Phenotypes associated with this allele

• Allele Symbol: Hk2^tm1.1Uku
• Allele Name: targeted mutation 1.1, University of Kuopio
• Allele ID: MGI:5320615
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hk2^tm1.1Uku/Hk2^tm1.1Uku	B6.129P2-Hk2^tm1.1Uku/Kctt	MGI:5432256
cn2	Hk2^tm1.1Uku/Hk2^tm1.1Uku Tg(GFAP-cre)25Mes/0	B6.Cg-Hk2^tm1.1Uku Tg(GFAP-cre)25Mes	MGI:5571382
cn3	Gt(ROSA)26Sor^tm1(Smo/EYFP)Amc/Gt(ROSA)26Sor^+ Hk2^tm1.1Uku/Hk2^tm1.1Uku Tg(GFAP-cre)25Mes/0	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * FVB/N	MGI:5571383
cn4	Gt(ROSA)26Sor^tm1(Smo/EYFP)Amc/Gt(ROSA)26Sor^+ Hk2^tm1.1Uku/Hk2^+ Tg(GFAP-cre)25Mes/0	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * FVB/N	MGI:5571384

Genotype
MGI:5432256

hm1

• Allelic Composition: Hk2^tm1.1Uku/Hk2^tm1.1Uku
• Genetic Background: B6.129P2-Hk2^tm1.1Uku/Kctt

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:82809 )

• no defects observed without cre-recombinase activity

Genotype
MGI:5571382

cn2

• Allelic Composition: Hk2^tm1.1Uku/Hk2^tm1.1Uku; Tg(GFAP-cre)25Mes/0
• Genetic Background: B6.Cg-Hk2^tm1.1Uku Tg(GFAP-cre)25Mes

nervous system

abnormal cerebellar granule cell migration ( J:210113 )

• cerebellar granule neuron progenitors fail to migrate over the Purkinje cell layer

abnormal neuronal precursor proliferation ( J:210113 )

• at P7, cerebellar granule neuron progenitors exhibit reduced proliferation in response to Shh compared with wild-type cells

abnormal cerebellum external granule cell layer morphology ( J:210113 )

• focal disorganization of the external granule cell layer

• regions of focal thinning or thickening, abnormal migration and increased vascularization at P7

irregular external granule cell layer thickness ( J:210113 )

• regions of focal thinning or thickening at P7

cellular

abnormal cerebellar granule cell migration ( J:210113 )

• cerebellar granule neuron progenitors fail to migrate over the Purkinje cell layer

abnormal neuronal precursor proliferation ( J:210113 )

• at P7, cerebellar granule neuron progenitors exhibit reduced proliferation in response to Shh compared with wild-type cells

Genotype
MGI:5571383

cn3

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Smo/EYFP)Amc}/Gt(ROSA)26Sor⁺; Hk2^tm1.1Uku/Hk2^tm1.1Uku; Tg(GFAP-cre)25Mes/0
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * FVB/N

mortality/aging

premature death ( J:210113 )

• median survival is 31 days compared with mice with wild-type Hk2 that die by P20

neoplasm

abnormal tumor vascularization ( J:210113 )

• increased micro-vascularization

decreased tumor growth/size ( J:210113 )

• compared to in mice with wild-type Hk2

increased medulloblastoma incidence ( J:210113 )

• all mice develop tumors with reduced growth and at increased latency compared to in mice with wild-type Hk2

increased tumor latency ( J:210113 )

• all mice develop tumors with increased latency compared to in mice with wild-type Hk2

nervous system

abnormal neuronal precursor proliferation ( J:210113 )

• reduced cerebellar granule neuron progenitors proliferation

increased medulloblastoma incidence ( J:210113 )

• all mice develop tumors with reduced growth and at increased latency compared to in mice with wild-type Hk2

cellular

abnormal neuronal precursor proliferation ( J:210113 )

• reduced cerebellar granule neuron progenitors proliferation

cardiovascular system

abnormal tumor vascularization ( J:210113 )

• increased micro-vascularization

Genotype
MGI:5571384

cn4

• Allelic Composition: Gt(ROSA)26Sor^{tm1(Smo/EYFP)Amc}/Gt(ROSA)26Sor⁺; Hk2^tm1.1Uku/Hk2⁺; Tg(GFAP-cre)25Mes/0
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * FVB/N

mortality/aging

premature death ( J:210113 )

• mice die by P20

neoplasm

increased medulloblastoma incidence ( J:210113 )

nervous system

increased medulloblastoma incidence ( J:210113 )