Phenotypes associated with this allele

• Allele Symbol: Tg(CAG-Tfb1m)AGsha
• Allele Name: transgene insertion A, Gerald Shadel
• Allele ID: MGI:5313807
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Prkaa2^tm1Vio/Prkaa2^+ Tg(CAG-Tfb1m)AGsha/0	involves: 129S2/SvPas * C57BL/6J * SJL/J	MGI:5789745
cx2	E2f1^tm1Meg/E2f1^+ Tg(CAG-Tfb1m)AGsha/0	involves: 129S4/SvJae * C57BL/6J * SJL/J	MGI:5488619
tg3	Tg(CAG-Tfb1m)AGsha/0	B6J.Cg-Tg(CAG-Tfb1m)AGsha	MGI:5789744
tg4	Tg(CAG-Tfb1m)AGsha/?	involves: C57BL/6J * SJL/J	MGI:5488618

Genotype
MGI:5789745

cx1

• Allelic Composition: Prkaa2^tm1Vio/Prkaa2⁺; Tg(CAG-Tfb1m)AGsha/0
• Genetic Background: involves: 129S2/SvPas * C57BL/6J * SJL/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hearing/vestibular/ear

hearing/vestibular/ear phenotype ( J:228068 )

N • ABR thresholds are similar to wild-type controls, indicating rescue or delay of the premature hearing loss seen in single Tg(CAG-Tfb1m)AGsha transgenic mice

Genotype
MGI:5488619

cx2

• Allelic Composition: E2f1^tm1Meg/E2f1⁺; Tg(CAG-Tfb1m)AGsha/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6J * SJL/J

Genotype
MGI:5789744

tg3

• Allelic Composition: Tg(CAG-Tfb1m)AGsha/0
• Genetic Background: B6J.Cg-Tg(CAG-Tfb1m)AGsha

hearing/vestibular/ear

decreased endocochlear potential ( J:228068 )

• 40% reduction in endocochlear potential in 9 to 12 month old mice

abnormal cochlear outer hair cell electromotility ( J:228068 )

• outer hair cells exhibit a positive shift of nearly 20 mV in the voltage at peak capacitance, indicating a mild impairment of voltage-dependent electromotility

• however, hair cells in the apex of the cochlea are intact

abnormal auditory brainstem response waveform shape ( J:228068 )

• mice exhibit increased latency to peak I at 8 kHz of the ABR at 9-12 months of age but not at 3-6 months of age, indicating dysfunctional cochlear and/or nerve VIII processing only in older mice with hearing loss

• ABR peak I amplitudes are reduced in 9-12 month old mice

• however, central conduction time reflecting synaptic and nerve conduction timing after cochlear/nerve VIII processing is unchanged in mice with severe hearing loss

increased or absent threshold for auditory brainstem response ( J:228068 )

• ABR thresholds are prematurely elevated

impaired hearing ( J:228068 )

• progressive hearing loss

nervous system

abnormal cochlear outer hair cell electromotility ( J:228068 )

• outer hair cells exhibit a positive shift of nearly 20 mV in the voltage at peak capacitance, indicating a mild impairment of voltage-dependent electromotility

• however, hair cells in the apex of the cochlea are intact

Genotype
MGI:5488618

tg4

• Allelic Composition: Tg(CAG-Tfb1m)AGsha/?
• Genetic Background: involves: C57BL/6J * SJL/J