Phenotypes associated with this allele

• Allele Symbol: Slc12a5^tm2.1Tjj
• Allele Name: targeted mutation 2.1, Thomas J Jentsch
• Allele ID: MGI:5315721
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Slc12a5^tm2.1Tjj/Slc12a5^tm2.1Tjj Gabra6^tm2(cre)Wwis/Gabra6^+	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5315753
cn2	Slc12a5^tm2.1Tjj/Slc12a5^tm2.1Tjj Gabra6^tm2(cre)Wwis/Gabra6^+ Tg(Pcp2-cre)2Mpin/0	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5315754
cn3	Slc12a5^tm2.1Tjj/Slc12a5^tm2.1Tjj Tg(Pcp2-cre)2Mpin/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5315752

Genotype
MGI:5315753

cn1

• Allelic Composition: Slc12a5^tm2.1Tjj/Slc12a5^tm2.1Tjj; Gabra6^tm2(cre)Wwis/Gabra6⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

nervous system phenotype ( J:181930 )

N • mice exhibit normal cerebellar histology, synaptic morphology and synaptic function

abnormal cerebellar granule cell morphology ( J:181930 )

• resting membrane potential mediated by chloride ion extrusion in granule cells is disrupted compared to in wild-type mice

abnormal neuron morphology ( J:181930 )

• chloride ion concentration is increased 2-fold compared to in wild-type mice

abnormal action potential ( J:181930 )

• spontaneous Purkinje cell firing is irregular compared to in wild-type cells

behavior/neurological

behavior/neurological phenotype ( J:181930 )

N • mice exhibit normal short-term, gain-decreased motor learning and dark, gain consolidation

abnormal learning/memory/conditioning ( J:181930 )

• mice exhibit impaired vestibuloocular reflex in the dark phase adjustment from day 4 (long-term memory consolidation) compared with wild-type mice

Genotype
MGI:5315754

cn2

• Allelic Composition: Slc12a5^tm2.1Tjj/Slc12a5^tm2.1Tjj; Gabra6^tm2(cre)Wwis/Gabra6⁺; Tg(Pcp2-cre)2Mpin/0
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6

behavior/neurological

abnormal learning/memory/conditioning ( J:181930 )

• mice exhibit impairment in vestibuloocular reflex in the dark decrease learning and dark, gain consolidation compared with wild-type mice

• mice exhibit impaired vestibuloocular reflex in the dark phase adjustment from day 4 (long-term memory consolidation) compared with wild-type mice

abnormal motor learning ( J:181930 )

• mice exhibit impairment in vestibuloocular reflex in the dark decrease learning and dark, gain consolidation compared with wild-type mice

abnormal optokinetic reflex ( J:181930 )

• mice exhibit decreased gain in optokinetic reflex compared with wild-type mice

• mice exhibit a phase lag during optokinetic reflex compared with wild-type mice

abnormal vestibuloocular dark reflex ( J:181930 )

• mice exhibit a phase lag during vestibuloocular reflex in the dark compared with wild-type mice

• mice exhibit impairment in vestibuloocular reflex in the dark decrease learning compared with wild-type mice

abnormal vestibuloocular light reflex ( J:181930 )

• mice exhibit a phase lag during vestibuloocular reflex in the light compared with wild-type mice

nervous system

nervous system phenotype ( J:181930 )

N • mice exhibit normal cerebellar histology, synaptic morphology and synaptic function

abnormal GABA-mediated receptor currents ( J:181930 )

• mice exhibit reduced GABA-induced chloride ion currents compared with wild-type mice that is not as severe as in Slc12a5^tm2.1Tjj/Slc12a5^tm2.1Tjj Tg(Pcp2-cre)2Mpin mice

hearing/vestibular/ear

abnormal vestibuloocular dark reflex ( J:181930 )

• mice exhibit a phase lag during vestibuloocular reflex in the dark compared with wild-type mice

• mice exhibit impairment in vestibuloocular reflex in the dark decrease learning compared with wild-type mice

abnormal vestibuloocular light reflex ( J:181930 )

• mice exhibit a phase lag during vestibuloocular reflex in the light compared with wild-type mice

Genotype
MGI:5315752

cn3

• Allelic Composition: Slc12a5^tm2.1Tjj/Slc12a5^tm2.1Tjj; Tg(Pcp2-cre)2Mpin/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

nervous system

nervous system phenotype ( J:181930 )

N • mice exhibit normal cerebellar histology, synaptic morphology and synaptic function

abnormal neuron morphology ( J:181930 )

• chloride ion concentration is increased 2-fold compared to in wild-type mice

abnormal Purkinje cell morphology ( J:181930 )

• GABA-induced hyperpolarization in Purkinje cells is nearly abolished compared to in wild-type cells

• regularity of Purkinje cell firing is reduced compared to in wild-type cells

impaired ability to fire action potentials ( J:181930 )

• regularity of Purkinje cell firing is reduced compared to in wild-type cells

abnormal GABA-mediated receptor currents ( J:181930 )

• GABA-induced hyperpolarization in Purkinje cells is nearly abolished compared to in wild-type cells

behavior/neurological

abnormal learning/memory/conditioning ( J:181930 )

• mice exhibit impairment in vestibuloocular reflex in the dark decrease learning and dark, gain consolidation compared with wild-type mice

• in a long-term vestibuloocular reflex in the dark phase reversal training paradigm, mice are unable to reverse the phase of their eye movements unlike wild-type mice

abnormal motor learning ( J:181930 )

• mice exhibit impairment in vestibuloocular reflex in the dark decrease learning and dark, gain consolidation compared with wild-type mice

abnormal optokinetic reflex ( J:181930 )

• mice exhibit decreased gain in optokinetic reflex compared with wild-type mice

vision/eye

vision/eye phenotype ( J:181930 )

N • mice exhibit normal retina function in vestibuloocular reflex adaptation