All Phenotypes Ift140<tm1c(KOMP)Wtsi> MGI Mouse

reproductive system phenotype ( J:285527 )

N	• males show normal testis size with no significant differences in testis weight and testis/body weight ratio relative to control males

oligozoospermia ( J:285527 )

• sperm count is significantly reduced; only few sperm are found in the lumen of cauda epididymis

teratozoospermia ( J:285527 )

• SEM analysis revealed epididymal sperm with amorphous heads, short/swollen flagella, uneven thickness of flagella, and other distorted shapes

abnormal sperm flagellum morphology ( J:285527 )

• cauda epididymal sperm from 3- to 4-mo-old males display short and/or bent tails

• vacuoles are present at the base of stunted flagella

abnormal outer dense fiber morphology ( J:285527 )

• misplaced ODFs are observed

abnormal sperm axoneme morphology ( J:285527 )

• some sperm lack a 9 + 2 core axoneme structure

• TEM analysis revealed that the few epididymal and testicular sperm recovered show disorganized axoneme microtubule array

abnormal sperm end piece morphology ( J:285527 )

• some sperm display a swollen tail tip

abnormal sperm mitochondrial morphology ( J:285527 )

• misplaced mitochondria components are observed

abnormal sperm fibrous sheath morphology ( J:285527 )

• misplaced fibrous sheath components are observed

kinked sperm flagellum ( J:285527 )

short sperm flagellum ( J:285527 )

abnormal sperm head morphology ( J:285527 )

• amorphous sperm heads are observed

abnormal sperm nucleus morphology ( J:285527 )

• some sperm show disorganized chromatin structure

abnormal spermatid morphology ( J:285527 )

• in round spermatids, IFT20 protein does not appear to attach as tightly to the nuclear membrane as in control spermatids

• IFT27 protein appears to form a large vesicle, not observed in control round spermatids

abnormal manchette morphology ( J:285527 )

• although IFT88 protein is still present in the cytoplasm of spermatocytes and round spermatids, it is absent from the manchette of elongating spermatids and shows abnormal accumulation in the cells at later stages

decreased sperm progressive motility ( J:285527 )

• a small % of sperm exhibit low beating amplitude with limited forward movement; curvilinear velocity is largely reduced

asthenozoospermia ( J:285527 )

• less than 10% of sperm remain motile; however, their motility is significantly weaker than that of control sperm

immotile sperm ( J:285527 )

• >90% of sperm display no mobility

abnormal spermiogenesis ( J:285527 )

• step 16 spermatids are phagocytosed along the basement membrane after stage VIII

• abnormal shapes of the developing spermatid heads appear in stage X

• an excessive accumulation of cytoplasm is observed in elongating spermatids beginning in stage XII

• epididymides contain round bodies of cytoplasm derived from the sloughing of the cytoplasmic lobes and residual bodies

abnormal spermiation ( J:285527 )

• failure of spermiation in stage VIII leading to a reduction of sperm numbers in the cauda epididymis

• only few sperm are detected in the lumen of seminiferous tubules

male infertility ( J:285527 )

• when mated with adult wild-type females for at least 2 months, all 6-wk-old or adult males tested fail to produce offspring, indicating complete male sterility

• however, males show normal sexual behavior and vaginal plug formation

impaired fertilization ( J:285527 )

• in an in vitro fertilization assay, only 5 of 84 C57BL/6J oocytes incubated with mutant sperm progressed to the 2-cell stage; three of these appeared markedly abnormal and none progressed to the 4-cell stage