All Phenotypes Zbtb46<tm1(HBEGF)Mnz> MGI Mouse

N

• diphtheria-treated bone marrow chimeras exhibit normal numbers of NK cells and splenic red pulp macrophages

• diphtheria toxin-treated chimeras infected with L. monocytogenes exhibit normal numbers of activated monocytes compared with control mice

• diphtheria-treated mice exhibit normal numbers of F4/80+ red pulp macrophages, CD169+ marginal zone macrophages and lymph node subscapular sinus macrophages

increased neutrophil cell number ( J:189122 )

• diphtheria toxin-treated bone marrow chimeras exhibit increased numbers of splenic Ly6G+ neutrophils compared with controls

decreased dendritic cell number ( J:189122 )

• absence of classical dendritic cells in diphtheria toxin-treated bone marrow chimeras

• partial loss of CD11c^hiMHCII+ dendritic cells of the small intestine lamina propria of diphtheria toxin-treated bone marrow chimeras

• reduced Lin-CD11c^hi classical dendritic cells in diphtheria-treated T. gondii-infected bone marrow chimeras

• however, plasmacytoid cell numbers are normal in diphtheria toxin-treated bone marrow chimeras

increased monocyte cell number ( J:189122 )

• small increase in diphtheria toxin-treated bone marrow chimeras

abnormal T-helper 1 physiology ( J:189122 )

• reduced IFNgamma+ CD3+CD4+ T cells in diphtheria toxin-treated bone marrow chimeras immunized with either alpha-DEC-205-GAGp24 or alpha-Treml4-GAGp24, or infected with T. gondii

abnormal memory T cell physiology ( J:189122 )

• diphtheria toxin-treated bone marrow chimeras exhibit reduced antitumor memory response compared with control mice that is not as severe as in Tg(Itgax-DTR/EGFP)57Lan hemizygotes

abnormal response to infection ( J:189122 )

• diphtheria toxin-treated chimeras infected with L. monocytogenes exhibit fewer activated monocytes compared with control mice

• mice treated with diphtheria toxin and LPS fail to accumulate CD11c+MHCII+CD14+DEC-205- and CD11c+MHCII+DC-SIGN/CD209+CD14+ cells in the skin lymph nodes unlike control mice

• however, diphtheria toxin-treated chimeras infected with L. monocytogenes exhibit normal numbers of activated monocytes compared with control mice

increased susceptibility to parasitic infection ( J:189122 )

• diphtheria toxin-treated mice infected with T. gondii exhibit decreased IFNgamma+ CD3+CD4+ T cells and increased parasitic burden compared with controls that is not as severe as in Tg(Itgax-DTR/EGFP)57Lan hemizygotes