Analysis Tools|
Allele Symbol Allele Name Allele ID |
Mirc13tm1.1Mtm targeted mutation 1.1, Michael McManus MGI:5315598 |
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| Summary |
2 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice fed a methionine- and choline-deficient (MCD) diet for 2 weeks (to induce nonalcoholic steatohepatitis) show markedly lower liver TG contents than MCD-fed wild-type controls
• lipidomics analysis showed that, upon MCD feeding, most TG species are reduced in the liver
• however, fasting serum TG levels are not different on the control diet (CD) or MCD diet
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• MCD-fed mice show significantly lower absolute and relative liver weights than MCD-fed wild-type controls
• however, no differences are noted between groups on the CD diet
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• MCD-fed mice show significantly lower absolute and relative liver weights, liver triglyceride contents, hepatic accumulation of lipid droplets, and infiltration of inflammatory cells than MCD-fed wild-type controls; moreover, serum AST and ALT levels are not significantly elevated, serum IL-6 levels are reduced, F4/80-positive Kupffer cell are less abundant, and hepatic acute phase response genes (Saa1 and Orm1), inflammatory cytokines (Il1b, Tnf, Il4), macrophage and neutrophil markers (F4/80 and LyG6), and fibrotic genes (Col1a1, Tgfb1) are induced to a lesser extent than in MCD-fed controls
• however, MCD-fed mice show normal liver cholesterol and H2O2 levels relative to MCD-fed wild-type controls
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• mice fed a CD diet show significantly lower fasting serum glucose levels than CD-fed wild-type controls
• however, no differences are noted between groups on the MCD diet
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• MCD-fed mice show increased levels of MTTP (microsomal triglyceride transfer protein) protein in the liver, suggesting increased lipid transport
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• MCD-fed mice show a significant increase in liver cholic acid level
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• MCD-fed mice show a significant reduction in several saturated fatty acids (e.g., myristic acid and heptadecanoic acid)
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• MCD-fed mice show a significant increase in a large number of phospholipids in the liver, including phosphatidylinositol (PI), phosphatidylserine (PS), most phosphatidylcholine (PC) and many of the phosphatidylethanolamine (PE) species
• however, liver fatty acid levels are not significantly altered
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• MCD-fed mice show a significant increase in most sphingomyelin (SM) species in the liver
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• MCD-fed mice show a significant increase in ceramide lipid species (esp. d41:1, d43:1, and d44:1)
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• mice fed a methionine- and choline-deficient (MCD) diet for 2 weeks (to induce nonalcoholic steatohepatitis) show markedly lower liver TG contents than MCD-fed wild-type controls
• lipidomics analysis showed that, upon MCD feeding, most TG species are reduced in the liver
• however, fasting serum TG levels are not different on the control diet (CD) or MCD diet
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• MCD-fed mice show significantly altered metabolites in the liver, including increased isohexonic acid, cholic acid, glycerol, glyceric acid, myoinositol, heptadecanoic acid, myristic acid, aminomalonate, lauric acid, histidine, and ornithine
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• MCD-fed mice show a modest decrease in thiobarbituric acid reactive substances (TBARs) in liver homogenates
• however, serum TBARs (a measure of lipid peroxidation) are similar to those in MCD-fed wild-type controls
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• MCD-fed mice show markedly increased protein levels of pSREPB1 (the precursor form of SREBP1, a master regulator of lipogenesis) but decreased levels of its nuclear form (nSREPB1) in the liver; in addition, hepatic protein levels of FASN (fatty acid synthase) are downregulated, suggesting decreased de novo lipid synthesis
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• MCD-fed mice show reduced mRNA levels of iNOS (an M1 polarization marker) with a concomitant increase in mRNA levels of Arg1 (an M2 polarization marker), suggesting an increase in alternatively activated anti-inflammatory macrophages
• in vitro, LPS-treated BM-derived macrophages polarize the macrophage phenotype toward a M2-like anti-inflammatory state, as shown by induction of Arg1 and IL-10 mRNA expression; in contrast, induction of Il6 and Il1b mRNA by LPS is reduced, suggesting that a proinflammatory M1-like state is less favored
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• MCD-fed mice show reduced mRNA levels of iNOS (an M1 polarization marker) with a concomitant increase in mRNA levels of Arg1 (an M2 polarization marker), suggesting an increase in alternatively activated anti-inflammatory macrophages
• in vitro, LPS-treated BM-derived macrophages polarize the macrophage phenotype toward a M2-like anti-inflammatory state, as shown by induction of Arg1 and IL-10 mRNA expression; in contrast, induction of Il6 and Il1b mRNA by LPS is reduced, suggesting that a proinflammatory M1-like state is less favored
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N |
• mice exhibit normal body weights both before and after a 2-week MCD feeding regimen
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 10/29/2024 MGI 6.24 |
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