Phenotypes associated with this allele

• Allele Symbol: Dot1l^tm1.1Saam
• Allele Name: targeted mutation 1.1, Scott Armstrong
• Allele ID: MGI:5468988
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dot1l^tm1.1Saam/Dot1l^tm1.1Saam	involves: 129S1/Sv * C57BL/6	MGI:5468991
cn2	Dot1l^tm1.1Saam/Dot1l^tm1.1Saam	involves: 129S1/Sv * C57BL/6	MGI:5468992
cn3	Dot1l^tm1.1Saam/Dot1l^tm1.1Saam Tg(VAV1-cre)1Graf/0	involves: 129S1/Sv * C57BL/6	MGI:5468993

Genotype
MGI:5468991

hm1

• Allelic Composition: Dot1l^tm1.1Saam/Dot1l^tm1.1Saam
• Genetic Background: involves: 129S1/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:193161 )

• mice are phenotypically normal with normal blood counts

Genotype
MGI:5468992

cn2

• Allelic Composition: Dot1l^tm1.1Saam/Dot1l^tm1.1Saam
• Genetic Background: involves: 129S1/Sv * C57BL/6

hematopoietic system

abnormal bone marrow cell morphology/development ( J:193161 )

• bone marrow cells transformed with a MLL-AF9 retrovirus then a cre-expressing retrovirus produce decreased numbers of smaller blast-like colonies on methylcellulose compared with control cells

• however, myeloid progenitor assays are normal in cells not transformed with MLL-AF9 or transformed with HoxA9/Meis1a

abnormal bone marrow cell physiology ( J:193161 )

• bone marrow cells transformed with MLL-AF9-expressing retrovirus, transduced with a cre-expressing retrovirus and transplanted into irradiated recipients fail to cause leukemia unlike control cells

neoplasm

decreased tumor growth/size ( J:193161 )

• transplanted MLL-AF9 leukemia cells transduced with a cre-expressing vector form fewer, smaller and less compact colony composed of terminally differentiated cells with reduced cell proliferation compared with control cells

Genotype
MGI:5468993

cn3

• Allelic Composition: Dot1l^tm1.1Saam/Dot1l^tm1.1Saam; Tg(VAV1-cre)1Graf/0
• Genetic Background: involves: 129S1/Sv * C57BL/6

hematopoietic system

abnormal definitive hematopoiesis ( J:193161 )

• moderate to severe reduction in hematopoietic stem cells, granulocyte-macrophage progenitors and common myeloid progenitors with less of an affect on megakaryocyte/erythroid progenitors

decreased common myeloid progenitor cell number ( J:193161 )

• moderately to severely

abnormal leukopoiesis ( J:193161 )

• moderately to severely reduction in granulocyte-macrophage progenitors

anemia ( J:193161 )

• varying degrees in young mice

impaired hematopoiesis ( J:193161 )

• but not absent

decreased bone marrow cell number ( J:193161 )

decreased neutrophil cell number ( J:193161 )

• mild to moderate in young mice

decreased lymphocyte cell number ( J:193161 )

• mild to moderate in young mice

decreased hematopoietic stem cell number ( J:193161 )

• moderately to severely

immune system

abnormal leukopoiesis ( J:193161 )

• moderately to severely reduction in granulocyte-macrophage progenitors

decreased neutrophil cell number ( J:193161 )

• mild to moderate in young mice

decreased lymphocyte cell number ( J:193161 )

• mild to moderate in young mice