Phenotypes associated with this allele

• Allele Symbol: Wls^tm1Xzg
• Allele Name: targeted mutation 1, Xizhi Guo
• Allele ID: MGI:5426917
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Wls^tm1Xzg/Wls^tm1Xzg Tg(Prrx1-cre)1Cjt/0	involves: C57BL/6J * SJL/J	MGI:5426938
cn2	Wls^tm1Xzg/Wls^tm1Xzg Tg(Msx2-cre)5Rem/0	Not Specified	MGI:5426937

Genotype
MGI:5426938

cn1

• Allelic Composition: Wls^tm1Xzg/Wls^tm1Xzg; Tg(Prrx1-cre)1Cjt/0
• Genetic Background: involves: C57BL/6J * SJL/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality at weaning, incomplete penetrance ( J:184918 )

• most mice die at weaning due to failure to intake food and water caused by limb defects

limbs/digits/tail

decreased autopod size ( J:184918 )

• truncated

abnormal limb development ( J:184918 )

• at E12.5, mesenchymal cells exhibit decreased proliferation compared with wild-type cells

• at E13.5, distal limbs exhibit increased apoptosis compared with wild-type cells

• mice exhibit dysgenesis of limb soft tissue compared with wild-type mice

abnormal digit development ( J:184918 )

• at E12.5, digit rays fail to extend distally and a continuous ring of distal mesenchyme is retained

short limbs ( J:184918 )

• more severe in forelimbs than hindlimbs

cellular

increased apoptosis ( J:184918 )

• at E13.5, distal limbs exhibit increased apoptosis compared with wild-type cells

decreased cell proliferation ( J:184918 )

• of limb mesenchyme cells at E12.5

behavior/neurological

adipsia ( J:184918 )

• failure to intake food and water caused by limb defects

skeleton

abnormal skeleton morphology ( J:184918 )

• shortened

chondrodystrophy ( J:184918 )

• cartilage hypertrophy delayed

delayed bone ossification ( J:184918 )

Genotype
MGI:5426937

cn2

• Allelic Composition: Wls^tm1Xzg/Wls^tm1Xzg; Tg(Msx2-cre)5Rem/0
• Genetic Background: Not Specified

limbs/digits/tail

decreased autopod size ( J:184918 )

• truncated

oligodactyly ( J:184918 )

abnormal forelimb morphology ( J:184918 )

• dorsally flexed

abnormal forelimb stylopod morphology ( J:184918 )

• contains reduced and malformed muscle mass at E17.5

abnormal hindlimb morphology ( J:184918 )

• dorsally flexed

abnormal hindlimb zeugopod morphology ( J:184918 )

• shortened

short fibula ( J:184918 )

short tibia ( J:184918 )

abnormal limb development ( J:184918 )

• impaired patterning; more severe in the ventral limb than in dorsal limb

• at E10.5, mesenchymal cells exhibit decreased proliferation while apoptosis is increased in the distal limb mesenchyme compared with wild-type cells

• mice exhibit dysgenesis of limb soft tissue with progressive defects from proximal to distal compared with wild-type mice

abnormal apical ectodermal ridge morphology ( J:184918 )

• impaired development

thin apical ectodermal ridge ( J:184918 )

• thin and narrow at E10.5

short limbs ( J:184918 )

• more severe in hindlimbs than forelimbs

embryo

abnormal apical ectodermal ridge morphology ( J:184918 )

• impaired development

thin apical ectodermal ridge ( J:184918 )

• thin and narrow at E10.5

cellular

increased apoptosis ( J:184918 )

• of distal limb mesenchyme cells at E12.5

decreased cell proliferation ( J:184918 )

• of limb mesenchyme cells at E12.5

skeleton

short fibula ( J:184918 )

short tibia ( J:184918 )

abnormal tendon morphology ( J:184918 )

• in the forelimbs at E17.5

abnormal ligament morphology ( J:184918 )

• in the forelimbs at E17.5

delayed cranial suture closure ( J:184918 )

integument

abnormal hair follicle development ( J:184918 )

• limb hair follicle formation is blocked

abnormal dermal layer morphology ( J:184918 )

• limb dermis differentiation is blocked

muscle

abnormal muscle development ( J:184918 )

• in the forelimbs at E17.5

• impaired in the autopod

abnormal tendon morphology ( J:184918 )

• in the forelimbs at E17.5