mortality/aging
• embryonic lethality by E9
|
embryo
N |
• gross morphology is normal at E5.5
|
• at E7.5
|
• visceral endoderm lacks apical vacuoles and accumulates electron dense spherical structures in the cytoplasm at E6.5
• accumulate eosinophilic granules at E5.5
• distally located cells remain columnar or cuboidal
|
• impaired mesoderm growth at E8.5
|
• embryonic region is smaller than in controls
|
• embryos remain as deformed egg cylinders at E8.5
|
• little if any lateral plate mesoderm
|
cellular
• at E7.5
|
• defects in late stage endocytic pathway at E6.2
|