Phenotypes associated with this allele

• Allele Symbol: Foxa2^{tm3.1(icre)Heli}
• Allele Name: targeted mutation 3.1, Heiko Lickert
• Allele ID: MGI:5426440
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Hes1^tm1Kag/Hes1^tm1Kag Foxa2^tm3.1(icre)Heli/Foxa2^+	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:5428766
cn2	Mib1^tm2Kong/Mib1^tm2Kong Foxa2^tm3.1(icre)Heli/Foxa2^+	involves: 129P2/OlaHsd * C57BL/6 * SJL	MGI:5428765
cn3	Gt(ROSA)26Sor^tm1.1(Maml1/EGFP)Hri/? Foxa2^tm3.1(icre)Heli/Foxa2^+	involves: 129P2/OlaHsd * C57BL/6 * SJL	MGI:5428767
cn4	Dll1^tm1.1Hri/Dll1^tm1.1Hri Foxa2^tm3.1(icre)Heli/Foxa2^+	involves: 129P2/OlaHsd * C57BL/6 * SJL	MGI:5428768

Genotype
MGI:5428766

cn1

• Allelic Composition: Hes1^tm1Kag/Hes1^tm1Kag; Foxa2^{tm3.1(icre)Heli}/Foxa2⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

abnormal pancreas development ( J:184814 )

• embryos show an increase in Neurog3+ve cells at E9.5; by E10.5, an increase in glucagon+ve cells is seen

• at E15.5, the insulin +ve area of the pancreatic tissue is reduced by 65% compared to control embryos similar to the dnMaml-expressing embryos

• some DBA+ve cells are observed in E18.5 embryos

• proximal-distal patterning is altered in the embryonic pancreas; at E15.5, Nkx6-1+ve, Ptf1a-ve cells are reduced in the proximal domain by 85% while Hnf1b and DBA+ve cells are reduced in number with a concurrent increase in Nkx6-1-ve, Ptf1a+ve cells as well as Cpa+ve and amylase +ve cells

• Nkx6-1 and Ptf1a double-positive cells are observed in significant numbers

• at E12.5, Nkx6-1+ve Ptf1a-ve are present, but reduced in numbers by 50% compared to controls along with no increase in Nkx6-1-ve Ptf1a+ve cells

abnormal pancreatic beta cell differentiation ( J:184814 )

• at E15.5, the insulin +ve area of the pancreatic tissue is reduced by 65% compared to control embryos

cellular

abnormal pancreatic beta cell differentiation ( J:184814 )

• at E15.5, the insulin +ve area of the pancreatic tissue is reduced by 65% compared to control embryos

Genotype
MGI:5428765

cn2

• Allelic Composition: Mib1^tm2Kong/Mib1^tm2Kong; Foxa2^{tm3.1(icre)Heli}/Foxa2⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * SJL

endocrine/exocrine glands

absent pancreatic duct ( J:184814 )

• loss of DBA+ve (D. biflorus agglutinin) duct structures is observed by E18.5

abnormal pancreas development ( J:184814 )

• at E9.5, an excess of Neurog3+ve cells is found in the dorsal pancreatic bud; near complete conversion of dorsal bud cells to glucagon+ve cells occurs by E10.5

• at E10.5, dorsal pancreas is absent, but ventral pancreatic anlage forms normally

• proximal-distal patterning is altered in the embryonic pancreas; at E15.5, Nkx6-1+ve, Ptf1a-ve cells are absent from the proximal domain while Hnf1b and DBA+ve cells are also lost, with a concurrent increase in Nkx6-1-ve, Ptf1a+ve cells as well as Cpa+ve and amylase +ve cells

• at E12.5, Nkx6-1+ve Ptf1a-ve are present, but reduced in numbers by 50% compared to controls along with a corresponding increase in Nkx6-1-ve Ptf1a+ve cells

abnormal pancreatic beta cell differentiation ( J:184814 )

• insulin-producing cells are absent by E18.5

digestive/alimentary system

absent pancreatic duct ( J:184814 )

• loss of DBA+ve (D. biflorus agglutinin) duct structures is observed by E18.5

cellular

abnormal pancreatic beta cell differentiation ( J:184814 )

• insulin-producing cells are absent by E18.5

Genotype
MGI:5428767

cn3

• Allelic Composition: Gt(ROSA)26Sor^{tm1.1(Maml1/EGFP)Hri}/?; Foxa2^{tm3.1(icre)Heli}/Foxa2⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * SJL

endocrine/exocrine glands

abnormal pancreas development ( J:184814 )

• embryos show an increase in Neurog3+ve cells at E9.5; by E10.5, an increase in glucagon+ve cells is seen

• at E15.5, the insulin +ve area of the pancreatic tissue is reduced by 65% compared to control embryos

• proximal-distal patterning is altered in the embryonic pancreas; at E15.5, Nkx6-1+ve, Ptf1a-ve cells are reduced in the proximal domain by 75% while Hnf1b and DBA+ve cells are reduced in number

• at E12.5, Nkx6-1+ve Ptf1a-ve are present, but reduced in numbers by 50% compared to controls along with a corresponding increase in Nkx6-1-ve Ptf1a+ve cells

abnormal pancreatic beta cell differentiation ( J:184814 )

• at E15.5, the insulin +ve area of the pancreatic tissue is reduced by 65% compared to control embryos

cellular

abnormal pancreatic beta cell differentiation ( J:184814 )

• at E15.5, the insulin +ve area of the pancreatic tissue is reduced by 65% compared to control embryos

Genotype
MGI:5428768

cn4

• Allelic Composition: Dll1^tm1.1Hri/Dll1^tm1.1Hri; Foxa2^{tm3.1(icre)Heli}/Foxa2⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * SJL

endocrine/exocrine glands

abnormal pancreas development ( J:184814 )

• at E10.5, an excessive formation of glucagon +ve cells is observed

• reduced numbers of Nkx6-1+ve Ptf1a-ve cells are detected at E12.5

• however, no apparent defects in proximal-distal patterning in E15.5 and E18.5 embryos are observed