Phenotypes associated with this allele

• Allele Symbol: Igf2^tm4.1Wrk
• Allele Name: targeted mutation 4.1, Wolf Reik
• Allele ID: MGI:5310741
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Igf2^tm4.1Wrk/Igf2^+	involves: 129P2/OlaHsd * BALB/c * C57BL/6 * SD7	MGI:5310743
ht2	Igf2^tm1Rob/Igf2^tm4.1Wrk	involves: 129P2/OlaHsd * 129S/SvEv * BALB/c * C57BL/6	MGI:5310742

Genotype
MGI:5310743

ht1

• Allelic Composition: Igf2^tm4.1Wrk/Igf2⁺
• Genetic Background: involves: 129P2/OlaHsd * BALB/c * C57BL/6 * SD7

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal imprinting ( J:178292 )

• when the repressor site-disrupted allele is maternally inherited, its expression in 12.5-17.5 days post coitus (dpc) embryos (detected by RT-PCR followed by restriction enzyme digestion of the cDNA to distinguish it from that from the paternally inherited M. spretus allele) is ~3-fold that of the normally repressed wild-type maternal allele in control embryos.

• when the disrupted allele is paternally inherited, its expression level does not appear to differ significantly from that of the highly expressed wild-type paternal allele of control embryos.

Genotype
MGI:5310742

ht2

• Allelic Composition: Igf2^tm1Rob/Igf2^tm4.1Wrk
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * BALB/c * C57BL/6

cellular

abnormal imprinting ( J:178292 )

• quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis of mRNA extracted from 8-10 pooled, whole 12.5-17.5 days post coitus (dpc) embryos with a nonfunctional paternal Igf2 allele demonstrates that those with a repressor binding site-disrupted maternal Igf2 allele express approximately 3-fold the amount of Igf2 mRNA as embryos with a normally repressed wild-type maternal allele; this is confirmed by RNAse protection analysis.

• while whole-embryo results primarily reflect expression in the mesoderm, analysis of placenta, yolk sac and endoderm-derived tissues including liver produces similar results.

growth/size/body

growth/size/body region phenotype ( J:169366 )

N • unlike pups that inherit an Igf2 allele whose repressor binding site is disrupted by the floxed neo^r cassette, pups with the present, loxP-only disruption do not differ in size from their littermates.