Phenotypes associated with this allele

• Allele Symbol: Tg(Thy1-APPArc)M8Cagr
• Allele Name: transgene insertion M8, Caroline Graff
• Allele ID: MGI:5463435
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(Thy1-APPArc)M8Cagr/Tg(Thy1-APPArc)M8Cagr	involves: C57BL/6 * CBA	MGI:5465558
tg2	Tg(Thy1-APPArc)M8Cagr/0	involves: C57BL/6 * CBA	MGI:5463438

Genotype
MGI:5465558

tg1

• Allelic Composition: Tg(Thy1-APPArc)M8Cagr/Tg(Thy1-APPArc)M8Cagr
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

amyloid beta deposits ( J:188191 )

• both males and females exhibit amyloid beta deposits in the brain, however females show more severe neuropathological alternations than males when compared at the same age and thus all further analysis was performed with females

• females exhibit intracellular amyloid beta aggregates in subiculum and pyramidal cells in CA1 and cortical layer V at 3 months of age, accumulation of diffuse extracellular amyloid beta in subiculum, alveus, and the retropsplenial granular cortex at 4 months of age, diffuse amyloid beta accumulation in thalamus, mammillary bodies, fimbria, fornix, motor cortex, and sensory cortex at 9 months of age, and additional diffuse accumulation in the entorhinal cortex, piriform cortex, lateral septum, and pons/medulla at 12-15 months of age

• females exhibit amyloid beta 40 and amyloid beta 42 immunoreactivity in plaque-like structures in subiculum at 6 months of age, and additionally in the thalamus, alveus, mammillary bodies, lateral septum, and pons/medulla, and around vessel walls in thalamus and subiculum at 12-15 months of age

homeostasis/metabolism

amyloid beta deposits ( J:188191 )

• both males and females exhibit amyloid beta deposits in the brain, however females show more severe neuropathological alternations than males when compared at the same age and thus all further analysis was performed with females

• females exhibit intracellular amyloid beta aggregates in subiculum and pyramidal cells in CA1 and cortical layer V at 3 months of age, accumulation of diffuse extracellular amyloid beta in subiculum, alveus, and the retropsplenial granular cortex at 4 months of age, diffuse amyloid beta accumulation in thalamus, mammillary bodies, fimbria, fornix, motor cortex, and sensory cortex at 9 months of age, and additional diffuse accumulation in the entorhinal cortex, piriform cortex, lateral septum, and pons/medulla at 12-15 months of age

• females exhibit amyloid beta 40 and amyloid beta 42 immunoreactivity in plaque-like structures in subiculum at 6 months of age, and additionally in the thalamus, alveus, mammillary bodies, lateral septum, and pons/medulla, and around vessel walls in thalamus and subiculum at 12-15 months of age

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Alzheimer's disease		DOID:10652		J:188191

Genotype
MGI:5463438

tg2

• Allelic Composition: Tg(Thy1-APPArc)M8Cagr/0
• Genetic Background: involves: C57BL/6 * CBA

behavior/neurological

behavior/neurological phenotype ( J:168288 )

N • mutants exhibit normal motor activity, exploratory behavior, and vertical activity in the open-field test at all ages tested

abnormal spatial learning ( J:168288 )

♀ • in the Barnes maze, mutant females exhibit longer latency to find the target hole during the acquisition phase and the reversal learning phase compared to wild-type females, however no differences are seen between mutant and wild-type males, indicating impaired spatial learning and memory in 15 month old females

abnormal long-term spatial reference memory ( J:168288 )

♀ • in the Barnes maze, mutant females exhibit longer latency to find the target hole during the reversal learning phase compared to wild-type females, indicating impaired long-term learning and memory

abnormal spatial working memory ( J:168288 )

• in a delayed alternation test, mutants show a longer latency to find the escape platform at 15 months of age compared to wild-type mice, indicating a minor impairment in working memory

nervous system

amyloid beta deposits ( J:168288 )

• amyloid beta plaques are evident in the subiculum and thalamus at 7 months and 18 months of age, respectively

• amyloid beta deposition is seen around vessel walls and in the parenchyma

• females exhibit higher levels of amyloid beta deposits than males

homeostasis/metabolism

amyloid beta deposits ( J:168288 )

• amyloid beta plaques are evident in the subiculum and thalamus at 7 months and 18 months of age, respectively

• amyloid beta deposition is seen around vessel walls and in the parenchyma

• females exhibit higher levels of amyloid beta deposits than males

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Alzheimer's disease		DOID:10652		J:168288