All Phenotypes Gja10<tm2(Hbegf/EGFP)Kwi> MGI Mouse

abnormal retina morphology ( J:186624 )

• the outer retina shows severe changes following horizontal cell ablation with diphtheria toxin (DT) treatment including loss of synaptic contacts and cone pedicle mosaic restructuring

abnormal retina cone cell morphology ( J:186624 )

• shape and arrangement of cone pedicles are changed following DT treatment; pedicles show atrophy and form complex clusters

abnormal retina rod cell morphology ( J:186624 )

• after DT treatment, many rod photoreceptors retract their terminals, while cone pedicles form clusters

retina rod cell degeneration ( J:186624 )

• with horizontal cell ablation, many rod photoreceptors are lost, while cone number is not significantly affected

disorganized retina outer plexiform layer ( J:186624 )

• at 8 weeks and 6 months after DT treatment, normal columnar alignment of photoreceptor nuclei is disrupted; about one third of photoreceptor nuclei are lost in treated mice

thin retina outer plexiform layer ( J:186624 )

• after diphtheria toxin (DT) injection to retinal ablate horizontal cells in adult animals, the outer plexiform layer appears thinner at 2 weeks after treatment and is almost completely absent at 6 months

abnormal eye electrophysiology ( J:186624 )

• under scotopic conditions, 6 months after treatment, a-wave amplitudes are significantly lower than in wild-type retina for intensities >0.5 cds/sq. meter and b-wave amplitudes are lower than wild-type for intensities >0.005 cds/sq. meter

• photopic electroretinograms show similar effects with the b-wave increasing with increasing light intensities in wild-type but not mutants

• ON responses (time to peak) are slower in retinae of treated mice while OFF responses are not significantly different, indicating that ganglion cell responses in treated mice are just mildly affected

abnormal vision ( J:186624 )

• visual performance (visual acuity and contrast sensitivity) in DT-treated mutants is slightly lower than in wild-type despite morphology changes in outer retina