Phenotypes associated with this allele

• Allele Symbol: Grin2b^tm1Mony
• Allele Name: targeted mutation 1, Hannah Monyer
• Allele ID: MGI:5432023
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Grin2b^tm1Mony/Grin2b^tm1Mony Tg(Camk2a-Grin2c/itTA)12Rsp/0 Tg(tetO-cre)LC1Bjd/0	involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6	MGI:5432105
cn2	Grin2b^tm1Mony/Grin2b^tm1Mony Tg(Camk2a-cre)2Gsc/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N	MGI:5432103
cn3	Grin2b^tm1Mony/Grin2b^tm1Mony Tg(Camk2a-cre)1Gsc/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N	MGI:5432104

Genotype
MGI:5432105

cn1

• Allelic Composition: Grin2b^tm1Mony/Grin2b^tm1Mony; Tg(Camk2a-Grin2c/itTA)12Rsp/0; Tg(tetO-cre)LC1Bjd/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:186311 )

N • mice exhibit normal motor coordination and spatial reference memory acquisition in a Morris Water maze

abnormal spatial learning ( J:186311 )

• mice exhibit impaired spatial reversal learning compared with control mice

abnormal spatial working memory ( J:186311 )

• mice exhibit a small impairment in spatial working memory in an enclosed T maze compared with control mice

• less so than Grin2b^tm1Mony/Grin2b^tm1Mony Tg(Camk2a-cre)1Gsc mice

decreased anxiety-related response ( J:186311 )

• less so than Grin2b^tm1Mony/Grin2b^tm1Mony Tg(Camk2a-cre)1Gsc mice

hyperactivity ( J:186311 )

• less so than Grin2b^tm1Mony/Grin2b^tm1Mony Tg(Camk2a-cre)1Gsc mice

nervous system

abnormal excitatory postsynaptic currents ( J:186311 )

• in response to the Grin2b antagonist ifenprodil, mice fail to exhibit a strong reduction in NMDA excitatory postsynaptic currents and faster decay kinetics compared with control mice

abnormal NMDA-mediated synaptic currents ( J:186311 )

• in response to the Grin2b antagonist ifenprodil, mice fail to exhibit a strong reduction in NMDA excitatory postsynaptic currents and faster decay kinetics compared with control mice

Genotype
MGI:5432103

cn2

• Allelic Composition: Grin2b^tm1Mony/Grin2b^tm1Mony; Tg(Camk2a-cre)2Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N

mortality/aging

perinatal lethality, complete penetrance ( J:186311 )

Genotype
MGI:5432104

cn3

• Allelic Composition: Grin2b^tm1Mony/Grin2b^tm1Mony; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N

behavior/neurological

abnormal learning/memory/conditioning ( J:186311 )

• mice exhibit impaired egocentric spatial memory acquisition compared with control mice

abnormal discrimination learning ( J:186311 )

• impaired visual discrimination learning

abnormal object recognition memory ( J:186311 )

• impaired

abnormal spatial learning ( J:186311 )

• impaired in a Morris water maze

abnormal spatial reference memory ( J:186311 )

• impaired in a Morris water maze and appetitive, elevated Y maze

abnormal spatial working memory ( J:186311 )

• impaired in a discrete trial, spontaneous alternation paradigm in an enclosed T maze or a reward alternation on an elevated T maze

decreased anxiety-related response ( J:186311 )

• more so than in Grin2b^tm1Mony/Grin2b^tm1Mony Tg(Camk2a-Grin2c/itTA)12Rps Tg(tetO-cre)LC1Bjd mice

abnormal motor coordination/balance ( J:186311 )

• improved performance on a rotarod

hyperactivity ( J:186311 )

• more so than in Grin2b^tm1Mony/Grin2b^tm1Mony Tg(Camk2a-Grin2c/itTA)12Rps Tg(tetO-cre)LC1Bjd mice

nervous system

nervous system phenotype ( J:186311 )

N • mice exhibit normal cortical layer 4 whisker barrel structures and paired-pilse facilitation

abnormal excitatory postsynaptic currents ( J:186311 )

• ifenprodil-treated mice fail to exhibit an acceleration of NMDAR-mediated excitatory postsynaptic currents compared with wild-type mice

abnormal excitatory postsynaptic potential ( J:186311 )

• at high stimulation frequency, mice exhibit smaller maximal depolarization of the excitatory postsynaptic potentiation (EPSP) envelop and EPSP integral compared with control mice

• however, mice exhibit normal input resistance, action potential threshold, firing pattern and maximal firing frequency

abnormal NMDA-mediated synaptic currents ( J:186311 )

• mice fail to exhibit a reduction in NMDA currents in response to the Grin2b antagonist ifenprodil unlike control mice

• mice exhibit a faster deactivation kinetics (weighted tau) compared with control mice

• ifenprodil-treated mice fail to exhibit an acceleration of NMDAR-mediated excitatory postsynaptic currents compared with wild-type mice

reduced long-term potentiation ( J:186311 )

• low-frequency synaptic stimulation long term potentiation is reduced compared to in control mice

• during long term potentiation induction, charge transfer is reduced compared to in control mice

abnormal miniature excitatory postsynaptic currents ( J:186311 )

• miniature excitatory postsynaptic current amplitudes 50 ms are attenuated after onset unlike in control mice

growth/size/body

decreased body weight ( J:186311 )

• slightly