Phenotypes associated with this allele

• Allele Symbol: Sppl2a^m1Anu
• Allele Name: mutation 1, Australian National University
• Allele ID: MGI:5432126
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sppl2a^m1Anu/Sppl2a^m1Anu	involves: C57BL/6JAnu	MGI:5494310
cx2	Sppl2a^m1Anu/Sppl2a^m1Anu Tg(Vav-BCL2)69Jad/?	involves: C57BL/6JAnu	MGI:5494311

Genotype
MGI:5494310

hm1

• Allelic Composition: Sppl2a^m1Anu/Sppl2a^m1Anu
• Genetic Background: involves: C57BL/6JAnu

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal transitional stage B cell morphology ( J:194663 )

• 20 fold reduction of CD93^- IgM^low cells

decreased transitional stage T2 B cell number ( J:194663 )

• 20 fold reduction

decreased transitional stage T3 B cell number ( J:194663 )

• 20 fold reduction

abnormal mature B cell morphology ( J:194663 )

• number of mature IgD⁺ IgM^low recirculating B cells is selectively decreased and have very low levels of cell surface IgD

• 12% of normal levels of IgD and 30% of normal IgM on the cell surface

decreased marginal zone B cell number ( J:194663 )

• reduced 200 fold but localization to primary follicles is normal

abnormal immunoglobulin level ( J:194663 )

• almost no specific antibodies after immunization with various antigens

decreased IgG1 level ( J:194663 )

• serum levels decreased 6 fold

decreased IgM level ( J:194663 )

• serum levels decreased 25 fold

• cell surface levels are normal

hematopoietic system

hematopoietic system phenotype ( J:194663 )

N • normal bone marrow cellularity

N • normal pro-B, pre-B, and immature B cells

abnormal transitional stage B cell morphology ( J:194663 )

• 20 fold reduction of CD93^- IgM^low cells

decreased transitional stage T2 B cell number ( J:194663 )

• 20 fold reduction

decreased transitional stage T3 B cell number ( J:194663 )

• 20 fold reduction

abnormal mature B cell morphology ( J:194663 )

• number of mature IgD⁺ IgM^low recirculating B cells is selectively decreased and have very low levels of cell surface IgD

• 12% of normal levels of IgD and 30% of normal IgM on the cell surface

decreased marginal zone B cell number ( J:194663 )

• reduced 200 fold but localization to primary follicles is normal

abnormal immunoglobulin level ( J:194663 )

• almost no specific antibodies after immunization with various antigens

decreased IgG1 level ( J:194663 )

• serum levels decreased 6 fold

decreased IgM level ( J:194663 )

• serum levels decreased 25 fold

• cell surface levels are normal

Genotype
MGI:5494311

cx2

• Allelic Composition: Sppl2a^m1Anu/Sppl2a^m1Anu; Tg(Vav-BCL2)69Jad/?
• Genetic Background: involves: C57BL/6JAnu

immune system

increased plasmacytoid dendritic cell number ( J:194663 )

increased mature B cell number ( J:194663 )

• increase in splenic B cells over non mutant controls but lower numbers than in animals carrying the transgene alone

• CD93^- IgM^low mature B cells are increased 6.5X but CD93⁺ IgM^high T1 B cell number is normal

abnormal T cell number ( J:194663 )

decreased double-negative T cell number ( J:194663 )

• levels are about 12% of normal

decreased CD4-positive, alpha-beta T cell number ( J:194663 )

• levels are about 12% of normal

hematopoietic system

increased plasmacytoid dendritic cell number ( J:194663 )

increased mature B cell number ( J:194663 )

• increase in splenic B cells over non mutant controls but lower numbers than in animals carrying the transgene alone

• CD93^- IgM^low mature B cells are increased 6.5X but CD93⁺ IgM^high T1 B cell number is normal

abnormal T cell number ( J:194663 )

decreased double-negative T cell number ( J:194663 )

• levels are about 12% of normal

decreased CD4-positive, alpha-beta T cell number ( J:194663 )

• levels are about 12% of normal