Phenotypes associated with this allele

• Allele Symbol: Rr96^tm1.1Tich
• Allele Name: targeted mutation 1.1, Tian Chi
• Allele ID: MGI:5470225
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rr96^tm1.1Tich/Rr96^+ Gt(ROSA)26Sor^tm1(cre/ERT2)Thl/Gt(ROSA)26Sor^tm1(rtTA*M2)Jae	involves: 129 * 129S4/SvJae * C57BL/6	MGI:5470231
cx2	Rr96^tm1.1Tich/Rr96^+ Gt(ROSA)26Sor^tm1(rtTA*M2)Jae/Gt(ROSA)26Sor^+	involves: 129 * 129S4/SvJae * C57BL/6	MGI:5470229
cx3	Rr96^tm1.1Tich/Rr96^tm1.1Tich Gt(ROSA)26Sor^tm1(rtTA*M2)Jae/Gt(ROSA)26Sor^tm1(rtTA*M2)Jae	involves: 129 * 129S4/SvJae * C57BL/6	MGI:5470230
cx4	Rr96^tm1.1Tich/Rr96^tm1.1Tich Tg(ACTB-tTS)1Mllo/?	involves: 129 * C57BL/6	MGI:5470228

Genotype
MGI:5470231

cn1

• Allelic Composition: Rr96^tm1.1Tich/Rr96⁺; Gt(ROSA)26Sor^{tm1(cre/ERT2)Thl}/Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}
• Genetic Background: involves: 129 * 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal T cell differentiation ( J:191711 )

• derepression of Cd4 occurs in all CD4^- T cells

• T cell expression of CD4 increases rapidly after cre activation

hematopoietic system

abnormal T cell differentiation ( J:191711 )

• derepression of Cd4 occurs in all CD4^- T cells

• T cell expression of CD4 increases rapidly after cre activation

Genotype
MGI:5470229

cx2

• Allelic Composition: Rr96^tm1.1Tich/Rr96⁺; Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129 * 129S4/SvJae * C57BL/6

immune system

abnormal CD4-positive T cell differentiation ( J:191711 )

• 15 days of doxycycline exposure leads to CD4 induction in all but the earliest CD4^- thymocytes (not in DN1-3)

• only about 30% of peripheral CD8 cells begin expressing CD4

hematopoietic system

abnormal CD4-positive T cell differentiation ( J:191711 )

• 15 days of doxycycline exposure leads to CD4 induction in all but the earliest CD4^- thymocytes (not in DN1-3)

• only about 30% of peripheral CD8 cells begin expressing CD4

Genotype
MGI:5470230

cx3

• Allelic Composition: Rr96^tm1.1Tich/Rr96^tm1.1Tich; Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}/Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae}
• Genetic Background: involves: 129 * 129S4/SvJae * C57BL/6

immune system

abnormal T cell differentiation ( J:191711 )

• doxycycline treatment leads to detectable CD4⁺ CD8⁺ cells after 2 days

• levels of double positive cells reach 47% by 17 days after the start of treatment

hematopoietic system

abnormal T cell differentiation ( J:191711 )

• doxycycline treatment leads to detectable CD4⁺ CD8⁺ cells after 2 days

• levels of double positive cells reach 47% by 17 days after the start of treatment

Genotype
MGI:5470228

cx4

• Allelic Composition: Rr96^tm1.1Tich/Rr96^tm1.1Tich; Tg(ACTB-tTS)1Mllo/?
• Genetic Background: involves: 129 * C57BL/6

immune system

decreased double-positive T cell number ( J:191711 )

• double positive T cells are converted to CD4^- CD8⁺ TCR^-/lo cells

• a population of TCR⁺ CD4^- CD8^-/lo cells is found in both the thymus and the periphery

• doxycycine treatmen reverses T cell changes in the thymus but not in peripheral T cells

hematopoietic system

decreased double-positive T cell number ( J:191711 )

• double positive T cells are converted to CD4^- CD8⁺ TCR^-/lo cells

• a population of TCR⁺ CD4^- CD8^-/lo cells is found in both the thymus and the periphery

• doxycycine treatmen reverses T cell changes in the thymus but not in peripheral T cells