Phenotypes associated with this allele

• Allele Symbol: Slc17a8^tm1(cre)Lowl
• Allele Name: targeted mutation 1, Bradford B Lowell
• Allele ID: MGI:5471228
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Runx1^tm3Spe/Runx1^tm3Spe Slc17a8^tm1(cre)Lowl/Slc17a8^+	involves: 129S4/SvJae	MGI:5471253
cn2	Slc17a8^tm1(cre)Lowl/Slc17a8^+ Runx1^tm3Spe/Runx1^tm3Spe	involves: 129S4/SvJae * 129S6/SvEvTac * C57BL/6NCr	MGI:5471252

Genotype
MGI:5471253

cn1

• Allelic Composition: Runx1^tm3Spe/Runx1^tm3Spe; Slc17a8^tm1(cre)Lowl/Slc17a8⁺
• Genetic Background: involves: 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:193903 )

N • thermal nociception, chemical nociception, and neuropathic mechanical pain are not markedly affected in mutants

N • mechanical allodynia is unaffected in mutants

hypoalgesia ( J:193903 )

• intraplantar injection of carrageenan still induces mechanical hypersensitivity in mutants, but a modest (significant) increase in mechanical thresholds compared to controls is observed (higher magnitude of mechanical stimulus required to evoke paw withdrawal)

Genotype
MGI:5471252

cn2

• Allelic Composition: Slc17a8^tm1(cre)Lowl/Slc17a8⁺; Runx1^tm3Spe/Runx1^tm3Spe
• Genetic Background: involves: 129S4/SvJae * 129S6/SvEvTac * C57BL/6NCr

nervous system

abnormal sensory neuron innervation pattern ( J:193903 )

• most sensory neurons innervating hair follicles of the hairy skin display tdTomato +ve unmyelinated circumferential endings in contrast to the longitudinal unmyelinated lanceolate endings observed in control skin; these circumferential endings are located ventral to tdTomato -ve, NF200 +ve myelinated (non-sensory) circumferential endings

• around hairs in the epidermis no decrease in tdTomato +ve free nerve endings is observed; morphology of dermal papillae-epidermis nerve endings in thick glabrous skin is unchanged

• innervation of the touch dome by tdTomato labeled myelinated mechanoreceptors is not changed

decreased sensory neuron number ( J:193903 )

• mutants show a marked loss of mechanosensitive neurons compared to controls, with a concurrent increase in the number of nonsensitive neurons

abnormal sympathetic neuron physiology ( J:193903 )

• average current density in remaining mechanosensitive small neurons is reduced compared to control mice, but no change in the average current density is detected in medium/large tdTomato-labeled neurons