Phenotypes associated with this allele

• Allele Symbol: Htr1a^tm3.1Rhn
• Allele Name: targeted mutation 3.1, Rene Hen
• Allele ID: MGI:5439287
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Htr1a^tm3.1Rhn/? Tg(HCRT-tTA)G5Ahky/0	B6.Cg-Htr1a^tm3.1Rhn Tg(HCRT-tTA)G5Ahky	MGI:5582938
cx2	Htr1a^tm3.1Rhn/Htr1a^tm3.1Rhn Slc6a4^tm1(tTA)Cogr/Slc6a4^+	involves: 129S1/Sv * BALB/cJ * C57BL/6 * CBA	MGI:3802744

Genotype
MGI:5582938

cx1

• Allelic Composition: Htr1a^tm3.1Rhn/?; Tg(HCRT-tTA)G5Ahky/0
• Genetic Background: B6.Cg-Htr1a^tm3.1Rhn Tg(HCRT-tTA)G5Ahky

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal sleep duration ( J:212974 )

• the mean wakefulness bout duration is shortened

abnormal sleep pattern ( J:212974 )

• however, the sleep/wakefulness pattern during the light period is not affected

• the transition frequency between wakefulness and sleep is increased in the first 3 hours of the dark period

• in the presence of DOX, mice show normal sleep/wakefulness patterns

increased slow-wave sleep duration ( J:212974 )

• total amounts of slow wave sleep are increased during the first 6 hours of the dark period

• however, mean slow wave sleep bout durations are unaffected

increased frequency of paradoxical sleep ( J:212974 )

• total amounts of REM sleep are increased during the first 6 hours of the dark period

fragmentation of sleep/wake states ( J:212974 )

• mice show severe fragmentation of wakefulness during the first half of the dark period, with a decrease in total time in wakefulness

Genotype
MGI:3802744

cx2

• Allelic Composition: Htr1a^tm3.1Rhn/Htr1a^tm3.1Rhn; Slc6a4^tm1(tTA)Cogr/Slc6a4⁺
• Genetic Background: involves: 129S1/Sv * BALB/cJ * C57BL/6 * CBA

mortality/aging

decreased survivor rate ( J:137439 )

• 30% of mice survive beyond P120

• fewer mice die when over-expression begins at P60 instead of P40

premature death ( J:137439 )

• most mice die before 3 months of age frequently between P25 and P80

• in 37% of mice, crises of severe bradycardia and hypothermia progress to death

• however, 30% of mice survive beyond P120 and treatment with doxycycline prevents premature death

nervous system

abnormal autonomic nervous system physiology ( J:137439 )

• mice exhibit sporadic autonomic crisis, involving bradycardia preceded 5 to 10 minutes by hypothermia, that persists for several hours and is followed by several days of recovery or, in the case of 37% of mice, progresses to death

abnormal neuron physiology ( J:137439 )

• following treatment of brain slices with tryptophan, cells exhibit reduced firing compared to wild-type cells

• however, normal cell firing can be re-established by treatment with doxycycline

cardiovascular system

decreased heart rate ( J:137439 )

• mice exhibit sporadic autonomic crises involving a decrease in heart rate and temperature than persists for several hours followed by several days of recovery

• bradycardia precedes hypothermia by 5 to 10 minutes

homeostasis/metabolism

decreased body temperature ( J:137439 )

• mice exhibit sporadic autonomic crises involving a decrease in heart rate and temperature than persists for several hours followed by several days of recovery

• bradycardia precedes hypothermia by 5 to 10 minutes