Phenotypes associated with this allele

• Allele Symbol: Tgm3^tm1Sjo
• Allele Name: targeted mutation 1, Susan John
• Allele ID: MGI:5436399
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tgm3^tm1Sjo/Tgm3^tm1Sjo	involves: 129P2/OlaHsd * C57BL/6	MGI:5436400

Genotype
MGI:5436400

hm1

• Allelic Composition: Tgm3^tm1Sjo/Tgm3^tm1Sjo
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Wavy hair and irregular vibrissae in Tgm3^tm1Sjo/Tgm3^tm1Sjo mice

integument

abnormal hair texture ( J:187109 )

• adults have an increase in the number of uneven, thinner hairs with irregular torsions

brittle hair ( J:187109 )

waved hair ( J:187109 )

• most obvious in the first four weeks of life

abnormal hair shaft morphology ( J:187109 )

• the even groove present in wild-type hairs is often absent or highly deformed

• hair integrity appears to be compromised as overnight treatment with detergent and a reducing agent results in large areas of cuticle loss and, in places, medulla distortion

• the Huxely's layer is often torn or distorted in pelage hair and whiskers

abnormal hair cortex keratinization ( J:187109 )

• keratin filaments are often shorter and less regular compared to wild-type controls

abnormal hair cuticle ( J:187109 )

• overlapping pattern of the scales is often distorted

• small cracks in the cuticle or scales lifting away from the cortex are sometimes seen

increased curvature of hairs ( J:187109 )

• irregular twisting of many hairs

abnormal zigzag hair morphology ( J:187109 )

• appear brittle

decreased zigzag hair amount ( J:187109 )

• at 4 months of age

abnormal vibrissa morphology ( J:187109 )

• detectable by P2

• thinner than in controls

• the Huxely's layer is often torn or distorted in pelage hair and whiskers

curly vibrissae ( J:187109 )

• twisted

loss of vibrissae ( J:187109 )

delayed skin barrier formation ( J:187109 )

• retain toluidine blue particularly in the abdominal area at E17.5 suggesting a delay in barrier formation as no defects are seen in neonates or adults

abnormal skin physiology ( J:187109 )

• corneocytes isolated from the pinna are more susceptible to sonication in reducing conditions suggesting a decrease in stability

impaired skin barrier function ( J:203964 )

• impairment of the skin barrier funtion is demonstrated by FITC penetration from the skin surface, followed by two-photon microscopy showing a more invasive percutaneous penetration in homozygous mice

• increased cutaneous inflammation is shown after skin FITC treatment and increased ear swelling is shown after FITC sensitization

• no difference is seen in mouse ear swelling test (MEST) between homozygous mutant and control mice after intradermal Propionibacter acnes injection, suggesting that FITC-induced inflammation is a consequence of an impaired skin barrier than an increased activity of the mutant immune system

homeostasis/metabolism

impaired skin barrier function ( J:203964 )

• impairment of the skin barrier funtion is demonstrated by FITC penetration from the skin surface, followed by two-photon microscopy showing a more invasive percutaneous penetration in homozygous mice

• increased cutaneous inflammation is shown after skin FITC treatment and increased ear swelling is shown after FITC sensitization

• no difference is seen in mouse ear swelling test (MEST) between homozygous mutant and control mice after intradermal Propionibacter acnes injection, suggesting that FITC-induced inflammation is a consequence of an impaired skin barrier than an increased activity of the mutant immune system