Phenotypes associated with this allele

• Allele Symbol: Eef2^tm1.1Lepp
• Allele Name: targeted mutation 1.1, Stephen H Leppla
• Allele ID: MGI:5441712
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Eef2^tm1.1Lepp/Eef2^tm1.1Lepp	involves: C57BL/6	MGI:5441713
ht2	Eef2^tm1.1Lepp/Eef2^+	involves: C57BL/6	MGI:5441714
cx3	Dph1^tm1Bhr/Dph1^tm1Bhr Eef2^tm1.1Lepp/Eef2^tm1.1Lepp	involves: 129S4/SvJae * C57BL/6	MGI:5441716

Genotype
MGI:5441713

hm1

• Allelic Composition: Eef2^tm1.1Lepp/Eef2^tm1.1Lepp
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Delayed development in Eef2^tm1.1Lepp/Eef2^tm1.1Lepp mice

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:188600 )

• resistant to lethality induced by 5 ug PA + 5 ug FP59

• all survive up to 8 doses

neonatal lethality, incomplete penetrance ( J:188600 )

• most mice die shortly after birth

• at weaning only 1.2% of mice are homozygous rather than the expected 25%

• most mice that survive birth appear healthy, are fertile and survive to adulthood

embryo

abnormal embryonic tissue physiology ( J:188600 )

• absence of ADP-ribosylation in tissues

cellular

cellular phenotype ( J:188600 )

N • protein synthesis rates in MEFS are similar to those in control MEFs

abnormal cell physiology ( J:188600 )

• partially resistant to the decrease in protein synthesis rates induced by anthrax protective antigen + fusion protein 59 (PA + FP59)

• increase in the protein synthesis error rate due to -1 frameshifts in MEFs

decreased sensitivity to induced cell death ( J:188600 )

• MEFs are highly resistant to PA + FP59 induced cell death during 48 h toxin incubations

• only modest cell death (about 30%) is seen after 200 h incubations

growth/size/body

decreased fetal size ( J:188600 )

• at E17.5

fetal growth retardation ( J:188600 )

• developmental delay is detected after E14.5

Genotype
MGI:5441714

ht2

• Allelic Composition: Eef2^tm1.1Lepp/Eef2⁺
• Genetic Background: involves: C57BL/6

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:188600 )

• resistant to lethality induced by 5 ug PA + 5 ug FP59

• all survive 1 dose and 60% survive up to 8 doses

cellular

decreased sensitivity to induced cell death ( J:188600 )

• MEFs are highly resistant to PA + FP59 induced cell death during 48 h toxin incubations

• however, all cells die after 120 h incubations

Genotype
MGI:5441716

cx3

• Allelic Composition: Dph1^tm1Bhr/Dph1^tm1Bhr; Eef2^tm1.1Lepp/Eef2^tm1.1Lepp
• Genetic Background: involves: 129S4/SvJae * C57BL/6

mortality/aging

mortality/aging ( J:188600 )

N • many surviving adults are detected unlike in mice homozygous for Dph1^tm1Bhr alone