Phenotypes associated with this allele

• Allele Symbol: Cfl1^c5
• Allele Name: c5
• Allele ID: MGI:5473701
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cfl1^c5/Cfl1^c5	involves: C57BL/6J	MGI:5473702
cx2	Cfl1^c5/Cfl1^c5 Dstn^corn1/Dstn^corn1	involves: A/WySn * C57BL/6J	MGI:5473707
cx3	Cfl1^c5/Cfl1^c5 Dstn^corn1/Dstn^+	involves: A/WySn * C57BL/6J	MGI:5473708
cx4	Cfl1^c5/Cfl1^c5 Vangl2^Lp/Vangl2^Lp	involves: C57BL/6J * LPT/LeJ	MGI:5473703

Genotype
MGI:5473702

hm1

• Allelic Composition: Cfl1^c5/Cfl1^c5
• Genetic Background: involves: C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

open neural tube ( J:194042 )

• open cranial neural tube but closed in the trunk at E9.5

wavy neural tube ( J:194042 )

cardiovascular system

abnormal cardiac outflow tract development ( J:194042 )

pericardial edema ( J:194042 )

nervous system

open neural tube ( J:194042 )

• open cranial neural tube but closed in the trunk at E9.5

wavy neural tube ( J:194042 )

exencephaly ( J:194042 )

• open cranial neural tube but closed in the trunk at E9.5

homeostasis/metabolism

pericardial edema ( J:194042 )

Genotype
MGI:5473707

cx2

• Allelic Composition: Cfl1^c5/Cfl1^c5; Dstn^corn1/Dstn^corn1
• Genetic Background: involves: A/WySn * C57BL/6J

mortality/aging

embryonic lethality before implantation, complete penetrance ( J:194042 )

• unable to recover embryos at post-implantation stages

Genotype
MGI:5473708

cx3

• Allelic Composition: Cfl1^c5/Cfl1^c5; Dstn^corn1/Dstn⁺
• Genetic Background: involves: A/WySn * C57BL/6J

mortality/aging

embryonic lethality ( J:194042 )

• most arrest prior to node formation

embryo

embryonic growth arrest ( J:194042 )

• most arrest prior to node formation

abnormal primitive node morphology ( J:194042 )

• in the 3 embryos that develop nodes apical trafficking of planar cell polarity proteins is disrupted

Genotype
MGI:5473703

cx4

• Allelic Composition: Cfl1^c5/Cfl1^c5; Vangl2^Lp/Vangl2^Lp
• Genetic Background: involves: C57BL/6J * LPT/LeJ

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:194042 )

• arrest at approximately E9.5

embryo

abnormal embryo turning ( J:194042 )

• fail to undergo turning

abnormal left-right axis patterning ( J:194042 )

• at E8.0 - E8.5 expression analysis indicates randomized left-right axis patterning

embryonic growth arrest ( J:194042 )

• at approximately E9.5

decreased embryo size ( J:194042 )

• shorter at E9.5

open neural tube ( J:194042 )

• open along the entire axis of the embryo at E9.5

abnormal notochord morphology ( J:194042 )

• midline is shorter and wider at the 1 to 4 somite stage compared to either single mutant

• threefold shorter and wider compared to wild-type controls, twofold shorter and wider compared to Vangl2^Lp single mutants

• change in size is not due to changes in cell shape

abnormal primitive node morphology ( J:194042 )

• expression analysis indicates many node cells lack posterior polarization

abnormal embryonic cilium location or orientation ( J:194042 )

• cilia are normal in length but do not point uniformly to the posterior and their position on the cell is more variable than in controls

abnormal somite development ( J:194042 )

abnormal somite size ( J:194042 )

• significantly reduced length to width ratio compared to either single mutant

cardiovascular system

abnormal heart looping ( J:194042 )

abnormal direction of heart looping ( J:194042 )

• in 2 of 6 embryos at E9.5

failure of heart looping ( J:194042 )

• a linear heart tube is seen at E9.5 in 1 of 6 embryos

growth/size/body

decreased embryo size ( J:194042 )

• shorter at E9.5

heterotaxia ( J:194042 )

• at E8.0 - E8.5 expression analysis indicates randomized left-right axis patterning

nervous system

open neural tube ( J:194042 )

• open along the entire axis of the embryo at E9.5

cellular

abnormal embryonic cilium location or orientation ( J:194042 )

• cilia are normal in length but do not point uniformly to the posterior and their position on the cell is more variable than in controls