reproductive system
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• at P6, TRA98+ spermatogonial stem cells (SSCs) are completely depleted in tubules
• at P0, numbers of TRA98+ germ cells (gonocytes) per unit area are reduced by 42%
• male germ cells are gradually lost from E18.5 to P6
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• no sperm are released from the cauda epididymides in adult males
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• single-cell transcriptome analysis showed that genes involved in cell cycle and glial cell line- derived neurotrophic factor (GDNF) pathway are significantly reduced in gonocytes
• at P2, testis sections show a significantly lower % of Gfra1+ gonocytes per unit area than control sections (2% versus 79%), indicating impaired gonocytes-to-spermatogonia transition
• at P2, testis sections show a significantly lower % of Ki67+ gonocytes per unit area than control sections (0.7% versus 41%), indicating impaired proliferation of gonocytes
• however, no increase in gonocyte apoptosis is detected by TUNEL staining at P0 or P2
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• all seminiferous tubules are atrophic and empty in adult testes
• at P90, tubules are completely devoid of TRA98+ germ cells and contain only SOX9+ Sertoli cells
• at P6, TRA98+ SSCs are completely depleted in tubules, resulting in a Sertoli cell-only phenotype
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• adult testes are significantly smaller than control testes
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• adult testis to body weight ratio is significantly lower than that in control males
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• adult males have no capacity to produce germ cells; no stage-specific subpopulations of spermatogenic cells are detected
• disruption of spermatogenesis occurs prior to spermatogonia formation
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• no mature sperm are found in the cauda epididymides of adult males
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• 3-mo-old male mice fail to produce offspring when mated with wild-type females
• however, copulatory plugs are observed
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cellular
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• at P6, TRA98+ spermatogonial stem cells (SSCs) are completely depleted in tubules
• at P0, numbers of TRA98+ germ cells (gonocytes) per unit area are reduced by 42%
• male germ cells are gradually lost from E18.5 to P6
|
|
|
• no sperm are released from the cauda epididymides in adult males
|
|
|
• single-cell transcriptome analysis showed that genes involved in cell cycle and glial cell line- derived neurotrophic factor (GDNF) pathway are significantly reduced in gonocytes
• at P2, testis sections show a significantly lower % of Gfra1+ gonocytes per unit area than control sections (2% versus 79%), indicating impaired gonocytes-to-spermatogonia transition
• at P2, testis sections show a significantly lower % of Ki67+ gonocytes per unit area than control sections (0.7% versus 41%), indicating impaired proliferation of gonocytes
• however, no increase in gonocyte apoptosis is detected by TUNEL staining at P0 or P2
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endocrine/exocrine glands
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• all seminiferous tubules are atrophic and empty in adult testes
• at P90, tubules are completely devoid of TRA98+ germ cells and contain only SOX9+ Sertoli cells
• at P6, TRA98+ SSCs are completely depleted in tubules, resulting in a Sertoli cell-only phenotype
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• adult testes are significantly smaller than control testes
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• adult testis to body weight ratio is significantly lower than that in control males
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Analysis Tools