins2-crmA)27Ngkh/?
• Genetic Background: NOD-Tg(Ins2-CrmA)27Ngkh

cellular

decreased sensitivity to induced cell death ( J:132052 )

• after 60 hours' incubation of pancreatic islets from transgenic or wild-type NOD mice with interleukin 1 beta and interferon gamma, which induces surface expression of FAS, followed by exposure to soluble FAS ligand (FASL), the proportion of apoptotic beta cells increased from 13% (in the absence of FASL) to 32% in wild-type NOD islets but from 12% to only 17% in islets from transgenic mice

immune system

increased susceptibility to autoimmune diabetes ( J:132052 )

• whereas treatment with a single injection of streptozotoxin protects wild-type NOD mice from spontaneous development of diabetes, transgenic NOD mice are not thus protected

endocrine/exocrine glands

decreased susceptibility to induced pancreatitis ( J:132052 )

• although NOD mice with this transgene spontaneously develop diabetes with the same incidence and kinetics as their nontransgenic littermates, they are almost completely protected from trasference of diabetes by injection of splenocytes from spontaneously diabetic NOD mice following full-body irradiation