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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Aicdatm1(cre)Njen
targeted mutation 1, Nancy A Jenkins
MGI:5314932
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Dicer1tm1Bdh/Dicer1tm1Bdh
Aicdatm1(cre)Njen/Aicda+ 		involves: 129 * C57BL/6 MGI:5315120
cn2
 		Bcl2l11tm1Ast/Bcl2l11tm1Ast
Dicer1tm1Bdh/Dicer1tm1Bdh
Aicdatm1(cre)Njen/Aicda+ 		involves: 129S1/Sv * C57BL/6 MGI:5315121


Genotype
MGI:5315120
cn1
Allelic
Composition		 Dicer1tm1Bdh/Dicer1tm1Bdh
Aicdatm1(cre)Njen/Aicda+
Genetic
Background		 involves: 129 * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Aicdatm1(cre)Njen mutation (0 available); any Aicda mutation (57 available)
Dicer1tm1Bdh mutation (4 available); any Dicer1 mutation (96 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:181782 )
N
• before immunization, mice have normal B-cell subsets in the bone marrow, spleen, lymph nodes, and peritoneal cavities
decreased germinal center B cell number ( J:181782 )
• significantly decreased numbers (6- to 10 fold) of germinal center (GC) B cells are present in the spleen at day 10 after immunization
• drastic reductions in GC B cell populations in Peyer patches and mesenteric lymph nodes are also observed
absent memory B cells ( J:181782 )
• NP-specific memory B (IgG1) cells are essentially absent from spleens of mice 56 days after primary immunization with NP-CGG
absent plasma cells ( J:181782 )
• NP-specific IgG1 antibody-secreting cells (ASCs) are absent from the bone marrow at 56 days after primary immunization with NP-CGG
abnormal germinal center B cell physiology ( J:181782 )
• at 10 days after immunization, numbers of proliferating GC B cells are 3- to 5-fold lower than in wild-type, suggesting impaired proliferative capacity
• GC B cells are mores susceptible to cell death; numbers of apoptotic cells are 30 to 50% higher than in controls at day 10 after immunization
abnormal humoral immune response ( J:181782 )
• upon immunization with a T cell dependent antigen (NP-CGG), mice display normal levels of NP-specific IgM antibodies at 14, 21, and 28 days following exposure, while NP-specific IgG1, IgGb and IgG3 antibody titers are drastically reduced
decreased immunoglobulin level ( J:181782 )
• mice have lower basal serum levels of IgG1, IgG2b, IgG3, and IgA compared to wild-type
• IgM level is normal

hematopoietic system
decreased germinal center B cell number ( J:181782 )
• significantly decreased numbers (6- to 10 fold) of germinal center (GC) B cells are present in the spleen at day 10 after immunization
• drastic reductions in GC B cell populations in Peyer patches and mesenteric lymph nodes are also observed
absent memory B cells ( J:181782 )
• NP-specific memory B (IgG1) cells are essentially absent from spleens of mice 56 days after primary immunization with NP-CGG
absent plasma cells ( J:181782 )
• NP-specific IgG1 antibody-secreting cells (ASCs) are absent from the bone marrow at 56 days after primary immunization with NP-CGG
abnormal germinal center B cell physiology ( J:181782 )
• at 10 days after immunization, numbers of proliferating GC B cells are 3- to 5-fold lower than in wild-type, suggesting impaired proliferative capacity
• GC B cells are mores susceptible to cell death; numbers of apoptotic cells are 30 to 50% higher than in controls at day 10 after immunization
decreased immunoglobulin level ( J:181782 )
• mice have lower basal serum levels of IgG1, IgG2b, IgG3, and IgA compared to wild-type
• IgM level is normal




Genotype
MGI:5315121
cn2
Allelic
Composition		 Bcl2l11tm1Ast/Bcl2l11tm1Ast
Dicer1tm1Bdh/Dicer1tm1Bdh
Aicdatm1(cre)Njen/Aicda+
Genetic
Background		 involves: 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Aicdatm1(cre)Njen mutation (0 available); any Aicda mutation (57 available)
Bcl2l11tm1Ast mutation (0 available); any Bcl2l11 mutation (36 available)
Dicer1tm1Bdh mutation (4 available); any Dicer1 mutation (96 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:181782 )
• rescued germinal center B cells cannot effectively differentiate into antibody-secreting cells (AScs), and fail to differentiate into high affinity ASCs
decreased germinal center B cell number ( J:181782 )
• numbers are reduced in spleen relative to wild-type, but are dramatically increased on a Bcl2l11-deficient background compared to conditional knockouts with wild-type background
decreased plasma cell number ( J:181782 )
• NP-specific IgG1 antibody-secreting cells (ASCs) are present only at 20-25% of the numbers observed in wild-type spleens at day 12 after immunization
• high affinity ASCs are almost undetectable and bone marrow

hematopoietic system
abnormal B cell differentiation ( J:181782 )
• rescued germinal center B cells cannot effectively differentiate into antibody-secreting cells (AScs), and fail to differentiate into high affinity ASCs
decreased germinal center B cell number ( J:181782 )
• numbers are reduced in spleen relative to wild-type, but are dramatically increased on a Bcl2l11-deficient background compared to conditional knockouts with wild-type background
decreased plasma cell number ( J:181782 )
• NP-specific IgG1 antibody-secreting cells (ASCs) are present only at 20-25% of the numbers observed in wild-type spleens at day 12 after immunization
• high affinity ASCs are almost undetectable and bone marrow




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory