Analysis Tools
reproductive system
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• both genes are expressed in ovaria, but ovulation, fertilization, preimplantation embryo development and hormone production are normal in conditional loss-of-function females
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• persistent apicobasal polarity in the luminal epithelium with increased cell heights and retention of glycosylated microvilli on the apical surface
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• abnormal primary decidual zone (PDZ) development at 6 dpc
• PDZ was absent in some implantation sites with the embryo entrapped within the intact epithelium at 6 dpc
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• uteri either failed to show or had very weak endometrial vascular permeability at the site of blastocyst attachment at 5 dpc
• unattached blastocysts were recovered from uteri at 5 dpc
• defects in crypt formation
• some blastocysts situated within the primary lumen
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• inappropriate mesometrial-antimesometrial (M-AM) orientation of implantation sites at 5, 6 and 8 dpc: closer to M pole instead of at AM pole
• ratio of M-AM axis length to anterior-posterior (A-P) axis length is reduced by ~25% resulting in a more spherical morphology at 8 dpc
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• uteri either failed to show or had very weak endometrial vascular permeability at the site of blastocyst attachment at 5 dpc
• different sizes of implantation sites at 8 dpc
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• implantation sites were often irregularly spaced at 5 and 8 dpc
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• two embryos closely apposed within one implantation site
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• only 33% of plug-positive females produce a (small) litter
• high resorption rates
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• in the few fertile mice
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embryo
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• clusters of Prl3d1-positive trophoblast giant cells in implantation sites in lieu of the embryo proper at 10 dpc
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• increasing numbers of resorption sites on 10 and 12 dpc
• absent or substantially smaller embryos in many implantation sites from 10 dpc
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• in surviving implantation sites at 12 dpc
• disrupted placental development from 10 dpc
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• abnormal primary decidual zone (PDZ) development at 6 dpc
• PDZ was absent in some implantation sites with the embryo entrapped within the intact epithelium at 6 dpc
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• Gcm1-positive syncytiotrophoblasts in the labyrinth failed to elongate, suggesting disrupted secondary branching morphogenesis of placental villi at 12 dpc
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cellular
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• clusters of Prl3d1-positive trophoblast giant cells in implantation sites in lieu of the embryo proper at 10 dpc
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