Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj}
• Allele Name: targeted mutation 3, Alexandra L Joyner
• Allele ID: MGI:5432216
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gt(ROSA)26Sor^tm3(CAG-flpo/ERT2)Alj/Gt(ROSA)26Sor^tm3(CAG-flpo/ERT2)Alj	involves: 129S6/SvEvTac * C57BL/6	MGI:5432788
cn2	Gt(ROSA)26Sor^tm3(CAG-flpo/ERT2)Alj/Gt(ROSA)26Sor^+ Npm1^tm1Trow/Npm1^+	B6.Cg-Gt(ROSA)26Sor^tm3(CAG-flpo/ERT2)Alj Npm1^tm1Trow	MGI:6286134
cn3	Dnmt3a^tm1Trow/Dnmt3a^+ Gt(ROSA)26Sor^tm3(CAG-flpo/ERT2)Alj/Gt(ROSA)26Sor^+ Npm1^tm1Trow/Npm1^+ Tg(Mx1-cre)1Cgn/0	B6.Cg-Gt(ROSA)26Sor^tm3(CAG-flpo/ERT2)Alj Npm1^tm1Trow Dnmt3a^tm1Trow Tg(Mx1-cre)1Cgn	MGI:6286136
cn4	Smarce1^tm1Tich/Smarce1^tm2.1Tich Gt(ROSA)26Sor^tm3(CAG-flpo/ERT2)Alj/Gt(ROSA)26Sor^+ Tg(Cd4-cre)1Cwi/0	involves: 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:5474767

Genotype
MGI:5432788

hm1

• Allelic Composition: Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj}/Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj}
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:186310 )

• mice are viable and fertile with no reported phenotypic abnormalities

Genotype
MGI:6286134

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj}/Gt(ROSA)26Sor⁺; Npm1^tm1Trow/Npm1⁺
• Genetic Background: B6.Cg-Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj} Npm1^tm1Trow

hematopoietic system

abnormal bone marrow hematopoietic cell morphology ( J:272803 )

♀ • increase in total number of colony forming units are found in whole bone marrow cells 4 months post-tamoxifen injection

increased granulocyte monocyte progenitor cell number ( J:272803 )

♀ • expansion of granulocyte and granulocyte-macrophage colony types are found in whole bone marrow cells 4 months post-tamoxifen injection

decreased hematopoietic stem cell number ( J:272803 )

♀ • decrease in frequency and total number of long term hematopoietic stem cells 4 months post-tamoxifen injection

♀ • decrease in total number of short term hematopoietic stem cells 4 months post-tamoxifen injection

abnormal bone marrow cell physiology ( J:272803 )

♀ • bone marrow cells transplanted into lethally irradiated recipients and treated with pIpC and tamoxifen results in death in a 29% of animals in a 440 day period due to myeloproliferative disorder

Genotype
MGI:6286136

cn3

• Allelic Composition: Dnmt3a^tm1Trow/Dnmt3a⁺; Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj}/Gt(ROSA)26Sor⁺; Npm1^tm1Trow/Npm1⁺; Tg(Mx1-cre)1Cgn/0
• Genetic Background: B6.Cg-Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj} Npm1^tm1Trow Dnmt3a^tm1Trow Tg(Mx1-cre)1Cgn

hematopoietic system

abnormal bone marrow cell physiology ( J:272803 )

♀ • bone marrow cells transplanted into lethally irradiated recipients and treated with pIpC and tamoxifen results in death in a 100% of animals in a 440 day period

♀ • in 33% of mice death is the result of myeloproliferative disorder (MPD)

♀ • in 67% of mice death is the result of mixed myelodysplastic syndrome and myeloproliferative disorder (MDS/MPD)

♀ • bone marrow cells from MDS/MPD or MPD primary transplants transplanted into sublethally irradiated secondary recipients results in 100% lethality due to acute myeloid leukemia (AML)

Genotype
MGI:5474767

cn4

• Allelic Composition: Smarce1^tm1Tich/Smarce1^tm2.1Tich; Gt(ROSA)26Sor^{tm3(CAG-flpo/ERT2)Alj}/Gt(ROSA)26Sor⁺; Tg(Cd4-cre)1Cwi/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * DBA/2

immune system

immune system phenotype ( J:194195 )

N • tamoxifen-treated mice exhibit no gross functional defects in mature T cells