Phenotypes associated with this allele

• Allele Symbol: Ccl17^{tm2.1(cre)Ifo}
• Allele Name: targeted mutation 2.1, Irmgard Foerster
• Allele ID: MGI:5438830
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ccl17^tm2.1(cre)Ifo/Ccl17^+ Hif1a^tm3Rsjo/Hif1a^tm3Rsjo	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5545808

Genotype
MGI:5545808

cn1

• Allelic Composition: Ccl17^{tm2.1(cre)Ifo}/Ccl17⁺; Hif1a^tm3Rsjo/Hif1a^tm3Rsjo
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:187773 )

N • bone marrow derived dendritic cells (BMDCs) cultured under hypoxic conditions (1% oxygen) show upregulation of maturation markers such as MHCII, CD86, with CD80 only slightly enhanced; control BMDCs grown in hypoxic conditions show similar enhanced maturation markers

N • cytokine production is reduced in mutant and control BMDCs under hypoxic conditions; production of Il12p70, Il10, Il6, and Il23 is decreased in mutant and control BMDCs under hypoxic conditions, with Il22 upregulated compared to normoxic cells

N • stimulation by LPS does not further enhance expression of maturation markers as it does in BMDCs grown under normoxic conditions

N • production of Il12p70, Il10, Il6, and Il23 is decreased in mutant and control BMDCs under hypoxic conditions, with Il22 upregulated compared to normoxic cells

abnormal leukocyte migration ( J:187773 )

• fewer mutant BMDCs generated under hypoxic conditions migrate toward CCL19 in a transwell chamber assay than control cells grown under hypoxic conditions or mutant and control cells generated under normoxic conditions; migration toward CXCL12 is not different from controls under hypoxic or normoxic conditions

abnormal dendritic cell number ( J:187773 )

• bone marrow dendritic cells (BMDCs) differentiated under hypoxic conditions in culture display reduced growth (proliferation) compared to control cells grown in hypoxia or mutant cells grown under normoxic (21% oxygen) conditions

abnormal interleukin-1 beta secretion ( J:187773 )

• BMDCs produce similar levels of Il-1b under hypoxic and normoxic conditions while control cells and BMDCs with CD11c-driven Hif1a deletion show decreased production under hypoxic conditions

abnormal tumor necrosis factor secretion ( J:187773 )

• BMDCs produce similar levels of TNFalpha under hypoxic and normoxic conditions while control cells and BMDCs with CD11c-driven Hif1a deletion show decreased production under hypoxic conditions

cellular

abnormal cell physiology ( J:187773 )

• reduced amounts of ATP are detected in lysates of mutant BMDCs cultured under hypoxic conditions compared to control cells under hypoxia suggesting an energy metabolism defect with Hif1a deletion

abnormal leukocyte migration ( J:187773 )

• fewer mutant BMDCs generated under hypoxic conditions migrate toward CCL19 in a transwell chamber assay than control cells grown under hypoxic conditions or mutant and control cells generated under normoxic conditions; migration toward CXCL12 is not different from controls under hypoxic or normoxic conditions

hematopoietic system

abnormal leukocyte migration ( J:187773 )

• fewer mutant BMDCs generated under hypoxic conditions migrate toward CCL19 in a transwell chamber assay than control cells grown under hypoxic conditions or mutant and control cells generated under normoxic conditions; migration toward CXCL12 is not different from controls under hypoxic or normoxic conditions

abnormal dendritic cell number ( J:187773 )

• bone marrow dendritic cells (BMDCs) differentiated under hypoxic conditions in culture display reduced growth (proliferation) compared to control cells grown in hypoxia or mutant cells grown under normoxic (21% oxygen) conditions