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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Pik3c3tm1.1Flv
targeted mutation 1.1, Richard A Flavell
MGI:5427838
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Pik3c3tm1.1Flv/Pik3c3tm1.1Flv
Tg(Cd8a-cre)1Itan/0 		involves: C57BL/6 * C57BL/6N MGI:5427890
cn2
 		Pik3c3tm1.1Flv/Pik3c3tm1.1Flv
Tg(Cd4-cre)1Cwi/0 		involves: C57BL/6 * C57BL/6N * DBA/2 MGI:5427889


Genotype
MGI:5427890
cn1
Allelic
Composition		 Pik3c3tm1.1Flv/Pik3c3tm1.1Flv
Tg(Cd8a-cre)1Itan/0
Genetic
Background		 involves: C57BL/6 * C57BL/6N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pik3c3tm1.1Flv mutation (0 available); any Pik3c3 mutation (48 available)
Tg(Cd8a-cre)1Itan mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:184753 )
N
• T cell development is normal
increased T cell apoptosis ( J:184753 )
• CD8+ T cells exhibit increased spontaneous apoptosis ex vivo compared with wild-type cells
decreased lymphocyte cell number ( J:184753 )
• T cell lymphopenia is cell autonomous based on bone marrow chimera experiments
abnormal CD4-positive, alpha beta T cell morphology ( J:184753 )
• CD4+ T cells exhibit increased mitochondrial mass compared with control cells
• CD4+ and CD8+ T cells exhibit increased reactive oxygen species compared with control cells
abnormal CD8-positive, alpha beta T cell morphology ( J:184753 )
• CD8+ T cells exhibit increased mitochondrial mass compared with control cells
• CD4+ and CD8+ T cells exhibit increased reactive oxygen species compared with control cells

cellular
abnormal mitochondrial morphology ( J:184753 )
• CD8+ and to a lesser extent CD4+ T cells exhibit increased mitochondrial mass compared with control cells
increased T cell apoptosis ( J:184753 )
• CD8+ T cells exhibit increased spontaneous apoptosis ex vivo compared with wild-type cells
abnormal mitochondrial physiology ( J:184753 )
• mitophagy is reduced in CD8+ and CD4+ T cells
oxidative stress ( J:184753 )
• CD4+ and CD8+ T cells exhibit increased reactive oxygen species compared with control cells

hematopoietic system
increased T cell apoptosis ( J:184753 )
• CD8+ T cells exhibit increased spontaneous apoptosis ex vivo compared with wild-type cells
decreased lymphocyte cell number ( J:184753 )
• T cell lymphopenia is cell autonomous based on bone marrow chimera experiments
abnormal CD4-positive, alpha beta T cell morphology ( J:184753 )
• CD4+ T cells exhibit increased mitochondrial mass compared with control cells
• CD4+ and CD8+ T cells exhibit increased reactive oxygen species compared with control cells
abnormal CD8-positive, alpha beta T cell morphology ( J:184753 )
• CD8+ T cells exhibit increased mitochondrial mass compared with control cells
• CD4+ and CD8+ T cells exhibit increased reactive oxygen species compared with control cells




Genotype
MGI:5427889
cn2
Allelic
Composition		 Pik3c3tm1.1Flv/Pik3c3tm1.1Flv
Tg(Cd4-cre)1Cwi/0
Genetic
Background		 involves: C57BL/6 * C57BL/6N * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pik3c3tm1.1Flv mutation (0 available); any Pik3c3 mutation (48 available)
Tg(Cd4-cre)1Cwi mutation (10 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal T cell number ( J:184753 )
• mainly due to loss of cells with naive (CD44lo) phenotype

cellular
abnormal autophagy ( J:184753 )
• T cells treated with BafA1 exhibit reduced autophagy activity compared with control cells

hematopoietic system
abnormal T cell number ( J:184753 )
• mainly due to loss of cells with naive (CD44lo) phenotype

homeostasis/metabolism
abnormal autophagy ( J:184753 )
• T cells treated with BafA1 exhibit reduced autophagy activity compared with control cells




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory