Phenotypes associated with this allele

• Allele Symbol: Tg(Mpz-RAF1/ESR1)A668Ayld
• Allele Name: transgene insertion A668, Alison Lloyd
• Allele ID: MGI:5427464
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(Mpz-RAF1/ESR1)A668Ayld/0	involves: C57BL/6J * CBA/J	MGI:5487916

Genotype
MGI:5487916

tg1

• Allelic Composition: Tg(Mpz-RAF1/ESR1)A668Ayld/0
• Genetic Background: involves: C57BL/6J * CBA/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

impaired coordination ( J:182703 )

• mice treated with IP tamoxifen injections for five days exhibit impaired motor coordination as measured by accelerating rotarod test

impaired limb coordination ( J:182703 )

• mice treated with IP tamoxifen injections for five days exhibit severe impairment of coordination and positioning of their limbs by day 10

• in some cases, mice are unable to support their body weight

weakness ( J:182703 )

• mice treated with IP tamoxifen injections for five days exhibit weakness by days 7-8

nervous system

abnormal Schwann cell physiology ( J:182703 )

• following tamoxifen injections, myelinating Schwann cells are driven to a progenitor-like state (de-differentiation) resulting in loss of motor function

• decrease in Schwann cell/axon units containing compact myelin, remaining units exhibit myelin infoldings and outfoldings with vacuoles of degraded myelin

increased Schwann cell proliferation ( J:182703 )

• mice treated with tamoxifen exhibit an increase in cell proliferation in the nerves and a significant portion of these cells are Schwann cells

demyelination ( J:182703 )

• mice treated with tamoxifen exhibit loss of peripheral nerve function

• myelin breakdown is widespread and cellularity is increased in the intraneural spaces, however axons remain intact

• decrease in Schwann cell/axon units containing compact myelin, remaining units exhibit myelin infoldings and outfoldings with vacuoles of degraded myelin

• collagen-rich spaces increase in size between Schwann cell/axon units and contain cells not present in controls

immune system

increased mast cell number ( J:182703 )

• following tamoxifen injections

increased neutrophil cell number ( J:182703 )

• following tamoxifen injections

increased T cell number ( J:182703 )

• following tamoxifen injections

increased macrophage cell number ( J:182703 )

• following tamoxifen injections

increased inflammatory response ( J:182703 )

• large increase in macrophages, mast cells, neutrophils and CD3+ T cells following tamoxifen injections, but not fibroblasts

limbs/digits/tail

abnormal autopod morphology ( J:182703 )

• hindpaw prints are elongated and have reduced toespread as compared to controls following tamoxifen injections

cardiovascular system

abnormal blood vessel physiology ( J:182703 )

• breakdown of blood-nerve-barrier (BNB) occurs as early as day 4, with complete breakdown at by day 5 following tamoxifen injections

cellular

increased Schwann cell proliferation ( J:182703 )

• mice treated with tamoxifen exhibit an increase in cell proliferation in the nerves and a significant portion of these cells are Schwann cells

hematopoietic system

increased mast cell number ( J:182703 )

• following tamoxifen injections

increased neutrophil cell number ( J:182703 )

• following tamoxifen injections

increased T cell number ( J:182703 )

• following tamoxifen injections

increased macrophage cell number ( J:182703 )

• following tamoxifen injections