Phenotypes associated with this allele

• Allele Symbol: Dido1^tm2Cmar
• Allele Name: targeted mutation 2, Carlos Martinez-A
• Allele ID: MGI:5318886
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dido1^tm2Cmar/Dido1^tm2Cmar	involves: 129S1/Sv * 129X1/SvJ	MGI:5318887
ht2	Dido1^tm1Cmar/Dido1^tm2Cmar	involves: 129S1/Sv * 129X1/SvJ	MGI:5318888

Genotype
MGI:5318887

hm1

• Allelic Composition: Dido1^tm2Cmar/Dido1^tm2Cmar
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality between somite formation and embryo turning, complete penetrance ( J:183346 )

• no embryos survive past E8.5

embryo

embryonic growth arrest ( J:183346 )

• in the epiblast at E7.5

abnormal embryonic epiblast morphology ( J:183346 )

• expression analysis indicates a defect in lineage commitment of epiblast cells at the onset of differentiation

decreased embryonic epiblast cell proliferation ( J:183346 )

• at E7.5

abnormal embryonic tissue physiology ( J:183346 )

• increased apoptosis and reduced proliferation primarily in the epiblast at E7.5

increased embryonic epiblast cell apoptosis ( J:183346 )

• at E7.5

cellular

abnormal centrosome morphology ( J:183346 )

• moderate but significant increase in cells with an abnormal centrosome number in cells from E7.5 embryos

• about 10% of cultured ES cells are aneuploid with multiple centrosomes

aneuploidy ( J:183346 )

• about 10% of cultured ES cells are aneuploid with multiple centrosomes

increased embryonic epiblast cell apoptosis ( J:183346 )

• at E7.5

abnormal cell differentiation ( J:183346 )

• impaired differentiation of cultured ES cells and reduced teratoma formation by ES cells when injected into immunodeficient mice

decreased embryonic epiblast cell proliferation ( J:183346 )

• at E7.5

Genotype
MGI:5318888

ht2

• Allelic Composition: Dido1^tm1Cmar/Dido1^tm2Cmar
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

mortality/aging

prenatal lethality, incomplete penetrance ( J:183346 )

• not born at the expected Mendelian frequency but survivors are viable

cellular

abnormal centrosome morphology ( J:183346 )

• centrosome amplification in MEFs

abnormal chromosome morphology ( J:183346 )

• structural abnormalities are seen in aneuploid MEFs

aneuploidy ( J:183346 )

• MEFs show about a 2 fold increase in aneuploid cells

abnormal mitosis ( J:183346 )

• premature sister chromatid exchange in MEFs