Phenotypes associated with this allele

• Allele Symbol: Ngf^tm1.1Blhe
• Allele Name: targeted mutation 1.1, Barbara L Hempstead
• Allele ID: MGI:5461488
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Ngf^tm1.1Blhe/Ngf^+	B6.Cg-Ngf^tm1.1Blhe	MGI:5461510
cx2	Ngf^tm1.1Blhe/Ngf^+ Ngfr^tm1Jae/Ngfr^tm1Jae	B6.Cg-Ngf^tm1.1Blhe Ngfr^tm1Jae	MGI:5461512
cx3	Ngf^tm1.1Blhe/Ngf^+ Sort1^tm1Tew/Sort1^tm1Tew	B6.Cg-Ngf^tm1.1Blhe Sort1^tm1Tew	MGI:5461513
cx4	Ngf^tm1.1Blhe/Ngf^+ Tg(Cspg4-DsRed.T1)1Akik/0	B6.Cg-Ngf^tm1.1Blhe Tg(Cspg4-DsRed.T1)1Akik	MGI:5461511

Genotype
MGI:5461510

ht1

• Allelic Composition: Ngf^tm1.1Blhe/Ngf⁺
• Genetic Background: B6.Cg-Ngf^tm1.1Blhe

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Adult Ngf^tm1.1Blhe/Ngf⁺ mice exhibit dilated cardiomyopathy and fibrosis and this phentoype is rescued in Ngfr^tm1Jae/Ngfr^tm1Jae Ngf^tm1.1Blhe/Ngf⁺ mice

mortality/aging

premature death ( J:190893 )

• some mice die by 5 months

• most mice die before 8 months

cardiovascular system

abnormal vascular endothelial cell morphology ( J:190893 )

• many endothelial cells exhibit attenuated cytoplasm with occasional overt rupture and extravasation of erythrocytes unlike in wild-type mice

abnormal myocardium layer morphology ( J:190893 )

• myocardial fibrosis at 8 month without myocyte cell death

abnormal myocardial fiber morphology ( J:190893 )

• focal myofibrillar damage with focal collagen depositions

thin myocardium ( J:190893 )

• at 8 month

abnormal heart left ventricle morphology ( J:190893 )

• decreased left ventricular posterior wall thickness at diastole and systole

dilated heart left ventricle ( J:190893 )

• at 8 month

dilated heart right ventricle ( J:190893 )

• at 8 month

dilated cardiomyopathy ( J:190893 )

decreased cardiac muscle contractility ( J:190893 )

• contractile dysfunction at 2 months

abnormal vascular endothelial cell physiology ( J:190893 )

• mice exhibit microvascular endothelial activation and increased vascular permeability prior to the development of cardiac fibrosis

increased vascular permeability ( J:190893 )

homeostasis/metabolism

edema ( J:190893 )

• perivascular edema in capillary beds

muscle

abnormal myocardium layer morphology ( J:190893 )

• myocardial fibrosis at 8 month without myocyte cell death

abnormal myocardial fiber morphology ( J:190893 )

• focal myofibrillar damage with focal collagen depositions

thin myocardium ( J:190893 )

• at 8 month

dilated cardiomyopathy ( J:190893 )

decreased cardiac muscle contractility ( J:190893 )

• contractile dysfunction at 2 months

Genotype
MGI:5461512

cx2

• Allelic Composition: Ngf^tm1.1Blhe/Ngf⁺; Ngfr^tm1Jae/Ngfr^tm1Jae
• Genetic Background: B6.Cg-Ngf^tm1.1Blhe Ngfr^tm1Jae

Adult Ngf^tm1.1Blhe/Ngf⁺ mice exhibit dilated cardiomyopathy and fibrosis and this phentoype is rescued in Ngfr^tm1Jae/Ngfr^tm1Jae Ngf^tm1.1Blhe/Ngf⁺ mice

cardiovascular system

cardiovascular system phenotype ( J:190893 )

N • unlike Ngf^tm1.1Blhe heterozygotes, mice do not exhibit ventricular dilation, fibrosis cardiac hypocontractility or premature death

Genotype
MGI:5461513

cx3

• Allelic Composition: Ngf^tm1.1Blhe/Ngf⁺; Sort1^tm1Tew/Sort1^tm1Tew
• Genetic Background: B6.Cg-Ngf^tm1.1Blhe Sort1^tm1Tew

mortality/aging

premature death ( J:190893 )

• most mice die by 4 months

• nearly all mice die by 8 months

cardiovascular system

dilated cardiomyopathy ( J:190893 )

muscle

dilated cardiomyopathy ( J:190893 )

Genotype
MGI:5461511

cx4

• Allelic Composition: Ngf^tm1.1Blhe/Ngf⁺; Tg(Cspg4-DsRed.T1)1Akik/0
• Genetic Background: B6.Cg-Ngf^tm1.1Blhe Tg(Cspg4-DsRed.T1)1Akik

cardiovascular system

abnormal pericyte morphology ( J:190893 )

• shorter pericyte processes with reduced pericyte coverage of the cardiac microvasculature