Phenotypes associated with this allele

• Allele Symbol: Cd300a^tm1Shib
• Allele Name: targeted mutation 1, Akira Shibuya
• Allele ID: MGI:5445723
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd300a^tm1Shib/Cd300a^tm1Shib	B6JJcl.C-Cd300a^tm1Shib	MGI:5445741

Genotype
MGI:5445741

hm1

• Allelic Composition: Cd300a^tm1Shib/Cd300a^tm1Shib
• Genetic Background: B6JJcl.C-Cd300a^tm1Shib

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased susceptibility to induced morbidity/mortality ( J:189144 )

• following cecal ligation and puncture, mice exhibit longer survival compared with wild-type mice

• however, antibody-mediated depletion of neutrophils normalizes survival

immune system

increased neutrophil cell number ( J:189144 )

• from peritoneal resident macrophages cocultured with apoptotic cells in the presence of LPS

abnormal mast cell physiology ( J:189144 )

• bone marrow-derived mast cells stimulated with LPS secrete less TNFalpha, IL13 and MCP-1 compared with wild-type cells

• however, secretion from bone marrow-derived mast cells is normal in the presence of the D89E variant of MFG-E8

abnormal chemokine secretion ( J:189144 )

• bone marrow-derived mast cells stimulated with LPS secrete less MCP-1 (CCL2) compared with wild-type cells

• however, secretion from bone marrow-derived mast cells is normal in the presence of the D89E variant of MFG-E8

increased interleukin-10 secretion ( J:189144 )

• from peritoneal resident macrophages cocultured with apoptotic cells in the presence of LPS

increased interleukin-13 secretion ( J:189144 )

• from bone marrow-derived mast cells stimulated with LPS

• however, secretion from bone marrow-derived mast cells is normal in the presence of the D89E variant of MFG-E8

increased tumor necrosis factor secretion ( J:189144 )

• from bone marrow-derived mast cells stimulated with LPS

• from peritoneal resident macrophages cocultured with apoptotic cells in the presence of LPS

• however, secretion from bone marrow-derived mast cells is normal in the presence of the D89E variant of MFG-E8

homeostasis/metabolism

decreased susceptibility to injury ( J:189144 )

• following cecal ligation and puncture, mice exhibit longer survival, reduced bacterial colony-forming unit and increased neutrophil numbers in the peritoneal cavity compared with wild-type mice

• decreased susceptibility to cecal ligation and puncture can be conferred to Kit^W-sh by bone marrow-derived mast cells

• however, antibody-mediated depletion of neutrophils normalizes survival

hematopoietic system

increased neutrophil cell number ( J:189144 )

• from peritoneal resident macrophages cocultured with apoptotic cells in the presence of LPS

abnormal mast cell physiology ( J:189144 )

• bone marrow-derived mast cells stimulated with LPS secrete less TNFalpha, IL13 and MCP-1 compared with wild-type cells

• however, secretion from bone marrow-derived mast cells is normal in the presence of the D89E variant of MFG-E8