Phenotypes associated with this allele

• Allele Symbol: Prkch^tm1.2Gasc
• Allele Name: targeted mutation 1.2, Nicholas Gascoigne
• Allele ID: MGI:5431008
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prkch^tm1.2Gasc/Prkch^tm1.2Gasc	involves: C57BL/6 * FVB/N * SJL	MGI:5431087
cx2	Prkch^tm1.2Gasc/Prkch^tm1.2Gasc Prkcq^tm1Litt/Prkcq^tm1Litt	involves: 129P2/OlaHsd * C57BL/6 * FVB/N * SJL	MGI:5431088

Genotype
MGI:5431087

hm1

• Allelic Composition: Prkch^tm1.2Gasc/Prkch^tm1.2Gasc
• Genetic Background: involves: C57BL/6 * FVB/N * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased T cell proliferation ( J:186080 )

• when stimulated by anti-CD3 antibodies or antigens (in vitro and in vivo)

thymus hypoplasia ( J:186080 )

increased B cell number ( J:186080 )

• in the lymph node

increased T cell number ( J:186080 )

• in the lymph node

increased CD4-positive, alpha-beta T cell number ( J:186080 )

• in the lymph node

increased CD8-positive, alpha-beta T cell number ( J:186080 )

• in the lymph node

increased memory T cell number ( J:186080 )

decreased lymphocyte cell number ( J:186080 )

• in the spleen

decreased B cell number ( J:186080 )

• in the spleen

decreased T cell number ( J:186080 )

• in the spleen

decreased DN1 thymic pro-T cell number ( J:186080 )

decreased DN4 thymocyte number ( J:186080 )

decreased double-positive T cell number ( J:186080 )

• slightly

decreased CD4-positive, alpha-beta T cell number ( J:186080 )

• in the spleen

decreased CD8-positive, alpha-beta T cell number ( J:186080 )

• in the spleen

abnormal effector T cell morphology ( J:186080 )

• the ratio of CD4 to CD8 T cells is higher than in wild-type mice

abnormal lymph node morphology ( J:186080 )

• increased lymph node cell number

enlarged lymph nodes ( J:186080 )

• cervical, axillar, brachial and inguinal lymph nodes

enlarged axillary lymph nodes ( J:186080 )

abnormal brachial lymph node morphology ( J:186080 )

• enlarged

abnormal cervical lymph node morphology ( J:186080 )

• enlarged and increased in number

enlarged cervical lymph nodes ( J:186080 )

enlarged inguinal lymph nodes ( J:186080 )

hematopoietic system

decreased T cell proliferation ( J:186080 )

• when stimulated by anti-CD3 antibodies or antigens (in vitro and in vivo)

thymus hypoplasia ( J:186080 )

increased B cell number ( J:186080 )

• in the lymph node

increased T cell number ( J:186080 )

• in the lymph node

increased CD4-positive, alpha-beta T cell number ( J:186080 )

• in the lymph node

increased CD8-positive, alpha-beta T cell number ( J:186080 )

• in the lymph node

increased memory T cell number ( J:186080 )

decreased lymphocyte cell number ( J:186080 )

• in the spleen

decreased B cell number ( J:186080 )

• in the spleen

decreased T cell number ( J:186080 )

• in the spleen

decreased DN1 thymic pro-T cell number ( J:186080 )

decreased DN4 thymocyte number ( J:186080 )

decreased double-positive T cell number ( J:186080 )

• slightly

decreased CD4-positive, alpha-beta T cell number ( J:186080 )

• in the spleen

decreased CD8-positive, alpha-beta T cell number ( J:186080 )

• in the spleen

abnormal effector T cell morphology ( J:186080 )

• the ratio of CD4 to CD8 T cells is higher than in wild-type mice

endocrine/exocrine glands

thymus hypoplasia ( J:186080 )

decreased DN1 thymic pro-T cell number ( J:186080 )

decreased DN4 thymocyte number ( J:186080 )

cellular

decreased T cell proliferation ( J:186080 )

• when stimulated by anti-CD3 antibodies or antigens (in vitro and in vivo)

Genotype
MGI:5431088

cx2

• Allelic Composition: Prkch^tm1.2Gasc/Prkch^tm1.2Gasc; Prkcq^tm1Litt/Prkcq^tm1Litt
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * FVB/N * SJL

immune system

immune system phenotype ( J:186080 )

N • unlike in either single homozygote, the ratio of CD4 to CD8 T cells is normal

decreased T cell proliferation ( J:186080 )

• mildly impaired when stimulated by anti-CD3 antibodies

abnormal positive T cell selection ( J:186080 )

• strongly impaired

decreased double-negative T cell number ( J:186080 )

• greater than in single knock-out mice

decreased DN1 thymic pro-T cell number ( J:186080 )

• greater than in single knock-out mice

decreased DN4 thymocyte number ( J:186080 )

• greater than in single knock-out mice

decreased double-positive T cell number ( J:186080 )

• greater than in single knock-out mice

decreased CD4-positive, alpha-beta T cell number ( J:186080 )

• greater than in single knock-out mice

decreased CD8-positive, alpha-beta T cell number ( J:186080 )

• greater than in single knock-out mice

hematopoietic system

decreased T cell proliferation ( J:186080 )

• mildly impaired when stimulated by anti-CD3 antibodies

abnormal positive T cell selection ( J:186080 )

• strongly impaired

decreased double-negative T cell number ( J:186080 )

• greater than in single knock-out mice

decreased DN1 thymic pro-T cell number ( J:186080 )

• greater than in single knock-out mice

decreased DN4 thymocyte number ( J:186080 )

• greater than in single knock-out mice

decreased double-positive T cell number ( J:186080 )

• greater than in single knock-out mice

decreased CD4-positive, alpha-beta T cell number ( J:186080 )

• greater than in single knock-out mice

decreased CD8-positive, alpha-beta T cell number ( J:186080 )

• greater than in single knock-out mice

endocrine/exocrine glands

decreased DN1 thymic pro-T cell number ( J:186080 )

• greater than in single knock-out mice

decreased DN4 thymocyte number ( J:186080 )

• greater than in single knock-out mice

cellular

decreased T cell proliferation ( J:186080 )

• mildly impaired when stimulated by anti-CD3 antibodies