Phenotypes associated with this allele

• Allele Symbol: Tg(CD68-icre)1Bwlx
• Allele Name: transgene insertion 1, Ben Wielockx
• Allele ID: MGI:5474196
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Egln1^tm1.1Brei/Egln1^tm1.1Brei Hif1a^tm3Rsjo/Hif1a^tm3Rsjo Tg(CD68-icre)1Bwlx/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5568401
cn2	Hif1a^tm3Rsjo/Hif1a^tm3Rsjo Tg(CD68-icre)1Bwlx/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:5568404
cn3	Egln1^tm1.1Brei/Egln1^tm1.1Brei Egln3^tm2Fong/Egln3^tm2Fong Tg(CD68-icre)1Bwlx/0	involves: 129S6/SvEvTac * C57BL/6	MGI:5568402
cn4	Egln1^tm1.1Brei/Egln1^tm1.1Brei Epas1^tm1Mcs/Epas1^tm1Mcs Tg(CD68-icre)1Bwlx/0	involves: 129X1/SvJ * C57BL/6	MGI:5568400
cn5	Epas1^tm1Mcs/Epas1^tm1Mcs Tg(CD68-icre)1Bwlx/0	involves: 129X1/SvJ * C57BL/6	MGI:5568403
cn6	Egln1^tm1.1Brei/Egln1^tm1.1Brei Tg(CD68-icre)1Bwlx/0	involves: C57BL/6	MGI:5568399

Genotype
MGI:5568401

cn1

• Allelic Composition: Egln1^tm1.1Brei/Egln1^tm1.1Brei; Hif1a^tm3Rsjo/Hif1a^tm3Rsjo; Tg(CD68-icre)1Bwlx/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality ( J:194598 )

• mice start dying suddenly at 5 weeks with 95% mortality by 16 weeks (mean survival time = 11 weeks)

hematopoietic system

hematopoietic system phenotype ( J:194598 )

N • no cardiovascular complications or thromboses are observed

increased hematocrit ( J:194598 )

• not different from CD68cre/Phd2^fl mice

craniofacial

domed cranium ( J:194598 )

• 3/16 mice show this

nervous system

hydrocephaly ( J:194598 )

• mice with domed skulls show dramatic hydrocephalus

skeleton

domed cranium ( J:194598 )

• 3/16 mice show this

Genotype
MGI:5568404

cn2

• Allelic Composition: Hif1a^tm3Rsjo/Hif1a^tm3Rsjo; Tg(CD68-icre)1Bwlx/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

mortality/aging

mortality/aging ( J:194598 )

N • no premature death observed

Genotype
MGI:5568402

cn3

• Allelic Composition: Egln1^tm1.1Brei/Egln1^tm1.1Brei; Egln3^tm2Fong/Egln3^tm2Fong; Tg(CD68-icre)1Bwlx/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

hematopoietic system

polycythemia ( J:194598 )

• mice display severe erythrocytosis

mortality/aging

neonatal lethality ( J:194598 )

• animals start dying shortly after birth

Genotype
MGI:5568400

cn4

• Allelic Composition: Egln1^tm1.1Brei/Egln1^tm1.1Brei; Epas1^tm1Mcs/Epas1^tm1Mcs; Tg(CD68-icre)1Bwlx/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6

mortality/aging

mortality/aging ( J:194598 )

N • no premature death is observed

hematopoietic system

hematopoietic system phenotype ( J:194598 )

N • erythropoiesis phenotype seen in Phd2 single mutants is absent from double mutants

decreased hematocrit ( J:194598 )

• HCT is lower than wild type

decreased hemoglobin content ( J:194598 )

• lower than in wild-type

Genotype
MGI:5568403

cn5

• Allelic Composition: Epas1^tm1Mcs/Epas1^tm1Mcs; Tg(CD68-icre)1Bwlx/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6

mortality/aging

mortality/aging ( J:194598 )

N • no premature death observed

Genotype
MGI:5568399

cn6

• Allelic Composition: Egln1^tm1.1Brei/Egln1^tm1.1Brei; Tg(CD68-icre)1Bwlx/0
• Genetic Background: involves: C57BL/6

mortality/aging

mortality/aging ( J:194598 )

N • no premature death is observed; some erythrocytotic mutants live for more than 20 months with no observed decrease in hematocrit

growth/size/body

postnatal growth retardation ( J:194598 )

• severe growth retardation occurs in conjunction with skin redness until adulthood

enlarged spleen ( J:194598 )

increased spleen weight ( J:194598 )

hematopoietic system

hematopoietic system phenotype ( J:194598 )

N • F4/80+ macrophages and neutrophils are not significantly different from wild type, but red pulp macrophages are highly induced in the spleen

abnormal megakaryocyte progenitor cell morphology ( J:194598 )

• megakaryocyte-erythroid progenitors (MEPs) are significantly induced (>2.5 fold) in the spleen, but not in bone marrow

increased erythroblast number ( J:194598 )

• splenic CD71+ve/Ter119+ve erythroblasts are increase >10-fold with mild increase in bone marrow

polycythemia ( J:194598 )

• results in splenomegaly

increased hematocrit ( J:194598 )

• increases with age to >85% in some animals

abnormal nucleated erythrocyte cell number ( J:194598 )

• splenomegaly occurs with increase in nucleated cells

thrombocytopenia ( J:194598 )

• significantly decreased

abnormal lymphocyte cell number ( J:194598 )

• fraction of B and T cells in spleen is significantly decreased but in lymph nodes these numbers are increased

abnormal spleen morphology ( J:194598 )

• splenomegaly occurs with loss of splenic architecture

enlarged spleen ( J:194598 )

increased spleen weight ( J:194598 )

cardiovascular system

abnormal blood viscosity ( J:194598 )

• viscosity is increased but no differences in heart rate, blood pressure or cardiac hypertrophy is observed

hypervolemia ( J:194598 )

• blood volume increased by 65%

immune system

abnormal lymphocyte cell number ( J:194598 )

• fraction of B and T cells in spleen is significantly decreased but in lymph nodes these numbers are increased

abnormal spleen morphology ( J:194598 )

• splenomegaly occurs with loss of splenic architecture

enlarged spleen ( J:194598 )

increased spleen weight ( J:194598 )

integument

reddish skin ( J:194598 )

• redness of paws and snouts is observed by 4 weeks of age